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Clinical, Cytogenetic and CYP1A1 exon-1 Gene Mutation Analysis of Beedi Workers in Vellore Region, Tamil Nadu

  • Sundaramoorthy, Rajiv (Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University) ;
  • Srinivasan, Vasanth (Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University) ;
  • Gujar, Jidnyasa (Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University) ;
  • Sen, Ayantika (Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University) ;
  • Sekar, Nishu (Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University) ;
  • Abilash, Valsala Gopalakrishnan (Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University)
  • Published : 2013.12.31

Abstract

Background: Beedi rollers are exposed to unburnt tobacco dust through cutaneous and pharyngeal route and it is extremely harmful to the body since it is carcinogenic in nature and can cause cancer during long exposure. This indicates that occupational exposure to tobacco imposes considerable genotoxicity among beedi workers. Materials and Methods: In the present study, 27 beedi workers and age and sex matched controls were enrolled for clinical, cytogenetics and molecular analysis. Clinical features were recorded. The workers were in the age group of 28-67 years and were workers exposure from 8-60 years. Blood samples were collected from workers and control subjects and lymphocyte cultures were carried out by using standard technique, slides were prepared and 50 metaphases were scored for each sample to find the chromosomal abnormalities. For molecular analysis the genomic DNA was extracted from peripheral blood, to screen the variations in gene, the exon 1 of CYP1A1 gene was amplified by polymerase chain reaction (PCR) and then screened with Single Strand Conformation Polymorphism (SSCP) analysis. Results: A statistically significant increase was observed in the frequencies of chromosomal aberrations in exposed groups when compared to the respective controls and variations observed in Exon 1 of CYP1A1(Cytochrome P450, family 1, subfamily A, polypeptide 1) gene. Conclusions: This study shows that, the toxicants present in the beedi that enter into human body causes disturbance to normal state and behavior of the chromosomes which results in reshuffling of hereditary material causing chromosomal aberrations and genomic variations.

