- Volume 14 Issue 9
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Impact of Co-transfection with Livin and Survivin shRNA Expression Vectors on Biological Behavior of HepG2 Cells
- Xu, Wei (Department of General Surgery, Provincial Hospital Affiliated to Shandong University) ;
- Chang, Hong (Department of General Surgery, Provincial Hospital Affiliated to Shandong University) ;
- Qin, Cheng-Kun (Department of General Surgery, Provincial Hospital Affiliated to Shandong University) ;
- Zhai, Yun-Peng (Department of General Surgery, Provincial Hospital Affiliated to Shandong University)
- Published : 2013.09.30
Objective: To construct short hairpin RNA (shRNA) eukaryotic expression vectors targeting Livin and Survivin genes, and to explore the impact of co-transfection of Livin and Survivin shRNA expression vectors on the biological behavior of HepG2 cells. Methods: shRNA eukaryotic expression vectors pSD11-Livin and pSD11-Survivin were designed and constructed then transfected into HepG2 cells separately or in combination. mRNA and protein expression in transfected cells was assessed by quantitative fluorescence PCR and Western blotting, respectively. Cell proliferation was measured by MTT assay and cell apoptosis by TUNEL assay. Results: The Livin and Survivin shRNA eukaryotic expression vectors were successfully constructed and transfected into HepG2 cells. The relative mRNA expression levels of Livin and Survivin in HepG2 cells co-transfected with pSD11-Livin and pSD11-Survivin were
Livin;Survivin;RNA interference;hepatocellular carcinoma
Supported by : Natural Science Foundation
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