Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Arthrospira platensis Mutants Containing High Lipid Content by Electron Beam Irradiation and Analysis of Its Fatty Acid Composition

전자빔 조사에 의해 지질 함량이 증대된 Arthrospira platensis 변이주 분리 및 지방산 분석

  • Choi, Soo-Jeong (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University) ;
  • Kim, Young-Hwa (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University) ;
  • Kim, Andre (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
  • 최수정 (신라대학교 의생명과학대학 생명공학과) ;
  • 김영화 (신라대학교 의생명과학대학 제약공학과) ;
  • 김안드레 (신라대학교 의생명과학대학 제약공학과) ;
  • 이재화 (신라대학교 의생명과학대학 생명공학과)
  • Received : 2013.07.19
  • Accepted : 2013.08.22
  • Published : 2013.12.10

Abstract

Arthrospira platensis (A. platensis) is an economically important microalgae because it has carbohydrates, lipids, proteins and a number of phytochemicals. It is also a valuable source used in the production of biodiesel and functional foods. In this study, A. platensis was exposed to electron beam irradation (240 kGy) and induced random mutagenesis for strain improvement. Several mutants were obtained, and the resulting mutant was designated as EB29. The growth rate and chlorophyll content of EB29 was similar to those of wild type. However, the lipid content of EB29 was increased seven-fold compared to that of wild type when comparing the nile red fluorescent intensity. Semi-quantitative analysis of EB29 using the calibration plot of standard lipid, triolein, represented $78.6{\mu}g/mL$, which increased 2 times compared to wild type ($41.4{\mu}g/mL$). When analyzing the fatty acid profile of EB29, polyunsaturated fatty acids (PUFAs), such as gamma-linolenic acid (GLA) in EB29 increased about six-fold. Moreover, fatty acids affecting the quality of biodiesel increased compared to that of wild type. Thus, electron beam could be used for the strain improvement of microalgae in order to accumulate PUFAs and alteration of fatty acid profile for biodiesel.

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