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miR-126 Suppresses the Proliferation of Cervical Cancer Cells and Alters Cell Sensitivity to the Chemotherapeutic Drug Bleomycin

  • Yu, Qing (Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University) ;
  • Liu, Shan-Ling (Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University) ;
  • Wang, He (Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University) ;
  • Shi, Gang (Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University) ;
  • Yang, Pei (Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University) ;
  • Chen, Xin-Lian (Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University)
  • Published : 2013.11.30

Abstract

In cervical cancer, one of the most common malignant tumors in women worldwide, miR-126 has been reported to exhibit decreased expression. However, its role in cervical cancer cell proliferation and drug sensitivity has remained relatively unexplored. Here, we compared the expression of miR-126 in cervical cancer tissues (n = 20) with that in normal cervical tissue (n = 20) using quantitative RT-PCR. The viability of Siha cervical cancer cells was further measured by MTT assay after transfection with miR-126 mimic (Siha-miR-126 mimic) or microRNA mimic negative control (Siha-miR mimic NC) and after treatment with various concentrations of bleomycin (BLM). IC50s were calculated, and the survival rates (SRs) of Siha cells were calculated. miR-126 expression in cervical cancer tissue was significantly decreased compared with that in normal cervical tissue (P < 0.01). The relative SRs of Siha-miR-126 mimic cells were also significantly decreased compared with those of Siha-miR mimic NC cells at 24-96 h after transfection. The IC50 of BLM in Siha-miR-126 mimic cells ($50.3{\pm}2.02{\mu}g/mL$) was decreased compared with that in Siha-miR mimic NC cells ($70.5{\pm}4.33{\mu}g/mL$) at 48 h after transfection (P < 0.05). Finally, the SRs of Siha-miR-126 mimic cells were significantly lower than those of SihamiR mimic NC cells after cultured in medium containing 40 ${\mu}g/mL$ BLM for 24-96 h (P < 0.05). These results suggest that miR-126 is expressed at low levels in cervical cancer. Upregulation of miR-126 inhibited cervical cancer cell proliferation and enhanced the sensitivity to BLM. Thus, miR-126 may represent a novel approach to cervical cancer treatment.

Keywords

miR-126;cervical cancer;bleomycin;drug sensitivity

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