- Volume 14 Issue 9
DOI QR Code
Mechanistic Analysis of Taxol-induced Multidrug Resistance in an Ovarian Cancer Cell Line
- Wang, Ning-Ning (Department of Gynecological Oncology Surgery, Jiangsu Cancer Hospital & Institute, Nanjing Medical University) ;
- Zhao, Li-Jun (Department of Gynecological Oncology Surgery, Jiangsu Cancer Hospital & Institute, Nanjing Medical University) ;
- Wu, Li-Nan (Department of Gynecological Oncology Surgery, Jiangsu Cancer Hospital & Institute, Nanjing Medical University) ;
- He, Ming-Feng (Nanjing University of Technology School of Pharmaceutical Science) ;
- Qu, Jun-Wei (Department of Gynecological Oncology Surgery, Jiangsu Cancer Hospital & Institute, Nanjing Medical University) ;
- Zhao, Yi-Bing (Department of Gynecological Oncology Surgery, Jiangsu Cancer Hospital & Institute, Nanjing Medical University) ;
- Zhao, Wan-Zhou (Sino-EU Biomedical Innovation Center (SEBIC), OG Pharma Corporation) ;
- Li, Jie-Shou (Department of General Surgery, Nanjing General Hospital of Nanjing Military Command) ;
- Wang, Jin-Hua (Department of Gynecological Oncology Surgery, Jiangsu Cancer Hospital & Institute, Nanjing Medical University)
- Published : 2013.09.30
Objectives: To establish a taxol-resistant cell line of human ovarian carcinoma (A2780/Taxol) and investigate its biological features. Methods: The drug-resistant cell line (A2780/Taxol) was established by continuous stepwise selection with increasing concentrations of Taxol. Cell morphology was assessed by microscopy and growth curves were generated with in vitro and in vivo tumor xenograft models. With rhodamine123 (Rh123) assays, cell cycle distribution and the apoptotic rate were analyzed by flow cytometry (FCM). Drug resistance-related and signal associated proteins, including P-gp, MRPs, caveolin-1, PKC-
Ovarian carcinoma;Taxol;Multidrug resistance;signaling pathway
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