miR-10b Promotes Migration and Invasion in Nasopharyngeal Carcinoma Cells

  • Sun, Xiao-Jin (Faculty of Pharmacy, Bengbu Medical College) ;
  • Liu, Hao (Faculty of Pharmacy, Bengbu Medical College) ;
  • Zhang, Pei (Faculty of Pharmacy, Bengbu Medical College) ;
  • Zhang, Xu-Dong (Faculty of Pharmacy, Bengbu Medical College) ;
  • Jiang, Zhi-Wen (Faculty of Pharmacy, Bengbu Medical College) ;
  • Jiang, Chen-Chen (Faculty of Pharmacy, Bengbu Medical College)
  • Published : 2013.09.30


MicroRNA-10b (miR-10b) has been reported to play an important role in some types of cancer, but the effects and possible mechanisms of action of miR-10b in the metastasis of nasopharyngeal carcinoma cells (NPC) have not been explored. The aim of the present study was to investigate the function of miR-10b in nasopharyngeal carcinoma and to determine the molecular mechanisms underlying its action. The MTT assay was used to assess proliferation of CNE-2Z cells. Wound healing and transwell migration assays were applied to assess cell migration and invasion, while and expression of E-cadherin and MMP-9 were detected using Western blot analysis. Real-time PCR was employed to detect the expression of genes related to migration and invasion and the $2^{-{\Delta}{\Delta}Ct}$ method was used to calculate the degree of expression. MTT assay showed the expression of miR-10b to have no effect on the proliferation of NPC cell lines. The wound healing assay showed that miR-10b mimics promoted the mobility and invasion of NPC cell lines. Inhibitors of miR-10b reduced the ability of NPC cell lines to migrate and invade. In addition, the expression of genes related to migration and invasion, such as E-cadherin, vimentin, and MMP-9, were confirmed to be different in the CNE-2Z NPC cell line transfected with miR-10b mimics and with miR-10b inhibitors. In the present study, miR-10b was found to upregulate the expression of MMP-9 and knockdown of miR-10b was found to significantly downregulate the expression of E-cadherin. On the whole, these results showed that miR-10b plays an important role in the invasion and metastasis of NPC cells.




  1. Baffa R, Fassan M, Volinia S, et al (2009). MicroRNA expression profiling of human metastatic cancers identifies cancer gene targets. J Pathol, 219, 214-21.
  2. Bartel DP (2004). MicroRNAs: genomics, biogenesis, mechanism, and function. Cell, 116, 281-97.
  3. Gee HE, Camps C, Buffa FM, et al (2008). MicroRNA-10b and breast cancer metastasis. Nature, 455, E8-9; author reply E9.
  4. Davis-Dusenbery BN, Hata A (2010). MicroRNA in Cancer: The Involvement of Aberrant MicroRNA Biogenesis Regulatory Pathways. Genes Cancer, 1, 1100-14.
  5. Dawood S (2010). Novel biomarkers of metastatic cancer. Expert Rev Mol Diagn, 10, 581-90.
  6. Edmonds MD, Hurst DR, Vaidya KS, et al (2009). Breast cancer metastasis suppressor 1 coordinately regulates metastasis-associated microRNA expression. Int J Cancer, 125, 1778-85.
  7. Griffiths-Jones S (2004). The microRNA Registry. Nucleic Acids Res, 32, D109-11.
  8. Iorio MV, Croce CM (2009). MicroRNAs in cancer: small molecules with a huge impact. J Clin Oncol, 27, 5848-56.
  9. Li XP, Peng Y, Kung HF, Lin MC (2004). Suppression of Epstein-Barr virus-encoded latent membrane protein-1 by RNA interference inhibits the metastatic potential of nasopharyngeal carcinoma cells. Biochem Biophys Res Commun, 315, 212-18.
  10. Lu J, Getz G (2005). MicroRNA expression profiles classify human cancers. Nature, 435, 834-8.
  11. Luo Z, Zhang L, Jiang C, et al (2011). miR-149 promotes epithelial-mesenchymal transition and invasion in nasopharyngeal carcinoma cells. Zhong Nan Da Xue Xue Bao Yi Xue Ban, 36, 604-9.
  12. Ma L, Teruya-Feldstein J, Weinberg RA, et al (2007). Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature, 449, 682-8.
  13. McDermott AL, Dutt SN, Watkinson JC, et al (2001). The aetiology of nasopharyngeal carcinoma. Clin Otolaryngol Allied Sci, 26, 82-92.
  14. Schuldt A (2007). Micromanaging metastasis. Nat Cell Biol, 9, 1121.
  15. Sossey-Alaoui K, Bialkowska K, Plow EF (2009). The miR200 family of microRNAs regulates WAVE3-dependent cancer cell invasion. J Biol Chem, 284, 33019-29.
  16. Steeg PS (2007). Cancer: micromanagement of metastasis. Nature, 449, 671-3.
  17. Tie J, Fan D (2011). Big roles of microRNAs in tumorigenesis and tumor development. Histol Histopathol, 26, 1353-61.
  18. Wang YS, Wang YH, Xia HP, et al (2012). MicroRNA-214 regulates the acquired resistance to gefitinib via the PTEN/ AKT pathway in EGFR-mutant cell lines. Asian Pac J Cancer Prev, 13, 255-60.
  19. Wei J, Wang F, Kong LY, et al (2013). miR-124 inhibits STAT3 signaling to enhance T cell-mediated immune clearance of glioma. Cancer Res, 73, 3913-26.
  20. Wu W, Yang J, Feng X, et al (2013). MicroRNA-32 (miR-32) regulates phosphatase and tensin homologue (PTEN) expression and promotes growth, migration, and invasion in colorectal carcinoma cells. Mol Cancer, 12, 30.
  21. Yu WM, Hussain SS (2009). Incidence of nasopharyngeal carcinoma in Chinese immigrants, compared with Chinese in China and South East Asia: review. J Laryngol Otol, 123, 1067-74.

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