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Oleuropein Induces Apoptosis Via the p53 Pathway in Breast Cancer Cells

  • Hassan, Zeinab Korany (Department of Zoology, College of Science, King Saud University) ;
  • Elamin, Maha Hussein (Department of Zoology, College of Science, King Saud University) ;
  • Omer, Sawsan Ali (Department of Zoology, College of Science, King Saud University) ;
  • Daghestani, Maha Hassan (Department of Zoology, College of Science, King Saud University) ;
  • Al-Olayan, Ebtesam Salah (Department of Zoology, College of Science, King Saud University) ;
  • Elobeid, Mai AbdelRahman (Department of Zoology, College of Science, King Saud University) ;
  • Virk, Promy (Department of Zoology, College of Science, King Saud University)
  • Published : 2013.11.30

Abstract

Background: Breast cancer is a major health problem worldwide. Olive oil induces apoptosis in some cancer cells due to phenolic compounds like oleuropein. Although oleuropein has anticancer activity, the underlying mechanisms of action remain unknown. The study aimed to assess the mechanism of oleuropin-induced breast cancer cell apoptosis. Materials and Methods: p53, Bcl-2 and Bax gene expression was evaluated by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in luminal MCF-7 cells. Results: Oleuropein-induced apoptosis was accompanied by up-regulation of both p53 and Bax gene expression levels and down-regulation in Bcl2. Conclusions: Oleuropein induces apoptosis in breast tumour cells via a p53-dependent pathway mediated by Bax and Bcl2 genes. Therefore, oleuropein may have therapeutic potential in breast cancer patients by inducing apoptosis via activation of the p53 pathway.

Keywords

Apoptotic pathway;Bax gene;breast cancer;Bcl2 gene;oleuropein;p53 gene;semi-quantitative RT-PCR

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