Mixture of Edwardsiella tarda specific Bacteriophage and Bacillus subtilis KM-1enhanced bactericidal activity against Edwardsiella tarda

Edwardsiella tarda의 특이 Bacteriophage와 Bacillus subtilis KM-1혼합액이 Edwardsiella tarda 에 미치는 항균효과

  • Baek, Min Suk (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Hwang, Yo Sep (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Sanghoon (Department of Aquatic Life Medicine, Kunsan National University)
  • 백민석 (군산대학교 해양과학대학 수산생명의학과) ;
  • 황요셉 (군산대학교 해양과학대학 수산생명의학과) ;
  • 최상훈 (군산대학교 해양과학대학 수산생명의학과)
  • Received : 2013.10.10
  • Accepted : 2013.11.19
  • Published : 2013.12.31


The present study was performed to investigate an antibacterial activity of specific bacteriophage (phage) and Bacillus subtilis KM-1 (B. subtilis) mixture against Edwardsiella tarda (E. tarda). An appropriate number of phage showing the most effective antibacterial activity was $2{\times}10^5$ PFU/ml with $1{\times}10^7$ CFU/ml of B. subtilis 36 h post incubation. On the other hand, B. subtilis showed a dose dependant manner in inducing antibacterial activity in the presence of phage ($2{\times}10^5$ PFU/ml). The phage and B. subtilis mixture showed higher antibacterial activity against E. tarda than phage or B. subtilis only. These results suggest that the phage and B. subtilis mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.


Supported by : 군산대학교


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