DOI QR코드

DOI QR Code

A standardized bamboo leaf extract inhibits monocyte adhesion to endothelial cells by modulating vascular cell adhesion protein-1

  • Choi, Sunga (Infectious Signaling Network Research Center and Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Park, Myoung Soo (Infectious Signaling Network Research Center and Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Lee, Yu Ran (Infectious Signaling Network Research Center and Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University) ;
  • Lee, Young Chul (Unigen Inc.) ;
  • Kim, Tae Woo (Unigen Inc.) ;
  • Do, Seon-Gil (Life Science Research Institute, Univera Inc.) ;
  • Kim, Dong Seon (Unigen Inc.) ;
  • Jeon, Byeong Hwa (Infectious Signaling Network Research Center and Research Institute for Medical Sciences, Department of Physiology, School of Medicine, Chungnam National University)
  • Received : 2012.09.04
  • Accepted : 2012.12.07
  • Published : 2013.02.01

Abstract

Bamboo leaves (Phyllostachys pubescens Mazel ex J. Houz (Poacea)) have a long history of food and medical applications in Asia, including Japan and Korea. They have been used as a traditional medicine for centuries. We investigated the mechanism of anti-inflammatory activity of a bamboo leaf extract (BLE) on tumor necrosis factor-alpha (TNF-${\alpha}$)-induced monocyte adhesion in human umbilical vein endothelial cells (HUVECs). Exposure of HUVECs to BLE did not inhibit cell viability or cause morphological changes at concentrations ranging from 1 ${\mu}g/ml$ to 1 mg/ml. Treatment with 0.1 mg/ml BLE caused 63% inhibition of monocyte adhesion in TNF-${\alpha}$-activated HUVECs, which was associated with 38.4% suppression of vascular cell adhesion molecule-1 expression. Furthermore, TNF-${\alpha}$-induced reactive oxygen species generation was decreased to 47.9% in BLE treated TNF-${\alpha}$-activated HUVECs. BLE (0.05 mg/ml) also caused about 50% inhibition of interleukin-6 secretion from lipopolysaccharide-stimulated monocyte. The results indicate that BLE may be clinically useful as an anti-inflammatory or anti-oxidant for human cardiovascular disease including atherosclerosis.

Keywords

Bamboo leaf extracts;anti-inflammation;reactive oxygen species;interleukin-6;endothelial cells

