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miR-10b Promotes Migration and Invasion in Nasopharyngeal Carcinoma Cells

  • Sun, Xiao-Jin (Faculty of Pharmacy, Bengbu Medical College) ;
  • Liu, Hao (Faculty of Pharmacy, Bengbu Medical College) ;
  • Zhang, Pei (Faculty of Pharmacy, Bengbu Medical College) ;
  • Zhang, Xu-Dong (Faculty of Pharmacy, Bengbu Medical College) ;
  • Jiang, Zhi-Wen (Faculty of Pharmacy, Bengbu Medical College) ;
  • Jiang, Chen-Chen (Faculty of Pharmacy, Bengbu Medical College)
  • Published : 2013.09.30

Abstract

MicroRNA-10b (miR-10b) has been reported to play an important role in some types of cancer, but the effects and possible mechanisms of action of miR-10b in the metastasis of nasopharyngeal carcinoma cells (NPC) have not been explored. The aim of the present study was to investigate the function of miR-10b in nasopharyngeal carcinoma and to determine the molecular mechanisms underlying its action. The MTT assay was used to assess proliferation of CNE-2Z cells. Wound healing and transwell migration assays were applied to assess cell migration and invasion, while and expression of E-cadherin and MMP-9 were detected using Western blot analysis. Real-time PCR was employed to detect the expression of genes related to migration and invasion and the $2^{-{\Delta}{\Delta}Ct}$ method was used to calculate the degree of expression. MTT assay showed the expression of miR-10b to have no effect on the proliferation of NPC cell lines. The wound healing assay showed that miR-10b mimics promoted the mobility and invasion of NPC cell lines. Inhibitors of miR-10b reduced the ability of NPC cell lines to migrate and invade. In addition, the expression of genes related to migration and invasion, such as E-cadherin, vimentin, and MMP-9, were confirmed to be different in the CNE-2Z NPC cell line transfected with miR-10b mimics and with miR-10b inhibitors. In the present study, miR-10b was found to upregulate the expression of MMP-9 and knockdown of miR-10b was found to significantly downregulate the expression of E-cadherin. On the whole, these results showed that miR-10b plays an important role in the invasion and metastasis of NPC cells.

Keywords

miR-10b;NPC;invasion;metastasis

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