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DOSE AND DOSE RATE EFFECTS OF IRRADIATION ON BLOOD COUNT AND CYTOKINE LEVEL IN BALB/c MICE

  • Son, Yeonghoon (Dongnam institute of radiological & Medical Sciences (DIRAMS)) ;
  • Jung, Dong Hyuk (Dongnam institute of radiological & Medical Sciences (DIRAMS)) ;
  • Kim, Sung Dae (Dongnam institute of radiological & Medical Sciences (DIRAMS)) ;
  • Lee, Chang Geun (Dongnam institute of radiological & Medical Sciences (DIRAMS)) ;
  • Yang, Kwangmo (Dongnam institute of radiological & Medical Sciences (DIRAMS)) ;
  • Kim, Joong Sun (Dongnam institute of radiological & Medical Sciences (DIRAMS))
  • Received : 2013.11.05
  • Accepted : 2013.11.29
  • Published : 2013.12.30

Abstract

The biological effects of radiation are dependent on the dose rate and dose of radiation. In this study, effects of dose and dose rate using whole body radiation on plasma cytokines and blood count from male BALB/c mice were evaluated. We examined the blood and cytokine changes in mice exposed to a low (3.49m Gy $h^{-1}$) and high (2.6 Gy $min^{-1}$) dose rate of radiation at a total dose of 0.5 and 2 Gy, respectively. Blood from mice exposed to radiation were evaluated using cytokine assays and complete blood count. Peripheral lymphocytes and neutrophils decreased in a dose dependent manner following high dose rate radiation. The peripheral lymphocytes population remained unchanged following low dose rate radiation; however, the neutrophils population increased after radiation. The sera from these mice exhibited elevated levels of flt3 ligand and granulocyte-colony-stimulating factor (G-CSF), after high/low dose rate radiation. These results suggest that low-dose-rate radiation does not induce blood damage, which was unlike high-dose-rate radiation treatment; low-dose-rate radiation exposure activated the hematopoiesis through the increase of flt3 ligand and G-CSF.

