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Glutathione S-Transferase Expression in Upper Urinary Tract Urothelial Carcinomas: a Taiwan Study

  • Chen, Szu-Han (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Wu, Wen-Jeng (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Tu, Hung-Pin (Department of Public Health and Environmental Medicine, School of Medicine, College of Medicine, Kaohsiung Medical University) ;
  • Li, Wei-Ming (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Huang, Chun-Nung (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Li, Ching-Chia (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Lin, Hui-Hui (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University) ;
  • Ke, Hung-Lung (Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University)
  • Published : 2013.11.30

Abstract

Objectives: Glutathione S-transferase (GST) isoenzymes play important roles in resistance to cell apoptosis and carcinogenesis. We aimed to establish the relationship between GST expression and the prognosis of upper urinary tract urothelial carcinoma (UTT-UC) in Taiwan. Methods: This study retrospectively reviewed 46 patients with pathologically confirmed UUT-UC at Kaohsiung Medical University Hospital. In each patient, expression of GSTT1 and GSTP1 was compared between urothelial carcinoma and normal urothelial cells by Western blotting. Results: GSTP1 expression in the UUT-UC cells was significantly higher than that in normal urothelial cells (1.6 fold, p<0.001). Expression of GSTT1 was significantly associated with the invasiveness of the carcinoma (p=0.006). Conclusions: In UUT-UC, GSTP1 might be a potential tumor marker, whereas high GSTT1 expression could be used as an indicator of cancer progression. This study is the first to demonstrate potential applications of different GST isoenzymes for biomolecular analysis of UUT-UCs in Taiwan.

Keywords

Upper urinary tract;urothelial carcinoma;GSTP;GSTT;protein expression

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