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MicroRNA-122 Promotes Proliferation, Invasion and Migration of Renal Cell Carcinoma Cells Through the PI3K/Akt Signaling Pathway

  • Lian, Ji-Hu (Department of Urology, China-Japan Union Hospital of Jilin University) ;
  • Wang, Wei-Hua (Department of Urology, China-Japan Union Hospital of Jilin University) ;
  • Wang, Jia-Qiang (Department of Urology, Jilin Province People's Hospital) ;
  • Zhang, Yu-Hong (Department of Urology, Jilin Province People's Hospital) ;
  • Li, Yi (Department of Urology, Jilin Province People's Hospital)
  • Published : 2013.09.30

Abstract

Objective: MicroRNAs (miRNAs) are a small class of non-coding, single-stranded RNAs with a critical role in genesis and maintenance of renal cancer mainly through binding to 3'-untranslated regions (3'UTR) of target mRNAs, which causes a block of translation and/or mRNA degradation. The aim of the present study was to investigate the potential effects of miR-122 in human renal cell carcinomas. Methods: The expression level of miR-122 was quantified by qRT-PCR. MTT, colony formation, invasion and migration assays were used to explore the potential functions of miR-122 in human renal cell carcinoma cells. Results: Cellular growth, invasion and migration in two A498 and 786-O cells were significantly increased after miR-122 transfection. Further experiments demonstrated that overexpression of miR-122 resulted in the increase of phospho-Akt (Ser473) and phospho-mTOR (Ser2448), then activation of mTOR targets, p70S6K and 4E-BP1. Conclusions: The up-regulation of miR-122 may play an important role in the progress of renal cancer through activating PI3K/Akt signal pathway and could be a potential molecular target for anti-cancer therapeutics.

Keywords

miR-122;renal cell carcinoma;invasion;migration

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