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Effects of PTTG Down-regulation on Proliferation and Metastasis of the SCL-1 Cutaneous Squamous Cell Carcinoma Cell Line

  • Xia, Yong-Hua (Department of Dermatovenereology, the First Affiliated Hospital of Xinxiang Medical University) ;
  • Li, Min (Department of Dermatovenereology, the First Affiliated Hospital of Xinxiang Medical University) ;
  • Fu, Dan-Dan (Department of Dermatovenereology, the First Affiliated Hospital of Xinxiang Medical University) ;
  • Xu, Su-Ling (Department of Oncology, the First Affiliated Hospital of Xinxiang Medical University) ;
  • Li, Zhan-Guo (Department of Dermatovenereology, the First Affiliated Hospital of Xinxiang Medical University) ;
  • Liu, Dong (Department of Dermatovenereology, the First Affiliated Hospital of Xinxiang Medical University) ;
  • Tian, Zhong-Wei (Department of Dermatovenereology, the First Affiliated Hospital of Xinxiang Medical University)
  • Published : 2013.11.30

Abstract

Aims: To study effects of down-regulation of pituitary tumor-transforming gene (PTTG) on proliferation and metastasis ability of the SCL-1 cutaneous squamous cell carcinoma (CSCC) cell line and explore related mechanisms. Methods: SCL-1 cells were divided into 3 groups (untreated, siRNA control and PTTG siRNA). Cell proliferation assays were performed using a CCK-8 kit and proliferation and metastasis ability were analyzed using Boyden chambers. In addition, expression of MMP-2 and MMP-9 was detected by r-time qPCR and Western blotting. Results: Down-regulation of PTTG could markedly inhibit cell proliferation in SCL-1 cells, compared to untreated and control siRNA groups (P < 0.05). Real-time qPCR demonstrated that expression levels of PTTG, MMP-2 and MMP-9 in the PTTG siRNA group were 0.8%, 23.2% and 21.3% of untreated levels. Western blotting revealed that expression of PTTG, MMP-2 and MMP-9 proteins in the PTTG siRNA group was obviously down-regulated. The numbers of migrating cells ($51.38{\pm}4.71$) in the PTTG siRNA group was obviously lower than that in untreated group ($131.33{\pm}6.12$) and the control siRNA group ($127.72{\pm}5.20$) (P < 0.05), suggesting that decrease of proliferation and metastasis ability mediated by PTTG knock-down may be closely correlated with down-regulation of MMP-2 and MMP-9 expression. Conclusion: Inhibition of PTTG expression may be a new target for therapy of CSCC.

Keywords

Cutaneous squamous cell carcinoma;pituitary tumor;transforming gene;proliferation;metastasis

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