Analysis of Total Phenolic Content and Antioxidant Activity from Fruits of Vaccinium oldhamii Miq.

정금나무 열매의 총 페놀 함량 및 항산화활성 분석

  • Received : 2013.08.14
  • Accepted : 2013.09.27
  • Published : 2013.12.31


Vaccinium oldhamii Miq. is a Korean native tree, which is deciduous and shrub tree with broad leaf. To develop superior cultivars for edible or medicinal purposes, we evaluated total phenolic content and antioxidant activity. Total phenolic content was measured by folin-denis method and antioxidant activity was measured by DPPH free radical scavenging activity (DPPH method) and reducing power (potassium ferricyanide method). Total phenolic content, DPPH free radical scavenging activity (at 400 ppm) and reducing power (at 700 ppm) were 15.8~19.9 mg/g, 69.1~91.4% and 0.56~0.96, respectively. A linear correlation was shown between DPPH-radical scavenging activity and reducing power (r=0969).


DPPH free radical scavenging activity;reducing power;total phenolic content;Vaccinium oldhamii


  1. Branen, A.L. 1975. Toxicological and biochemistry of butylated hydroxyanisol and butylated hydroxytoluene. Journal of the American Oil Chemists' Society 52: 59-63.
  2. Ames, B.N. 1998. Micronutrients prevent cancer and delay aging. Toxicology letters 102: 5-18.
  3. Chae, J.W., Jo, B.S., Joo, S.H., Ahn, D.H., Chun, S.S., and Cho, Y.J. 2012. Biological and antimicrobial activity of Vaccinium oldhami fruit. Journal of the Korean Society of Food Science and Nutrition 41:1-6.
  4. Finkel, T. and Holbrook, N. 2000. Oxidants, oxidative stress and biology of aging. Nature 408: 239-247.
  5. Guerrero, J.C., Ciampi, L.P., Castilla, A.C., Medel, F.S., Schalchli, H.S., Hormazabal, E.U., Bensch, E.T., and Alberdi, M.L. 2010. Antioxidant capacity, anthocyanins, and total phenols of wild and cultivated berries in Chile. Chilean Journal of Agricultural Research 70: 537-544.
  6. Gulcin, I., Oktay, M., Kufrevioglu, O.I., and Aslan, N.A. 2002. Determination of antioxidant activity of lichen Chtraria islandica (L.) Ach. Journal of Ethnopharmacology 79: 325-329.
  7. Hasan, S.M., Hossain, M.M., Faruque, A., Mazumder, M.E.H., Rana, M.S., Akter, R., and Alam, M.A. 2008. Comparison of antioxidant potential of different fractions of Commelina benghalensis Linn. Bangladesh Journal of Life Sciences 20: 9-16.
  8. Hirotoshi, T., Hisato, K., Ryoko, K.T., Kazuo, N., Masao, Y., Haruki, K., and Chizuko, Y. 2013. Antioxidant activities and anti-cancer cell proliferation properties of Natsuhaze (Vaccinium oldhamii Miq.), Shashanbo (V. bracteatum Thunb.) and Blueberry cultivars. Plants 2: 57-71.
  9. Huang, D.J., Ou, B.X., and Prior, R.L. 2005. The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry 53: 1841-1856.
  10. Jeon, M.H. and Lee, W.J. 2011. Characteristics of blueberry added Makgeolli. Journal of the Korean Society of Food Science and Nutrition 40: 444-449.
  11. Jeong, C.H., Choi, S.G., and Heo, H.J. 2008. Analysis of nutritional compositions and antioxidative activities of Korean commercial blueberry and raspberry. Journal of the Korean Society of Food Science and Nutrition 37: 1375-1381.
  12. Kang. H.H. 2009. Determination of biological activities of Korean berries and their anthocyanin identification. PhD Diss., Gyeongsang National Univ., Korea.
  13. Kim, K.H., Kim, N.Y., Kim, S.H., Han, I.A. and Yook, H.S. 2012. Study on antioxidant effects of fractional extracts from Ligularia stenocephala Leaves. Journal of the Korean Society of Food Science and Nutrition 41: 1220-1225.
  14. Kim, M.H., Lee, Y.J., Kim, D.S., and Kim, D.H. 2003. Quality characteristics of fruits dressing. Korean Journal of Food and Cookery Science 19:165-173.