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

References

  1. Roy S, Sen CK, Packer L. Determination of cell-cell adhesion in response to oxidants and antioxidants. Methods Enzymol 1999; 300:395-401. https://doi.org/10.1016/S0076-6879(99)00144-5
  2. Bevilacqua MP, Pober JS, Wheeler ME, Cotran RS, Gimbrone MA Jr. Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines. J Clin Invest 1985; 76:2003-11. https://doi.org/10.1172/JCI112200
  3. Faruqi RM, DiCorleto PE. Mechanisms of monocyte recruitment and accumulation. Br Heart J 1993;69:S19-29. https://doi.org/10.1136/hrt.69.1_Suppl.S19
  4. Lum H, Roebuck KA. Oxidant stress and endothelial cell dysfunction. Am J Physiol Cell Physiol 2001;280:C719-41. https://doi.org/10.1152/ajpcell.2001.280.4.C719
  5. Chen YH, Lin SJ, Ku HH, Shiao MS, Lin FY, Chen JW, Chen YL. Salvianolic acid B attenuates VCAM-1 and ICAM-1 expression in TNF-alpha-treated human aortic endothelial cells. J Cell Biochem 2001;82:512-21. https://doi.org/10.1002/jcb.1176
  6. Hung CF, Huang TF, Chen BH, Shieh JM, Wu PH, Wu WB. Lycopene inhibits TNF-alpha-induced endothelial ICAM-1 expression and monocyte-endothelial adhesion. Eur J Pharmacol 2008;586: 275-82. https://doi.org/10.1016/j.ejphar.2008.03.001
  7. Murphy N, Grimsditch DC, Vidgeon-Hart M, Groot PH, Overend P, Benson GM, Graham A. Dietary antioxidants decrease serum soluble adhesion molecule (sVCAM-1, sICAM-1) but not chemokine (JE/MCP-1, KC) concentrations, and reduce atherosclerosis in C57BL but not apoE*3 Leiden mice fed an atherogenic diet. Dis Markers 2005;21:181-90. https://doi.org/10.1155/2005/394152
  8. Lakota K, Mrak-Poljsak K, Rozman B, Sodin-Semrl S. Increased responsiveness of human coronary artery endothelial cells in inflammation and coagulation. Mediators Inflamm 2009;2009: 146872.
  9. Islam KN, Devaraj S, Jialal I. alpha-Tocopherol enrichment of monocytes decreases agonist-induced adhesion to human endothelial cells. Circulation 1998;98:2255-61. https://doi.org/10.1161/01.CIR.98.21.2255
  10. Steeber DA, Tedder TF. Adhesion molecule cascades direct lymphocyte recirculation and leukocyte migration during inflammation. Immunol Res 2000;22:299-317. https://doi.org/10.1385/IR:22:2-3:299
  11. Wackers FJ, Soufer R, Zaret BL. Nuclear cardiology. In: Braunwald E, Zipes D, Libby P, editors. Heart Disease: A Textbook of Cardiovascular Medicine. 6th ed. Philadelphia: Saunders; 2001. p.288-90.
  12. Campbell JH, Efendy JL, Smith NJ, Campbell GR. Molecular basis by which garlic suppresses atherosclerosis. J Nutr 2001; 131:1006S-1009S. https://doi.org/10.1093/jn/131.3.1006S
  13. Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet 1993;342:1007-11. https://doi.org/10.1016/0140-6736(93)92876-U
  14. Knekt P, Jarvinen R, Reunanen A, Maatela J. Flavonoid intake and coronary mortality in Finland: a cohort study. BMJ 1996; 312:478-81. https://doi.org/10.1136/bmj.312.7029.478
  15. Grassi D, Aggio A, Onori L, Croce G, Tiberti S, Ferri C, Ferri L, Desideri G. Tea, flavonoids, and nitric oxide-mediated vascular reactivity. J Nutr 2008;138:1554S-1560S. https://doi.org/10.1093/jn/138.8.1554S
  16. Shah AJ, Gilani AH. Aqueous-methanolic extract of sweet flag (Acorus calamus) possesses cardiac depressant and endothelialderived hyperpolarizing factor-mediated coronary vasodilator effects. J Nat Med 2012;66:119-26. https://doi.org/10.1007/s11418-011-0561-7
  17. Kweon MH, Hwang HJ, Sung HC. Identification and antioxidant activity of novel chlorogenic acid derivatives from bamboo (Phyllostachys edulis). J Agric Food Chem 2001;49:4646-55. https://doi.org/10.1021/jf010514x
