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No Detection of Xenotropic Murine Leukemia Virus-Related Viruses in Prostate Cancer in Sanandaj, West of Iran

  • Khodabandehloo, Mazaher (Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences) ;
  • Hosseini, Weria (Faculty of Allied Medical Sciences, Kurdistan University of Medical Sciences) ;
  • Rahmani, Mohammad-Reza (Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences) ;
  • Rezaee, Mohammad-Ali (Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences) ;
  • Hakhamaneshi, Mohammad-Saied (Cellular and Molecular Research Center, Kurdistan University of Medical Sciences) ;
  • Nikkhoo, Bahram (Department of Pathology, Faculty of Medicine, Kurdistan University of Medical Sciences) ;
  • Jalili, Ali (Cellular and Molecular Research Center, Kurdistan University of Medical Sciences)
  • Published : 2013.11.30

Abstract

Background: Multiple etiologies have been hypothesized for prostate cancer, including genetic defects and infectious agents. A recently reported gamaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) has been reported to be detected in prostate cancer. However, this virus has not been detected in similar groups of patients in other studies. Herein, we sought to detect XMRV in prostate cancers and benign controls in Sanandaj, west of Iran. Materials and Methods: In a case-control study, genomic DNA was extracted from formalin fixed and paraffin embedded prostate tissues from a total of 163 Iranian patients. We developed a conventional and a nested PCR assay using primers targeting to an env specific sequence of XMRV. PCR assays were carried out on 63 prostate cancers and 100 benign prostate hyperplasias. Results: Beta-actin sequences were successfully detected in the DNA extracts from all prostate tissues, confirming DNA extraction integrity. We did not detect XMRV in samples either from prostate cancers or benign prostate hyperplasias using XMRV specific primers. Conclusions: We conclude that in our population XMRV does not play a role in genesis of prostate cancer.