  15. Kim, S.J., Lee, J.J., Lee, S.I., Park, H.W., Lee, J.H., Park, J.S., Kim, S.H., Baek, N.I., Kwon, Y.E., Yang, J.H., Chae, B.S., Lim, J.P., Shin, T.Y., Jeaon, H., Eun, J.S., and Kim, D.K. 2007. Lignan Glycosides of Vaccinium oldhami Miquel. Korean Journal of Pharmacognosy 38: 296-298.
  16. Kim, T.C., Bae, K.S., Kim, I.K., and Chun, H.J. 2005. Antioxidative activities of solvent extracts from blueberry. Korean Journal of Oriental Physiology & Pathology 19: 179-183.
  17. Kim, T.J. 1996. Korean resources plants III. Seoul National Univ. Korea. pp. 230.
  18. Kwon, J.W., Lee, E.J., Kim, Y.C., Lee, H.S., and Kwon, T.O. 2008. Screening of antioxidant activity from medicinal plant extracts. Korean Journal of Pharmacognosy 39: 155-163.
  19. Lee, J.H., Lee, K.T., Yang, J.H., Baek, N.I., and Kim, D.K. 2004. Acetylcholinesterase inhibitors from the Twigs of Vaccinium oldhami Miquel. Archives of Pharmacal Research 27: 53-56.
  20. Lee, W.G. and Lee, J.A. 2012. Quality characteristics of yogurt dressing prepared with Blueberry juice. The Korean Journal of Culinary Research 18: 255-265.
  21. Lee, Y.N. 1997. Flora of Korea. Kyo-Hak Publoshig Co, Seoul, Korea. pp. 590-592.
  22. Oh, S. and Koh, S.C. 2009. Screening of antioxidative activity and ${\alpha}$-amylase inhibitory activity in angiosperm plants native to Jeju island. Korean Journal of Plant Resources 22: 71-77.
  23. Osawa, T. 1994. Novel natural antioxidant for utilization in food and biological system. In Postharvest Biochemistry of Plant Food Material in the Tropics. Uritani I, Garcia VV, Mendoza EM, eds. Japan Scientific Societies Press, Tokyo, Japan. pp. 241-251.
  24. Pokorny, J. 2007. Are natural antioxidant better - and safer -than synthetic antioxidnats? European Journal of Lipid Science and Technology 109: 629-642.
  25. Rahman, M.A.A. and Moon, S.S. 2007. Antioxidant polyphenol glycosides from the plant Draba nemorosa. Bulletin of the Korean Chemical Society 28: 827-831.
  26. Rice-Evans, C., Miller, N. and Paganga, G. 1996. Structureantioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine 20: 933-956.
  27. Salehi, P., Asghari, B., Asghari, M.A., Dehghan, H., and Ghazi, I. 2013. ${\alpha}$-Glucosidase and a-amylase inhibitory effect and antioxidant activity of ten plant extracts traditionally used in Iran for diabetes. Journal of Medicinal Plants Research 7: 257-266.
  28. Shahidi, F. and Ghazi, J.P.D. 1992. Phenolic antioxidants. Critical Reviews in Food Science and Nutrition 32: 67-103.
  29. Shin, S.W., Lee, J.H., and Soo, B.K. 2012. Antioxidant and antimicrobial activities of Xanthium sibiricum. Korean Journal of Plant Resources 25:372-378.
  30. Sofowara, A. 1993. Medicinal plants and Traditional medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria. pp. 289.
  31. Valentova, K., Ulrichova, J., Cvak, L. and Simanek, V. 2007. Cytoprotective effect of a bilberry extract against oxidative damage of rat hepatocytes. Food Chemistry 101: 912-917.
  32. Velioglu, Y.S., Mazza, G., Gao, L., and Oomah, B.D. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. Journal of Agricultural and Food Chemistry 26: 4113-4117.
  33. Yildirim, A., Mavi, A., Oktay, M., Kara, A.A., Algur, O.F., and Bilaloglu, V. 2000. Comparison of antioxidant and antimicrobial activities of tilia (Tilia argenta DesfEx DC), sage (Salvia triloba L.) and black tea (Camellia sinensis) extracts. Journal of Agricultural and Food Chemistry 48: 5030-5034.
  34. Yoo, M.Y., Park, S.H., Kang, Y.M., and Yang, J.Y. 2005. Characterization of antioxidants extracted from leaves of Sanjook (Sasa voreails var. chiisanensis). Journal of Life Science 15: 796-801.

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