  18. Park HS, Lim JH, Kim HJ, Choi HJ, Lee IS. Antioxidant flavone glycosides from the leaves of Sasa borealis. Arch Pharm Res 2007;30:161-6. https://doi.org/10.1007/BF02977689
  19. Seki T, Maeda H. Cancer preventive effect of Kumaizasa bamboo leaf extracts administered prior to carcinogenesis or cancer inoculation. Anticancer Res 2010;30:111-8.
  20. Kim CS, Son SJ, Kim EK, Kim SN, Yoo DG, Kim HS, Ryoo SW, Lee SD, Irani K, Jeon BH. Apurinic/apyrimidinic endonuclease1/ redox factor-1 inhibits monocyte adhesion in endothelial cells. Cardiovasc Res 2006;69:520-6. https://doi.org/10.1016/j.cardiores.2005.10.014
  21. Cho EJ, Park MS, Kim SS, Kang G, Choi S, Lee YR, Chang SJ, Lee KH, Lee SD, Park JB, Jeon BH. Vasorelaxing activity of Ulmus davidiana ethanol extracts in rats: activation of endothelial nitric oxide synthase. Korean J Physiol Pharmacol 2011;15:339-44. https://doi.org/10.4196/kjpp.2011.15.6.339
  22. Kim SN, Son SC, Lee SM, Kim CS, Yoo DG, Lee SK, Hur GM, Park JB, Jeon BH. Midazolam inhibits proinflammatory mediators in the lipopolysaccharide-activated macrophage. Anesthesiology 2006;105:105-10. https://doi.org/10.1097/00000542-200607000-00019
  23. Weber KS, von Hundelshausen P, Clark-Lewis I, Weber PC, Weber C. Differential immobilization and hierarchical involvement of chemokines in monocyte arrest and transmigration on inflamed endothelium in shear flow. Eur J Immunol 1999;29:700-12. https://doi.org/10.1002/(SICI)1521-4141(199902)29:02<700::AID-IMMU700>3.0.CO;2-1
  24. Gabay C. Interleukin-6 and chronic inflammation. Arthritis Res Ther 2006;8 Suppl 2:S3.
  25. Cao LH, Lee YJ, Kang DG, Kim JS, Lee HS. Effect of Zanthoxylum schinifolium on TNF-alpha-induced vascular inflammation in human umbilical vein endothelial cells. Vascul Pharmacol 2009;50:200-7. https://doi.org/10.1016/j.vph.2009.01.008
  26. Flavahan NA. Atherosclerosis or lipoprotein-induced endothelial dysfunction. Potential mechanisms underlying reduction in EDRF/nitric oxide activity. Circulation 1992;85:1927-38. https://doi.org/10.1161/01.CIR.85.5.1927
  27. Harrison DG. Endothelial dysfunction in atherosclerosis. Basic Res Cardiol 1994;89 Suppl 1:87-102.
  28. Calabresi PA, Prat A, Biernacki K, Rollins J, Antel JP. T lymphocytes conditioned with Interferon beta induce membrane and soluble VCAM on human brain endothelial cells. J Neuroimmunol 2001;115:161-7. https://doi.org/10.1016/S0165-5728(01)00253-3
  29. James WG, Bullard DC, Hickey MJ. Critical role of the alpha 4 integrin/VCAM-1 pathway in cerebral leukocyte trafficking in lupus-prone MRL/fas(lpr) mice. J Immunol 2003;170:520-7. https://doi.org/10.4049/jimmunol.170.1.520
  30. Ley K, Huo Y. VCAM-1 is critical in atherosclerosis. J Clin Invest 2001;107:1209-10. https://doi.org/10.1172/JCI13005
  31. Eriksson EE, Xie X, Werr J, Thoren P, Lindbom L. Importance of primary capture and L-selectin-dependent secondary capture in leukocyte accumulation in inflammation and atherosclerosis in vivo. J Exp Med 2001;194:205-18. https://doi.org/10.1084/jem.194.2.205
  32. McEver RP. Adhesive interactions of leukocytes, platelets, and the vessel wall during hemostasis and inflammation. Thromb Haemost 2001;86:746-56.

Cited by

  1. Anthocyanin-Rich Purple Corn Extract Inhibit Diabetes-Associated Glomerular Angiogenesis vol.8, pp.11, 2013, https://doi.org/10.1371/journal.pone.0079823
  2. 竹叶提取物改善老年痴呆模型大鼠的空间学习能力 vol.16, pp.7, 2015, https://doi.org/10.1631/jzus.B1400249
  3. Bamboo (Phyllostachys bambusoides) leaf extracts inhibit adipogenesis by regulating adipogenic transcription factors and enzymes in 3T3-L1 adipocytes vol.26, pp.4, 2017, https://doi.org/10.1007/s10068-017-0150-y
  4. vol.9, pp.4, 2018, https://doi.org/10.1039/C7FO01666B