References

  1. Bagwe AN, Bhisey RA (1995). Occupational exposure to unburnt bidi tobacco elevates mutagenic burden among tobacco processors. Carcinogenesis, 16, 1095-9. https://doi.org/10.1093/carcin/16.5.1095
  2. Bagwe AN, Ramchandani AG, Bhisey RA (1994). Skin-tumourpromoting activity of processed bidi tobacco in hairless S/RV Cri-ba mice. J Cancer Res Clin Oncol, 120, 485-9. https://doi.org/10.1007/BF01191802
  3. Blair A, Berney BW, Heid MF (1983). Causes of death among workers in the tobacco industry. Arch Environ Health, 38, 223-8. https://doi.org/10.1080/00039896.1983.10545807
  4. Bozina N, Bradamante V, Lovric M (2009). Genetic polymorphism of metabolic enzymes P450 (CYP) as a susceptibility factor for drug response, toxicity, and cancer risk. Arh Hig Rada Toksikol, 60, 217-42.
  5. Berber U, Yilmaz I, Yilmaz O, (2013). CYP1A1 (Ile462Val), CYP1B1 (Ala119Ser and Val432Leu), GSTM1 (null), and GSTT1 (null) polymorphisms and bladder cancer risk in a Turkish population. Asian Pac J Cancer Prev, 14, 3925-9. https://doi.org/10.7314/APJCP.2013.14.6.3925
  6. Chattopadhyay BP, Kundu SA (2006). Study to assess the respiratory impairments among the male beedi workers in unorganized sectors. Indian J Occup Environ Med, 10, 69-73. https://doi.org/10.4103/0019-5278.27303
  7. Chen YY, Bye J, Yuang W (2006). Association of polymorphisms in AhR, CYP1A1, GSTM1 and GSTT1 genes with levels of DNA damage in peripheral blood lymphocytes among coke-oven workers. Cancer Epidemiol Biomarkers Prev, 15, 1703-7. https://doi.org/10.1158/1055-9965.EPI-06-0291
  8. Cribb AE, Knight MJ, Dryer D (2006). Role of polymorphic human cytochrome p450 enzymes in estrone oxidation. Cancer Epidemiol Biomar, 15, 551-8. https://doi.org/10.1158/1055-9965.EPI-05-0801
  9. Cribb AE, Knight JM, Guernsey J (2011). Cyp17, catechol-omethyltransferase, and glutathione transferase m1 genetic polymorphisms, lifestyle factors, and breast cancer risk in women on Prince Edward Island. Breast J, 17, 24-31. https://doi.org/10.1111/j.1524-4741.2010.01025.x
  10. Dong LM, Potter JD, White E, et al (2008). Genetic susceptibility to cancer: the role of polymorphisms in candidate genes. JAMA, 299, 2423-36. https://doi.org/10.1001/jama.299.20.2423
  11. Ghosh PC, Iqbal R, Bairwa BL, Roy C, Sudarshan C (2005). Occupational Health profile of the beedi workers and ergonomic intervention. Indosh News, 10, 8-13.
  12. Giri SK, Yadav A, Kumar A, et al (2012). CYP1A1 Gene polymorphisms: modulator of genetic damage in coal-tar workers. Asian Pac J Cancer Prev, 13, 3409-16. https://doi.org/10.7314/APJCP.2012.13.7.3409
  13. Hayashi SI, Watanabe J, Nakachi K, Kawajiri K (1991). PCR detection of an A/G polymorphism within exon 7 of the CYP1A1 gene. Nucleic Acids Res, 19, 47-97.
  14. Hungerford DA (1965). Leucocytes cultured from small inocula of whole blood and the preparation of metaphase chromosomes by treatment with hypotonic KCl. Stain Technol, 40, 333-8. https://doi.org/10.3109/10520296509116440
  15. Jun T, Ming Y, Xin N, et al (2010). Genetic polymorphisms in cytochrome P450 genes are associated with an increased risk of squamous cell carcinoma of the larynx and hypopharynx in a Chinese population. Cancer Genet Cytogenet, 196, 76-82. https://doi.org/10.1016/j.cancergencyto.2009.08.015
  16. Kiruthiga PV, Rajesh Kannan V, Saraswathi C, Karutha Pandian S, Pandima Devi K (2011). CYP1A1 Gene Polymorphisms: Lack of Association with Breast Cancer Susceptibility in the Southern Region (Madurai) of India. Asian Pac J Cancer Prev, 12, 2133-8.
  17. Lee KM, Kang D, Clapper ML, et al (2008). CYP1A1, GSTM1, and GSTT1 polymorphisms, smoking, and lung cancer risk in a pooled analysis among asian populations. Cancer Epidemiol Biomarkers Prev, 17, 1120-6. https://doi.org/10.1158/1055-9965.EPI-07-2786
  18. Lotery AJ (2000). Mutation Analysis of 3 Genes in Patients with Leber Congenital Amaurosis. Arch. Ophthalmol, 118, 538-43.
  19. Masson LF, Sharp L, Cotton SC, et al (2005). Cytochrome P-450 1A1 gene polymorphisms and risk of breast cancer: a HuGE review. Am J Epidemiol, 161, 901. https://doi.org/10.1093/aje/kwi121
  20. Ma Q and Lu AY (2003). Origins of individual's variability in P4501A induction. Chem Res Toxico, 16, 249-60. https://doi.org/10.1021/tx0200919
