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Antioxidant and Apoptotic Effects of an Aqueous Extract of Urtica dioica on the MCF-7 Human Breast Cancer Cell Line

  • Fattahi, Sadegh (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
  • Ardekani, Ali Motevalizadeh (Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR) ;
  • Zabihi, Ebrahim (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
  • Abedian, Zeinab (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
  • Mostafazadeh, Amrollah (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
  • Pourbagher, Roghayeh (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
  • Akhavan-Niaki, Haleh (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences)
  • Published : 2013.09.30

Abstract

Breast cancer is the most prevalent cancer and one of the leading causes of death among women in the world. Plants and herbs may play an important role in complementary or alternative treatment. The aim of this study was to evaluate the antioxidant and anti-proliferative potential of Urtica dioica. The anti oxidant activity of an aqueous extract of Urtica dioica leaf was measured by MTT assay and the FRAP method while its anti-proliferative activity on the human breast cancer cell line (MCF-7) and fibroblasts isolated from foreskin tissue was evaluated using MTT assay. Mechanisms leading to apoptosis were also investigated at the molecular level by measuring the amount of anti and pro-apoptotic proteins and at the cellular level by studying DNA fragmentation and annexin V staining by flow cytometry. The aqueous extract of Urtica dioica showed antioxidant effects with a correlation coefficient of $r^2$=0.997. Dose-dependent and anti-proliferative effects of the extract were observed only on MCF-7 cells after 72 hrs with an $IC_{50}$ value of 2 mg/ml. This anti proliferative activity was associated with an increase of apoptosis as demonstrated by DNA fragmentation, the appearance of apoptotic cells in flow cytometry analysis and an increase of the amount of calpain 1, calpastatin, caspase 3, caspase 9, Bax and Bcl-2, all proteins involved in the apoptotic pathway. This is the first time such in vitro antiproliferative effect of aqueous extract of Urtica dioica leaf has been described for a breast cancer cell line. Our findings warrant further research on Urtica dioica as a potential chemotherapeutic agent for breast cancer.

Keywords

Breast cancer;Urtica dioica;MCF-7;apoptosis;anti oxidant

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