Association of Dietary Intake of Folate, Vitamin B6 and B12 and MTHFR Genotype with Breast Cancer Risk

  • Liu, Ying (The Second Affiliated Hospital of Guangzhou Medical University) ;
  • Zhou, Long-Shu (The Second Affiliated Hospital of Guangzhou Medical University) ;
  • Xu, Xiao-Ming (The Second Affiliated Hospital of Guangzhou Medical University) ;
  • Deng, Liang-Qing (The Second Affiliated Hospital of Guangzhou Medical University) ;
  • Xiao, Qian-Kun (The Second Affiliated Hospital of Guangzhou Medical University)
  • Published : 2013.09.30


Aim: We aimed to investigate the associations of dietary intake of folate, vitamin $B_6$ and $B_{12}$ and MTHFR genotype with breast cancer in a Chinese population. Methods: A matched case-control study was conducted, and 435 patients with newly diagnosed and histologically confirmed breast cancer and 435 controls were collected. The folate intake, vitamin $B_6$ and vitamin $B_{12}$ were calculated, and MTHFR C665T, C677T and A1298C were analyzed by PCR-RFLP. Results: We found vitamin $B_{12}$ was likely to reduce the risk of breast cancer, and MTHFR 665TT was associated with increased risk of breast cancer. Folate intake, vitamin $B_{12}$ intake and variants of MTHFR C677T and MTHFR A1298C demonstrated no association with risk of breast cancer. However, we found patients with low intake of vitamin $B_6$ and MTHFR 665TT genotype had a higher risk of breast cancer (OR=1.87, 95% CI=1.29-2.77), the association being less pronounced among subjects with a moderate intake of vitamin $B_6$ and MTHFR 665TT genotype (OR=1.58, 95% CI=1.03-2.49, P=0.03). Conclusion: Our study indicated that the MTHFR C665T polymorphism and vitamin $B_6$ are associated with risk of breast cancer, which indicated roles for nutrients in developing breast cancer.


Folate;vitamin;MTHFR;polymorphism;breast cancer


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