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Identification and Quantification of Glucosinolates in Rapeseed (Brassica napus L.) Sprouts Cultivated under Dark and Light Conditions

  • Lee, Min-Ki (Department of Bio-environmental Chemistry, Chungnam National University) ;
  • Arasu, Mariadhas Valan (Department of Bio-environmental Chemistry, Chungnam National University) ;
  • Chun, Jin-Hyuk (Department of Bio-environmental Chemistry, Chungnam National University) ;
  • Seo, Jeong Min (Department of Bio-environmental Chemistry, Chungnam National University) ;
  • Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University) ;
  • Hong, Soon-Taek (Department of Food Science and Technology, Chungnam National University) ;
  • Kim, In Ho (Department of Animal Resource & Science, Dankook University) ;
  • Lee, Yong-Hwa (Bio-Energy Crop Center, National Institute of Food Science, Rural Development Administration) ;
  • Jang, Young-Seok (Bio-Energy Crop Center, National Institute of Food Science, Rural Development Administration) ;
  • Kim, Sun-Ju (Department of Bio-environmental Chemistry, Chungnam National University)
  • Received : 2013.08.28
  • Accepted : 2013.09.17
  • Published : 2013.12.31

Abstract

BACKGROUND: This study was performed for the identification and quantification of glucosinolate (GSL) contents in seven varieties of rapeseed (Brassica napus L.) sprouts cultivated under dark and light conditions. METHODS AND RESULTS: Crude glucosinolates (GSLs) were desulfated by treating with aryl sulfatase and purified using diethylaminoethyl sepharose (DEAE) anion exchange column. Individual GSLs were quantified using high-performance liquid chromatography (HPLC) with electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Eleven GSLs including six aliphatic (progoitrin, sinigrin, glucoalyssin, gluconapoleiferin, gluconapin, and glucobrassicanapin), four indolyl (4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin) and one aromatic (gluconasturtiin) were identified based on the fragmentation patterns of MS spectrum. Aliphatic GSLs were noted as the predominant group with average 85.2% of the total contents. The most abundant GSLs were progoitrin which was ranged at $8.14-118.68{\mu}mol/g$ dry weight (DW). The highest total GSL amounts were documented in 'Hanra' ($146.02{\mu}mol/g$ DW) under light condition and 'Mokpo No. 68' ($86.67{\mu}mol/g$ DW) in dark condition, whereas the lowest was in 'Tamra' (30.13 and $14.50{\mu}mol/g$ DW) in both conditions. The sum of aliphatic GSLs attributed > 80% in all varieties, except 'Tamra' (67.7% and 64.9% in dark and light conditions, respectively) in the total GSL accumulation. Indolyl GSLs were ranged $2.41-15.73{\mu}mol/g$ DW, accounted 2.78-33.6% of the total GSLs in rapeseed varieties. CONCLUSION(S): These results provide valuable information regarding potential beneficial GSL contents individually. This study attempts to contribute to knowledge of the nutritional properties of the different varieties of rapeseed plants. These results may be useful for the evaluation of dietary information.

Keywords

Brassica napus L.;Glucosinolates;LC-ESI-MS/MS;Light conditions

Acknowledgement

Grant : Cooperative Research Program for Agriculture Science & Technology Development

Supported by : Rural Development Administration

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