References

  1. Brenner DJ, Doll R, Goodhead DT, Hall EJ, Land CE, Little JB, Lubin JH, Preston DL, Preston RJ, Puskin JS, Ron E, Sachs RK, Samet JM, Setlow RB, Zaider M. Cancer risks attributable to low doses of ionizing radiation: assessing what we really know. Proc. Natl. Acad. Sci. USA. 2003;100:13761-13766 . https://doi.org/10.1073/pnas.2235592100
  2. Pandey R, Shankar BS, Sharma D, Sainis KB. Low dose radiation induced immunomodulation: effect on macrophages and CD8+ T cells. Int. J. Radiat. Bio. 2005;81:801-812. https://doi.org/10.1080/09553000500531886
  3. Ren H, Shen J, Tomiyama-Miyaji C, Watanabe M, Kainuma E, Inoue M, Kuwano Y, Abo T. Augmentation of innate immunity by low-dose irradiation. Cell Immunol. 2006;244:50-56. https://doi.org/10.1016/j.cellimm.2007.02.009
  4. Kim SD, Gong EJ, Bae M, Yang K, Kim JS. Bioassay in BALB/c mice exposed to low dose rate radiation. Journal of Radiation Protection. 2012;37(3):159-166. https://doi.org/10.14407/jrp.2012.37.3.159
  5. Lee DK, Kang HJ. Effects on heamopoietic cells and chromosomal DNA of leukocytes in mice irradiated with low dose of X-ray I. Changes of leukocyte, lymphocyte, thrombocyte and erythrocyte number. Kor. J. Vet. Phbl. Hlth. 2002;26:143-152.
  6. Eric H. Dose response relationship for normal tissues. In. Radiobiology for the radiologist 2nd ed. Philadelphia, PA; Lippincott Co.
  7. Fliedner TM, Tibken B, Hofer EP, Paul W. Stem cell responses after radiation exposure: A key to the evaluation and prediction of its effects. Health Phys. 1996;70:787-797. https://doi.org/10.1097/00004032-199606000-00002
  8. Kim CS, Kim JK, Nam SY, Yang KH, Jeong M, Kim HS, Kim CS, Jin YW, Kim J. Low-dose radiation stimulates the proliferation of normal human lung fibroblasts via a transient activation of Raf and Akt. Mol. Cells. 2007;24(3):424-430.
  9. Tsiperson VP, Soloviev MY. The impact of chronic radioactive stress on the immuno-physiological condition of small mammals. Sci. Total Environ. 1997;203(2):105-113. https://doi.org/10.1016/S0048-9697(97)00138-1
  10. Lopez AF, Hercus TR, Ekert P, Littler DR, Guthridge M, Thomas D, Ramshaw HS, Stomski F, Perugini M, D'Andrea R, Grimbaldeston M, Parker MW. Molecular basis of cytokine receptor activation. IUBMB Life. 2010;62(7):509-518. https://doi.org/10.1002/iub.350
  11. Drouet M, Herodin F. Radiation victim management and the haematologist in the future: time to revisit therapeutic guidelines?. Int. J. Radiat. Biol. 2010;86(8):636-648. https://doi.org/10.3109/09553001003789604
  12. Ashihara E, Shimazaki C, Sudo Y, Kikuta T, Hirai H, Sumikuma T, Yamagata N, Goto H, Inaba T, Fujita N, Nakagawa M. FLT-3 ligand mobilizes hematopoietic primitive and committed progenitor cells into blood in mice. Eur. J. Haematol. 1998;60(2):86-92.
  13. Kim JS, Yang M, Jang H, Oui H, Kim SH, Shin T, Jang WS, Lee SS, Moon C. Granulocyte-colony stimulating factor ameliorates irradiation-induced suppression of hippocampal neurogenesis in adult mice. Neurosci. Lett. 2010;486(1):43-46. https://doi.org/10.1016/j.neulet.2010.09.041
  14. Kim J, Lee S, Jeon B, Jang W, Moon C, Kim S. Protection of spermatogenesis against gamma ray-induced damage by granulocyte colony-stimulating factor in mice. Andrologia. 2011;43(2):87-93. https://doi.org/10.1111/j.1439-0272.2009.01023.x
  15. Du N, Feng K, Luo C, Li L, Bai C, Pei X. Radioprotective effect of FLT3 ligand expression regulated by Egr-1 regulated element on radiation injury of SCID mice. Exp. Hemato. 2003;31(3):191-196. https://doi.org/10.1016/S0301-472X(02)01082-2
  16. Prat M, Demarquay C, Frick J, Dudoignon N, Thierry D, Bertho JM. Use of flt3 ligand to evaluate residual hematopoiesis after heterogeneous irradiation in mice. Radiat. Res. 2006;166(3):504-511. https://doi.org/10.1667/RR0568.1
  17. Grimbaldeston MA, Nakae S, Kalesnikoff J, Tsai M, Galli SJ. Mast cell-derived interleukin 10 limits skin pathology in contact dermatitis and chronic irradiation with ultraviolet B. Nat. Immunol. 2007;8(10):1095-1104. https://doi.org/10.1038/ni1503
  18. Lopes CO, Callera F. Three-dimensional conformal radiotherapy in prostate cancer patients: rise in interleukin 6 (IL-6) but not IL-2, IL-4, IL-5, tumor necrosis factor-alpha, MIP-1-alpha, and LIF levels. Int. J. Radiation Oncology Biol. Phys. 2012;82(4):1385-1388. https://doi.org/10.1016/j.ijrobp.2011.04.040
  19. Deorukhkar A, Krishnan S. Targeting inflammation pathway for tumor radiosensitization. Biochemical Pharmacology. 2010;80(12):1904-1914. https://doi.org/10.1016/j.bcp.2010.06.039
  20. Patchen ML, Fischer R, MacVittie TJ. Effects of combined administration of interleukin-6 and granulocyte colony-stimulating factor on recovery from radiation-induced hemopoietic aplasia. Exp. Hematol. 1993;21(2):338-344.

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