Keywords

XMRV;MLV;prostate cancer;Iran

References

  1. Akbari ME, Hosseini SJ, Rezaee A, et al (2008). Incidence of genitourinary cancers in the Islamic Republic of Iran: a survey in 2005. Asian Pac J Cancer Prev, 9, 549-52.
  2. Aloia AL, Sfanos KS, Isaacs WB, et al (2010). XMRV: a new virus in prostate cancer? Cancer Res, 70, 10028-33. https://doi.org/10.1158/0008-5472.CAN-10-2837
  3. Arnold RS, Makarova NV, Osunkoya AO, et al (2010). XMRV infection in patients with prostate cancer: novel serologic assay and correlation with PCR and FISH. Urol, 75, 755-61. https://doi.org/10.1016/j.urology.2010.01.038
  4. Danielson BP, Ayala GE, Kimata JT (2010). Detection of xenotropic murine leukemia virus-related virus in normal and tumor tissue of patients from the southern United States with prostate cancer is dependent on specific polymerase chain reaction conditions. J Infect Dis, 202, 1470-7. https://doi.org/10.1086/656146
  5. Das Gupta J, Luk KC, Tang N, et al (2012). Absence of XMRV and Closely Related Viruses in Primary Prostate Cancer Tissues Used to Derive the XMRV-Infected Cell Line 22Rv1. PLoS One, 7, 36072. https://doi.org/10.1371/journal.pone.0036072
  6. Erlwein O, Kaye S, McClure MO, et al (2010). Failure to detect the novel retrovirus XMRV in chronic fatigue syndrome. PLoS One 5, 8519. https://doi.org/10.1371/journal.pone.0008519
  7. Fischer N, Hellwinkel O, Schulz C, et al (2008). Prevalence of human gammaretrovirus XMRV in sporadic prostate cancer. J Clinical Virol, 43, 277-83. https://doi.org/10.1016/j.jcv.2008.04.016
  8. Groom HC, Boucherit VC, Makinson K, et al (2010). Absence of xenotropic murine leukaemia virus-related virus in UK patients with chronic fatigue syndrome. Retrovirol, 7, 10. https://doi.org/10.1186/1742-4690-7-10
  9. Groom HC, Warren AY, Neal DE, et al (2012). No evidence for infection of UK prostate cancer patients with XMRV, BK virus, trichomonas vaginalis or human papilloma viruses. PLoS One, 7, 34221. https://doi.org/10.1371/journal.pone.0034221
  10. Hohn O, Krause H, Barbarotto P, et al (2009). Lack of evidence for xenotropic murine leukemia virus-related virus (XMRV) in German prostate cancer patients. Retrovirol, 6, 92. https://doi.org/10.1186/1742-4690-6-92
  11. Hue S, Gray ER, Gall A, et al (2010). Disease-associated XMRV sequences are consistent with laboratory contamination. Retrovirol, 7, 111. https://doi.org/10.1186/1742-4690-7-111
  12. Khan G, Philip PS, Naase M, et al (2012). No evidence for the involvement of XMRV or MCV in the pathogenesis of breast cancer. Br J Cancer, 106, 1166-70. https://doi.org/10.1038/bjc.2012.51
  13. Knox K, Carrigan D, Simmons G, et al (2011). No evidence of murine-like gammaretroviruses in CFS patients previously identified as XMRV-infected. Science, 333, 94-7. https://doi.org/10.1126/science.1204963
  14. Korn K, Reil H, Ensser A, et al (2012). No evidence of XMRV infection in immunocompromised patients and HIV-positive individuals from Germany. Infection, [Epub ahead of print].
  15. Lombardi VC, Ruscetti FW, Das Gupta J, et al (2009). Detection of an infectious retrovirus, XMRV, in blood cells of patients with chronic fatigue syndrome. Science, 326, 585-9. https://doi.org/10.1126/science.1179052
  16. Luczkowiak J, Martinez-Prats L, Sierra O, et al (2012). Lack of the detection of XMRV or polytropic MLV-related sequences in blood cells from HIV-1-infected patients in Spain. J Acquir Immune Defic Syndr, 59, 101-4. https://doi.org/10.1097/QAI.0b013e318238b596
  17. Mendoza R, Silverman RH, Klein EA, et al (2012). No biological evidence of XMRV in blood or prostatic fluid from prostate cancer patients. PLoS One, 7, 36073. https://doi.org/10.1371/journal.pone.0036073
  18. Mi Z, Lu Y, Zhang S, et al (2012). Absence of xenotropic murine leukemia virus-related virus in blood donors in China. Transfusion, 52, 326-31. https://doi.org/10.1111/j.1537-2995.2011.03267.x
  19. Oakes B, Tai AK, Cingoz O, et al (2010). Contamination of human DNA samples with mouse DNA can lead to false detection of XMRV-like sequences. Retrovirol, 7, 109. https://doi.org/10.1186/1742-4690-7-109
  20. Paolucci S, Piralla A, Zanello C, et al (2012). Xenotropic and polytropic murine leukemia virus-related sequences are not detected in the majority of patients with chronic fatigue syndrome. New Microbiol, 35, 341-4.
  21. Reza MA, Fahimeh G, Reza MH (2012). Evaluation of xenotropic murine leukemia virus and its R426Q polymorphism in patients with prostate cancer in Kerman, southeast of Iran. Asian Pac J Cancer Prev, 13, 3669-73. https://doi.org/10.7314/APJCP.2012.13.8.3669
  22. Rezaei SD, Hearps AC, Mills J, et al (2013). No association between XMRV or related gammaretroviruses in Australian prostate cancer patients. Virol J, 10, 20. https://doi.org/10.1186/1743-422X-10-20
  23. Robinson MJ, Erlwein OW, Kaye S, et al (2010). Mouse DNA contamination in human tissue tested for XMRV. Retrovirol, 7, 108. https://doi.org/10.1186/1742-4690-7-108
