- Volume 14 Issue 11
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Cytokinetic Study of MCF-7 Cells Treated with Commercial and Recombinant Bromelain
- Fouz, Nour (Bioprocess and Molecular Engineering Research Unit, International Islamic University Malaysia) ;
- Amid, Azura (Bioprocess and Molecular Engineering Research Unit, International Islamic University Malaysia) ;
- Hashim, Yumi Zuhanis Has-Yun (Bioprocess and Molecular Engineering Research Unit, International Islamic University Malaysia)
- Published : 2013.11.30
Background: Breast cancer is a leading cause of death in women. The available chemotherapy drugs have been associated with many side effects. Bromelain has novel medicinal qualities including anti-inflammatory, anti-thrombotic, fibrinolytic and anti-cancer functions. Commercially available bromelain is obtained through tedious methods; therefore, recombinant bromelain may provide a cheaper and simpler choice with similar quality. Materials and Methods: This study aimed to assess the effects of commercial and recombinant bromelain on the cytokinetic behavior of MCF-7 breast cancer cells and their potential as therapeutic alternatives in cancer treatment. Cytotoxic activities of commercial and recombinant bromelain were determined using (sulforhodamine) SRB assay. Next, cell viability assays were conducted to determine effects of commercial and recombinant bromelain on MCF-7 cell cytokinetic behavior. Finally, the established growth kinetic data were used to modify a model that predicts the effects of commercial and recombinant bromelain on MCF-7 cells. Results: Commercial and recombinant bromelain exerted strong effects towards decreasing the cell viability of MCF-7 cells with
Bromelain;cell viability;growth kinetics;MCF-7 cells;recombinant
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- vol.33, pp.1, 2016, https://doi.org/10.1002/btpr.2405