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Plasma Post-operative miR-21 Expression in the Prognosis of Gastric Cancers

  • Ma, Guo-Jian (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital) ;
  • Gu, Rong-Min (Department of General Surgery, Jiangsu Cancer Hospital) ;
  • Zhu, Ming (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital) ;
  • Wen, Xu (Department of General Surgery, Jiangsu Cancer Hospital) ;
  • Li, Jin-Tian (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital) ;
  • Zhang, Yuan-Ying (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital) ;
  • Zhang, Xiao-Mei (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital) ;
  • Chen, Sen-Qing (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital)
  • Published : 2013.12.31

Abstract

Tumor-associated microRNAs have been detected in serum or plasma, but whether plasma microRNA-21 (miR-21) could be a potential circulating biomarker for gastric cancer (GC) prognosis in Chinese is still uncertain. Real-time quantitative reverse transcription PCR (qRT-PCR) was employed in this study to compare the relative expression of miR-21 between pre-operative and post-operative paired plasmas from 42 patients with primary GCs. The results showed that the expression levels of miR-21 in the post-operative plasmas were significantly reduced by an average of 18.2 times in all patients when compared to the pre-operative plasmas, and by 22.1 times in the subgroup of patients without family history, while only 1.76 times in the subgroup of patients with a family history. With respect of clinicopathological characteristics, the plasma miR-21 expression was highly associated with differentiation degree and lymph node metastasis rate. The results suggested plasma miR-21 could be a novel potential biomarker for GC prognosis and evaluation of surgery outcomes, especially in patients without a family history.

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