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Luteolin, a Bioflavonoid, Attenuates Azoxymethane-Induced Effects on Mitochondrial Enzymes in Balb/c Mice

  • Pandurangan, Ashok Kumar (Department of Biochemistry, University of Madras, Guindy Campus) ;
  • Sadagopan, Suresh Kumar Ananda (Department of Biochemistry, University of Madras, Guindy Campus) ;
  • Dharmalingam, Prakash (Department of Biochemistry, University of Madras, Guindy Campus) ;
  • Ganapasam, Sudhandiran (Department of Biochemistry, University of Madras, Guindy Campus)
  • Published : 2013.11.30

Abstract

Colon cancer (CRC) is a serious health problem throughout the world. Development of novel drugs without side effects for this cancer is crucial. Luteolin (LUT), a bioflavonoid, has many beneficial effects such as antioxidant, anti-inflammatory and anti-proliferative potential. was a potent chemical carcinogen used for the induction of colon cancer. Colon carcinogenesis was initiated by intraperitoneal injection of azoxymethane (AOM) to mice at the dose of 15 mg/body kg weight in Balb/C mice for 3 weeks. Mice were treated with LUT at the dose of 1.2 mg/body kg weight orally. Mitochondrial enzymes such as isocitrate dehydrogenase (ICDH), ${\alpha}$-keto dehydrogenase (${\alpha}$-KDH), succinate dehydrogenase (SDH) and the activities of respiratory chain enzymes NADH dehydrogenase and cytochrome c oxidase were found to be elevated in AOM-treated animals. Treatment with LUT decreased the activities of all the parameters significantly. Hence, LUT might be a potent anticancer agent against colorectal cancer.

Keywords

Colon cancer;luteolin;azoxymethane;chemoprevention;mitochondrial enzymes

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