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Lack of KRAS Gene Mutations in Chronic Myeloid Leukemia in Iran

  • Kooshyar, Mohammad Mahdi (Hematology Department, Cancer Molecular Pathology Research Center) ;
  • Ayatollahi, Hossein (Hematopathology Department, Cancer Molecular Pathology Research Center) ;
  • Keramati, Mohammad Reza (Hematopathology Department, Cancer Molecular Pathology Research Center) ;
  • Sadeghian, Mohammad Hadi (Hematopathology Department, Cancer Molecular Pathology Research Center) ;
  • Miri, Mohsen (Faculty of Medicine, Mashhad University of Medical Sciences) ;
  • Sheikhi, Maryam (Faculty of Medicine, Mashhad University of Medical Sciences)
  • Published : 2013.11.30

Abstract

Background: The single most common proto-oncogene change in human neoplasms is a point mutation in RAS genes. A wide range of variation in frequency of KRAS mutations has been seen in hematologic malignancies. Despite this, RAS roles in leukemogenesis remain unclear. The frequency of KRAS mutations in CML has been reported to be between zero an 10%. Many attempts have been done to develop an anti-RAS drug as a therapeutic target. Materials and Methods: This cross sectional study was performed in Mashhad University of Medical Sciences, Mashhad, Iran from 2010-2012. In 78 CML patients (diagnosed according to WHO 2008 criteria) in chronic or accelerated phases, KRAS mutations in codons 12 and 13 were analyzed using a modified PCR-restriction fragment length polymorphism (RFLP) method. Results: We did not detect any KRAS mutations in this study. Conclusions: KRAS mutations are overall rare in early phase CML and might be secondary events happening late in leukemogenesis cooperating with initial genetic lesions.

Keywords

Leukemia;myelogenous;chronic;BCR-ABL positive;KRAS protein;mutation

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