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Allergenic potential and enzymatic resistance of buckwheat

  • Lee, Sujin ;
  • Han, Youngshin ;
  • Do, Jeong-Ryong ;
  • Oh, Sangsuk
  • Received : 2012.09.25
  • Accepted : 2012.12.11
  • Published : 2013.02.01

Abstract

Buckwheat is known as a health food but is one of the major food allergens triggering potentially fatal anaphylaxis in Asia, especially in Japan and Korea. This study was conducted to investigate the characteristic of enzymatic resistance of buckwheat protein and allergenic potential. Enzymatic resistance of buckwheat protein was performed with in vitro digestibility test in simulated gastric fluid (SGF), pH 1.2, using pepsin and simulated intestinal fluid (SIF) using chymotrypsin. Reactivity of buckwheat proteins to human IgE was performed using six allergic patients sensitized to buckwheat. Buckwheat's IgE levels were measured using the Phadia UniCAP-system. Buckwheat protein, 16 kDa, still remained after 30 min treatment of pepsin on SDS-PAGE. Even though 16 kDa almost disappeared after 60 min treatment, two out of the six buckwheat patients' sera showed reactivity to hydrolysate after 60 min treatment, indicating that allergenicity still remained. In simulated intestinal fluid (SIF) using chymotrypsin, buckwheat protein, 24 kDa, showed resistance to hydrolysis with chymotrypsin on SDS-PAGE, and still had allergenicity based on the result of ELISA. Our results suggest that buckwheat proteins have strong resistance to enzyme degradation. This may be attributed in part to the allergenic potential of buckwheat. Further study should be continued regarding buckwheat allergy.

