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TIAM2 Enhances Non-small Cell Lung Cancer Cell Invasion and Motility

  • Zhao, Zheng-Yuan (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces) ;
  • Han, Chen-Guang (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces) ;
  • Liu, Jun-Tao (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces) ;
  • Wang, Chang-Lei (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces) ;
  • Wang, Yi (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces) ;
  • Cheng, Li-Ya (Department of General Thoracic Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces)
  • Published : 2013.11.30

Abstract

Background: TIAM2, a Rac guanine nucleotide exchange factor, is closely associated with cell adherence and migration. Here, we aimed to investigate the role of TIAM2 in non-small cell lung cancer (NSCLC) cells. Materials and Methods: A small interference RNA (siRNA) was introduced to silence the expression of TIAM2. Invasion and motility assays were then performed to assess the invasion and motility potential of NSCLC cells. GST-pull down assays were used to detect activation of Rac1. Results: TIAM2 was highly expressed in NSCLC cells. Knockdown of TIAM2 inhibited the invasion and motility, and suppressed activation of Rac1. Further experiments demonstrated that knockdown of TIAM2 could up-regulate the expression of E-cadherin, and down-regulate the expression of MMP-3, Twist and Snail. Conclusions: Our data suggest that TIAM2 can promote invasion and motility of NSCLC cells. Activation of Rac1 and regulation of some EMT/invasion-related genes may be involved in the underlying processes.

Keywords

TIAM2;invasion;motility;non-small cell lung cancer;EMT;Rac1;MMP-3

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