Cyclamen Exerts Cytotoxicity in Solid Tumor Cell Lines: a Step Toward New Anticancer Agents?

  • Yildiz, Mustafa (Department of Medical Oncology, Antalya Education and Research Hospital) ;
  • Bozcu, Hakan (Department of Medical Oncology, Akdeniz University Hospital) ;
  • Tokgun, Onur (Department of Medical Biology, Faculty of Arts and Sciences, Pamukkale University) ;
  • Karagur, Ege Riza (Department of Biology, Faculty of Arts and Sciences, Pamukkale University) ;
  • Akyurt, Oktay (Plant Collector) ;
  • Akca, Hakan (Department of Medical Biology, Faculty of Arts and Sciences, Pamukkale University)
  • Published : 2013.10.30


Cyclamen coum is a traditional medicinal plant in the Turkey. Its anticancer properties and whether cyclamen extract induces any cytotoxicity in solid cancer cell lines have not been thoroughly investigated previously. Therefore we examined cytotoxic effects on cervical cells; HeLa and non small cell lung cancer cell, H1299, lines; Cyclamen extract induced cellular death of both HeLa and H1299 cells in a dose dependent manner. We also analyzed the capacity of cyclamen extract to induce apoptosis by the TUNEL method. Here, for the first time we report that the extract of Cyclamen coum, an endemic plant for Turkey, Bulgaria, Georgia and the Middle East can induce cytotoxicity via apoptosis in HeLa and H1299 cells. These results imply that cyclamen extract can be further analyzed to potentially find novel anticancer compounds.


Cyclamen coum;HeLa;H1299;cytotoxicity;apoptosis


  1. Abdulaev FI (2001). Plant-derived agents against cancer, in: Gupta SK, Pharmacology and Therapeutics in the New Millennium, Narosa Publishing House, New Delhi, pp. 345-54.
  2. Altunkeyik H, Gulcemal D, Masullo M, et al (2012). Triterpene saponins from cyclamen hederifoliun. Phytochemistry, 73, 127-33.
  3. Chin YW, Balunas MJ, Chai HB, Kinghorn AD (2006). Drug discovery from natural sources. AAPS J, 8, 239-53.
  4. Arslan S, Ozgun O (2012). Cyclamen Trochopteranthum: Cytotoxic activity and possible adverse interactions including drugs and carcinogens. Chin J Integr Med, 23. [Epub ahead of print].
  5. Bongiovanni G, Palacio L, Eynard A, Cantero JJ, Goleniowski M (2006). In vitro antitumoral activity determination of native plant extracts of the central region of Argentina. Mol Med Chem, 10, 22-3.
  6. Chaouki W, Leger DY, Eljastimi J, Beneytout JL, Hmamouchi M (2010). Antiproliferative effect of extracts from Aristolochia baetica and Origanum compactum on human breast cancer cell line MCF-7. Pharm Biol, 48, 269-74.
  7. Gultekin F, Patat S, Akca H, Akdogan M, Altuntas I (2006). Melatonin can suppress the cytotoxic effects of chlorpyrifos on human hepG2 cell lines. Hum Exp Toxicol, 25, 47-55.
  8. Indap MA, Radhika S, Motiwale L, Rao KVK (2006). Quercetin: antitumor activity and pharmacological manifestations for increased therapeutic gains. Indian J Pharm Sci, 68, 465-69.
  9. Kim JB, Koo HN, Joeng HJ, et al (2005). Introduction of apoptosis by Korean medicine Gagam-whanglyun-haedoktang through activation of capase-3 in human leukemia cell line, HL-60 cells. J Pharmacol Sci, 97, 138-45.
  10. Kinghorn AD, Farnsworth NR, Doel Soejarto D, et al (1999). Novel strategies for the discovery of plantderived anticancer agents. Pure App Chem, 71, 1611-8.
  11. Ozmen A, Bauer S, Gridling M, et al (2009). In vitro anti-neoplastic activity of the ethno-pharmaceutical plant Hypericum adenotrichum spach endemic to Western Turkey. Oncol Rep, 22, 845-52.
  12. Ozmen A, Madlener S, Bauer S, et al (2010). In vitro anti-leukemic activity of the ethno-pharmacological plant Scutellaria orientalis ssp. carica endemic to western Turkey. Phytomedicine, 17, 55-62.
  13. Pezzuto JM (1997). Plant-derived anticancer agents. Biochem Pharmacol, 53, 121-33.
  14. Xia M, Wang D, Wang M, et al (2004). Dracorhodin perchlorate induces apoptosis via activation of caspase and generation of reactive oxygen species. J Pharmacol Sci, 95, 273-83.
  15. Yesson C, Culham A (2006). Phyloclimatic study of cyclamen. BMC Evol Biol, 6, 72.

Cited by

  1. Anti-invasive effect of Cyclamen pseudibericum extract on A549 non-small cell lung carcinoma cells via inhibition of ZEB1 mediated by miR-200c vol.72, pp.3, 2018,