  21. Mahimkar MB, Bhisey RA (1995). Occupational exposure to bidi tobacco increases chromosomal aberrations in tobacco processors. Mutation Res, 334, 139-44. https://doi.org/10.1016/0165-1161(95)90004-7
  22. Mahimkar MB, Bhisey RA (1995). Occupational exposure to bidi tobacco increases chromosomal aberrations in tobacco processors. MutatRes Apr, 334, 139-44.
  23. Moretti M, dell'Omo M, Villarini M (2007). Primary DNA damage and genetic polymorphisms for CYP1A1, EPHX and GSTM1in workers at a graphite electrode manufacturing plant. BMC Public Health, 7, 270-9. https://doi.org/10.1186/1471-2458-7-270
  24. Masood N, Malik FA, MahJabeen I, Baig RM, Kayani MA (2011). A novel CYP1A1 gene polymorphism and the risk of head and neck cancer in Pakistani population. Afr J Biotechnol, 10, 5273-80.
  25. Okino ST, Pookot D, Li LC, et al (2006). Epigenetic inactivation of the dioxin-responsive cytochrome P4501A1 gene in human prostate cancer. Cancer Res, 66, 7420-8. https://doi.org/10.1158/0008-5472.CAN-06-0504
  26. Orita M (1989). Detection of polymorphism of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci, 86, 2766-70. https://doi.org/10.1073/pnas.86.8.2766
  27. Ranjitsingh AJA, Padmalatha C (1995). Occupational illness of beedi rollers in south India. Env Econ, 13, 875-9.
  28. Sabitha K, Vishnuvardhan MR, Kaiser J (2010). Smoking related risk involved in individuals carrying genetic variants of CYP1A1 gene in head and neck cancer. Cancer Epidemiol, 34, 587-92 https://doi.org/10.1016/j.canep.2010.05.002
  29. Senthilkumar KP, Thirumurugan R (2012). GSTM1 and GSTT1 allele frequencies among various Indian and non-Indian ethnic groups. Asian Pac J Cancer Prev, 13, 6263-7. https://doi.org/10.7314/APJCP.2012.13.12.6263
  30. Sharma A, Pandey A, Sardana S, et al (2012). Genetic polymorphisms of GSTM1 and GSTT1 genes in Delhi and comparison with other Indian and global populations. Asian Pac J Cancer Prev, 13, 5647-52. https://doi.org/10.7314/APJCP.2012.13.11.5647
  31. Shimkhada R, Peabody JW (2003). Tobacco control in india. Bull World Health Organ. 81, 48-52.
  32. Sobti RC, Sharma S, Joshi A, Jindal SK, Janmeja A (2003). CYP1A1 and CYP2D6 polymorphism and risk of lung cancer in a North Indian Population. Biomarkers, 8, 415-28. https://doi.org/10.1080/13547500310001619860
  33. Srinivasulu K (1997). Impact of liberalization on beedi workers. Econ Polit Wkly, 32, 515-7.
  34. Syamala VS, Syamala V, Sheeja VR, et al (2010). Possible risk modification by polymorphisms of estrogen metabolizing genes in familial breast cancer susceptibility in an Indian population. Cancer Invest, 28, 304-11. https://doi.org/10.3109/07357900902744494
  35. Tekpli X, Zienolddiny S, Skaug V, et al (2012). DNA methylation of the CYP1A1 enhancer is associated with smokinginduced genetic alterations in human lung. Int J Cancer, 131, 1509-16. https://doi.org/10.1002/ijc.27421
  36. Turesky RJ, Le Marchand L (2011). Metabolism and biomarkers of heterocyclic aromatic amines in molecular epidemiology studies: lessons learned from aromatic amines. Chem Res Toxicol, 24, 1169-214. https://doi.org/10.1021/tx200135s
  37. Umadevi B, Swarna M, Padmavathi P, Jyothi A, Reddy PP (2003). Cytogenetic effects in workers occupationally exposed to tobacco dust. Genet Toxicol Environ Mutagen, 535, 147-54. https://doi.org/10.1016/S1383-5718(02)00291-7
  38. Yang CX, Matsuo K, Wang ZM, Tajima K (2005). Phase I/II enzyme gene polymorphisms and esophageal cancer risk: A meta-analysis of the literature. World J Gastroenterol, 11, 2531-8. https://doi.org/10.3748/wjg.v11.i17.2531
  39. Yasmin S (2010). Occupational Health Hazards in Women Beedi Rollers in Bihar, India. Bull Environ Contam Toxicol, 85, 87-91. https://doi.org/10.1007/s00128-010-0037-6
  40. Zhang Y, Wise JP, Holford TR, et al (2004). Serum polychlorinated biphenyls, cytochrome P-450 1A1 polymorphisms, and risk of breast cancer in Connecticut women. Am J Epidemiol, 160, 1177. https://doi.org/10.1093/aje/kwh346
  41. Zhou LP, Luan H, Dong XH, et al (2012). Genetic variants of CYP2D6 gene and cancer risk: a HuGE systematic review and meta-analysis. Asian Pac J Cancer Prev, 13, 3165-72. https://doi.org/10.7314/APJCP.2012.13.7.3165

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