Keywords

Buckwheat;hydrolysis;pepsin;chymotrypsin;allergenicity

References

  1. IUIS Allergen Nomenclature [Internet]. Allergens of Buckwheat. [cited 2012 July 14]. Available from: http://www.allergen.org/.
  2. Takagi K, Teshima R, Okunuki H, Sawada J. Comparative study of in vitro digestibility of food proteins and effect of preheating on the digestion. Biol Pharm Bull 2003;26:969-73. https://doi.org/10.1248/bpb.26.969
  3. Koyano S, Takagi K, Teshima R, Sawada J. Molecular cloning of cDNA, recombinant protein expression and characterization of a buckwheat 16-kDa major allergen. Int Arch Allergy Immunol 2006;140:73-81. https://doi.org/10.1159/000092038
  4. Satoh R, Koyano S, Takagi K, Nakamura R, Teshima R, Sawada J. Immunological characterization and mutational analysis of the recombinant protein BWp16, a major allergen in buckwheat. Biol Pharm Bull 2008;31:1079-85. https://doi.org/10.1248/bpb.31.1079
  5. The Korean Academy of Asthma. Allergy and Clinical Immunology, 4 Chunmanyoi Allergy. Seoul: Yoihakmunhwasa Publishing Co.; 2005. p.85-92.
  6. Smith HL. Buckwheat-poisoning with report of a case in man. Arch Intern Med (Chic) 1909;3:350-9.
  7. Son DY, Yoon KR, Lee SI. Study of the most common allergic foods in Korea. Korean J Food Sci Technol 2002;34:885-8.
  8. Houben GF, Knippels LM, Penninks AH. Food allergy: predictive testing of food products. Environ Toxicol Pharmacol 1997;4:127-35. https://doi.org/10.1016/S1382-6689(97)10053-9
  9. Tanaka K, Matsumoto K, Akasawa A, Nakajima T, Nagasu T, Iikura Y, Saito H. Pepsin-resistant 16-kD buckwheat protein is associated with immediate hypersensitivity reaction in patients with buckwheat allergy. Int Arch Allergy Immunol 2002;129: 49-56. https://doi.org/10.1159/000065173
  10. Kruger NJ. The bradford method for protein quantitation. In: Walker JM, editor. The Protein Protocols Handbook. 2nd ed. Totowa: Humana Press Inc.; 2002. p.15-21.
  11. Kato H, van Chuyen N, Utsunomiya N, Okitani A. Changes of amino acids composition and relative digestibility of lysozyme in the reaction with alpha-dicarbonyl compounds in aqueous system. J Nutr Sci Vitaminol (Tokyo) 1986;32:55-65. https://doi.org/10.3177/jnsv.32.55
  12. Keum EH, Lee SI, Oh S. Effect of enzymatic hydrolysis of 7S globulin, a soybean protein, on its allergenicity and identification of its allergenic hydrolyzed fragments using SDS-PAGE. Food Sci Biotechnol 2006;15:128-32.
  13. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-5. https://doi.org/10.1038/227680a0
  14. Matsumoto R, Fujino K, Nagata Y, Hashiguchi S, Ito Y, Aihara Y, Takahashi Y, Maeda K, Sugimura K. Molecular characterization of a 10-kDa buckwheat molecule reactive to allergic patients' IgE. Allergy 2004;59:533-8. https://doi.org/10.1046/j.1398-9995.2003.00412.x
  15. Park JW, Kang DB, Kim CW, Ko SH, Yum HY, Kim KE, Hong CS, Lee KY. Identification and characterization of the major allergens of buckwheat. Allergy 2000;55:1035-41. https://doi.org/10.1034/j.1398-9995.2000.00763.x
  16. Nair A, Adachi T. Immunodetection and characterization of allergenic proteins in common buckwheat (Fagopyrum esculentum). Plant Biotechnol (Tsukuba) 1999;16:219-24. https://doi.org/10.5511/plantbiotechnology.16.219
  17. Kondo Y, Urisu A, Wada E, Tsuruta M, Yasaki T, Yamada K, Masuda S, Morita Y. Allergen analysis of buckwheat by the immunoblotting method. Arerugi 1993;42:142-8.
  18. Urisu A, Kondo Y, Morita Y. Identification of a major allergen of buckwheat seeds by immunoblotting methods. Allergy Clin Immunol News 1994;6:151-5.
  19. Han Y, Kim J, Ahn K. Food allergy. Korean J Pediatr 2012;55: 153-8. https://doi.org/10.3345/kjp.2012.55.5.153
  20. Yoshimasu MA, Zhang JW, Hayakawa S, Mine Y. Electrophoretic and immunochemical characterization of allergenic proteins in buckwheat. Int Arch Allergy Immunol 2000;123:130-6. https://doi.org/10.1159/000024431
  21. Satoh R, Koyano S, Takagi K, Nakamura R, Teshima R. Identification of an IgE-binding epitope of a major buckwheat allergen, BWp16, by SPOTs assay and mimotope screening. Int Arch Allergy Immunol 2010;153:133-40. https://doi.org/10.1159/000312630
  22. Wieslander G, Norback D. Buckwheat allergy. Allergy 2001;56: 703-4. https://doi.org/10.1034/j.1398-9995.2001.056008703.x
  23. Park CH, Choi YS. Memil. Chuncheon: Kangwon National University Publishing Co.; 2004. p.35-43.
  24. He J, Klag MJ, Whelton PK, Mo JP, Chen JY, Qian MC, Mo PS, He GQ. Oats and buckwheat intakes and cardiovascular disease risk factors in an ethnic minority of China. Am J Clin Nutr 1995;61:366-72. https://doi.org/10.1093/ajcn/61.2.366
  25. Choe M, Kim JD, Park KS, Oh SY, Lee SY. Effect of buckwheat supplementation on blood glucose levels and blood pressure in rats. J Korean Soc Food Nutr 1991;20:300-5.

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  2. Significance of 40-, 45-, and 48-kDa Proteins in the Moderate-to-Severe Clinical Symptoms of Buckwheat Allergy vol.7, pp.1, 2015, https://doi.org/10.4168/aair.2015.7.1.37
  3. 54. Buckwheat – the world’s most biodiversity-friendly crop? vol.18, pp.2-3, 2017, https://doi.org/10.1080/14888386.2017.1332529
  4. Dynamic changes in polyphenol compounds, antioxidant activity, and PAL gene expression in different tissues of buckwheat during germination vol.98, pp.15, 2018, https://doi.org/10.1002/jsfa.9119
  5. Buckwheat anaphylaxis: a case report vol.10, pp.S2, 2014, https://doi.org/10.1186/1710-1492-10-S2-A38

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)