  24. Robinson MJ, William Tuke P, Erlwein O, et al (2011). No Evidence of XMRV or MuLV Sequences in Prostate Cancer, Diffuse Large B-Cell Lymphoma, or the UK Blood Donor Population. Advances in Virology, 2011, 1-6.
  25. Sakuma T, Hue S, Squillace KA, et al (2011). No evidence of XMRV in prostate cancer cohorts in the Midwestern United States. Retrovirol, 8, 23. https://doi.org/10.1186/1742-4690-8-23
  26. Sato E, Furuta RA, Miyazawa T (2010). An endogenous murine leukemia viral genome contaminant in a commercial RT-PCR kit is amplified using standard primers for XMRV. Retrovirol, 7, 110. https://doi.org/10.1186/1742-4690-7-110
  27. Satterfield BC, Garcia RA, Jia H, et al (2011). Serologic and PCR testing of persons with chronic fatigue syndrome in the United States shows no association with xenotropic or polytropic murine leukemia virus-related viruses. Retrovirol, 8, 12. https://doi.org/10.1186/1742-4690-8-12
  28. Schlaberg R, Choe DJ, Brown KR, et al (2009). XMRV is present in malignant prostatic epithelium and is associated with prostate cancer, especially high-grade tumors. Proc Natl Acad Sci USA, 106, 16351-6. https://doi.org/10.1073/pnas.0906922106
  29. Sfanos KS, Aloia AL, De Marzo AM, et al (2012). XMRV and prostate cancer--a 'final' perspective. Nat Rev Urol, 9, 111-8. https://doi.org/10.1038/nrurol.2011.225
  30. Sfanos KS, Isaacs JT (2011). The "infectious" nature of human prostate cancer: a cautionary note. Oncotarget, 2, 281-3.
  31. Shin CH, Bateman L, Schlaberg R, et al (2011). Absence of XMRV and other MLV-related viruses in patients with Chronic Fatigue Syndrome. J Virol, 85, 7195-202. https://doi.org/10.1128/JVI.00693-11
  32. Silverman RH, Nguyen C, Weight CJ, et al (2010). The human retrovirus XMRV in prostate cancer and chronic fatigue syndrome. Nat Rev Urol, 7, 392-402. https://doi.org/10.1038/nrurol.2010.77
  33. Simmons G, Glynn SA, Holmberg JA, et al (2011). The Blood Xenotropic Murine Leukemia Virus-Related Virus Scientific Research Working Group: mission, progress, and plans. Transfusion, 51, 643-53. https://doi.org/10.1111/j.1537-2995.2011.03063.x
  34. Singh IR, Gorzynski JE, Drobysheva D, et al (2010). Raltegravir is a potent inhibitor of XMRV, a virus implicated in prostate cancer and chronic fatigue syndrome. PLoS One, 5, 9948. https://doi.org/10.1371/journal.pone.0009948
  35. Stieler K, Schindler S, Schlomm T, et al (2011). No detection of XMRV in blood samples and tissue sections from prostate cancer patients in Northern Europe. PLoS One, 6, 25592. https://doi.org/10.1371/journal.pone.0025592
  36. Switzer WM, Jia H, Hohn O, et al (2010). Absence of evidence of xenotropic murine leukemia virus-related virus infection in persons with chronic fatigue syndrome and healthy controls in the United States. Retrovirol, 7, 57. https://doi.org/10.1186/1742-4690-7-57
  37. Switzer WM, Jia H, Zheng H, et al (2011). No association of xenotropic murine leukemia virus-related viruses with prostate cancer. PLoS One, 6, 19065. https://doi.org/10.1371/journal.pone.0019065
  38. Tang N, Frank A, Leckie G, et al (2012). Development of sensitive single-round pol or env RT-PCR assays to screen for XMRV in multiple sample types. J Virol Methods, 179, 127-34. https://doi.org/10.1016/j.jviromet.2011.10.010
  39. Tang S, Hewlett IK (2011). Testing strategies for detection of xenotropic murine leukemia virus-related virus infection. Adv Virol, 2011, 281425.
  40. Tuke PW, Tettmar KI, Tamuri A, et al (2011). PCR master mixes harbour murine DNA sequences. Caveat emptor! PLoS One, 6, 19953. https://doi.org/10.1371/journal.pone.0019953
  41. Urisman A, Molinaro RJ, Fischer N, et al (2006). Identification of a novel Gammaretrovirus in prostate tumors of patients homozygous for R462Q RNASEL variant. PLoS Pathog, 2, 25. https://doi.org/10.1371/journal.ppat.0020025
  42. van Kuppeveld FJ, de Jong AS, Lanke KH, et al (2010). Prevalence of xenotropic murine leukaemia virus-related virus in patients with chronic fatigue syndrome in the Netherlands: retrospective analysis of samples from an established cohort. BMJ, 340, 1018. https://doi.org/10.1136/bmj.c1018
  43. Verhaegh GW, de Jong AS, Smit FP, et al (2011). Prevalence of human xenotropic murine leukemia virus-related gammaretrovirus (XMRV) in dutch prostate cancer patients. Prostate, 71, 415-20. https://doi.org/10.1002/pros.21255
  44. Williams DK, Galvin TA, Gao Y, et al (2013). No evidence of xenotropic murine leukemia virus-related virus transmission by blood transfusion from infected rhesus macaques. J Virol, 87, 2278-86. https://doi.org/10.1128/JVI.02326-12
  45. Zhou Y, Steffen I, Montalvo L, et al (2012). Development and application of a high-throughput microneutralization assay: lack of xenotropic murine leukemia virus-related virus and/or murine leukemia virus detection in blood donors. Transfusion, 52, 332-42. https://doi.org/10.1111/j.1537-2995.2011.03519.x

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