- Volume 14 Issue 9
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Antioxidant and Apoptotic Effects of an Aqueous Extract of Urtica dioica on the MCF-7 Human Breast Cancer Cell Line
- Fattahi, Sadegh (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
- Ardekani, Ali Motevalizadeh (Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR) ;
- Zabihi, Ebrahim (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
- Abedian, Zeinab (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
- Mostafazadeh, Amrollah (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
- Pourbagher, Roghayeh (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
- Akhavan-Niaki, Haleh (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences)
- Published : 2013.09.30
Breast cancer is the most prevalent cancer and one of the leading causes of death among women in the world. Plants and herbs may play an important role in complementary or alternative treatment. The aim of this study was to evaluate the antioxidant and anti-proliferative potential of Urtica dioica. The anti oxidant activity of an aqueous extract of Urtica dioica leaf was measured by MTT assay and the FRAP method while its anti-proliferative activity on the human breast cancer cell line (MCF-7) and fibroblasts isolated from foreskin tissue was evaluated using MTT assay. Mechanisms leading to apoptosis were also investigated at the molecular level by measuring the amount of anti and pro-apoptotic proteins and at the cellular level by studying DNA fragmentation and annexin V staining by flow cytometry. The aqueous extract of Urtica dioica showed antioxidant effects with a correlation coefficient of
Breast cancer;Urtica dioica;MCF-7;apoptosis;anti oxidant
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