DOI QR코드

DOI QR Code

The Effect of Ulmus Root-bark Dressing in Fibroblast Growth Factor and Vascular Endothelial Growth Factor of Induced Pressure Ulcer in Rats

느릅나무 근피드레싱이 쥐에 유발된 욕창의 섬유아세포성장인자와 혈관내피성장인자에 미치는 효과

Na, Yeon Kyung
나연경

  • Received : 2013.10.25
  • Accepted : 2013.11.12
  • Published : 2013.11.30

Abstract

Purpose: The purpose of this study was to investigate the effect of Ulmus root-bark dressing in fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) of induced pressure ulcers in rats. Methods: 54 male Sprague-Dawley rats were used and randomly divided into 2 groups. The rats were anesthetized and pressure ulcers were induced at 140 mmHg for three hours, using a personally-designed pressing apparatus. Ulmus dressing was applied in the experimental group (n=27) and saline gauze dressing in the control group (n=27). Each of the dressings was changed every other day, and after a month, the wounds were examined by microscopy biweekly for 20 weeks. Results: After 4 weeks, the epidermis of the wounds was recovered, but inflammatory infiltration of the dermis was remained. After 6 weeks, inflammatory cells were diminished and the number of capillaries was decreased. Examined by immunofluorescence staining, the FGF positive cells in the experimental group changed negatively after 18 weeks, but the control group still existed even after 20 weeks. VEGF positive cells in the experimental group also changed negatively after 20 weeks, but the control group still existed. Conclusion: The findings of this study demonstrate that Ulmus dressing is effective in minimizing scar formation and inflammatory reaction by decreasing FGF and VEGF in the terminal phase of wound healing.

Keywords

Pressure ulcer;Dressing;Fibroblast Growth Factor;Vascular Endothelial Growth Factor;Wound healing

References

  1. Corral, C. J., Siddiqui, A., Wu, L., Farrell, C. L., Lyons, D., & Musloe, T. A. (1999). Vascular endothelial growth factor is more important than basic fibroblastic growth factor during ischemic wound healing. Archives of Surgery, 134(2), 200-205. http://dx.doi.org/10.1001/archsurg.134.2.200 https://doi.org/10.1001/archsurg.134.2.200
  2. Dai, Y. L., Pan, Y. Y., Sun, Y., Cui, F. F., Zhang, L., Xiao, J., et al. (2012). Expression of vascular endothelial growth factor and basic fibroblast growth factor in the late stage of pressure ulcer. Zhonghua Shao Shang Za Zhi, 28(5), 363-366.
  3. Fujiwara, M., Muragaki, Y., & Ooshima, A. (2005). Upregulation of transforming growth factor-beta1 and vascular endothelial growth factor in cultured keloid fibroblasts: relevance to angiogenic activity. Achives of Dermatological Research, 297(4), 161-169. http://dx.doi.org/10.1007/s00403-005-0596-2 https://doi.org/10.1007/s00403-005-0596-2
  4. Gabriel, A., Mussman, J., Rosenberg, Z. L., & Torre, J. I. (2003). Wound healing, Growth factor. Retrieved August 22, 2011, from http://emedicine.medscape.com/article/1298196-overview
  5. Gale, N., W., & Yancopoulos, G. D. (1999). Growth factors acting via endothelial cell-specific receptor tyrosine kinases: VEGFs, angiopoietins, and ephrins in vascular development. Genes & Development, 13(9), 1055-1066. http://dx.doi.org/10.1101/gad.13.9.1055 https://doi.org/10.1101/gad.13.9.1055
  6. Greenhalgh, D. G., Sprugel, K. H., Murray, M. J., & Ross, R. (1990). PDGF and FGF stimulate wound healing in the genetically diabetic mouse. American Journal of Pathology, 136(6), 1235-1246.
  7. Bao, P., Kodra, A., Tomic-Canic, M., Golinko, M. S., Ehrlich, P., & Brem, H. (2009). The role of vascular endothelial growth factor in wound healing. Journal of Surgical Research, 153(2), 347-358. http://dx.doi.org/10.1016/j.jss.2008.04.023 https://doi.org/10.1016/j.jss.2008.04.023
  8. Cho, S. K., Lee, S. G., & Kim, C. J. (1996). Anti-inflammatory and analgesic activities of water extract of root bark of ulmus parvifolia. Korean Journal of Pharmacognosy, 27(3), 274-281.
  9. Lim, A. K., Kim, K. S., Park, S. J., Hong, J. H., Choi, H. J., & Kim, D. I. (2010). Healing effects of ginsenoside Rg1 on experimental open wound in rat. Journal of Korean Society of Food Science Nutrition, 39(10), 1452-1458. http://dx.doi.org/10.3746/jkfn.2010.39.10.1452 https://doi.org/10.3746/jkfn.2010.39.10.1452
  10. Lin, Z. Q., Kondo, T., Ishida, Y., Takayasu, T., & Mukaida, N. (2003). Essential involvement of IL-6 in the skin wound-healing process as evidenced by delayed wound healing in IL-6-deficient mice. Journal of Leukocyte Biology, 73(6), 713-721. http://dx.doi.org/10.1189/jlb.0802397 https://doi.org/10.1189/jlb.0802397
  11. Mandriota, S. J., & Pepper, M. S. (1997). Vascular endothelial growth factor-induced in vitro angiogenesis and plasminogen activator expression are dependent on endogenous basic fibroblast growth factor. Journal of Cell Science, 110(18), 2293-2302.
  12. Na, Y. K., & Hong, H. S. (2006). The effects of the ulmus root-bark dressing in tissue regeneration of induced pressure ulcer in rats. Journal of Korean Academy Nursing, 36(6), 523-531. https://doi.org/10.4040/jkan.2006.36.3.523
  13. Nam, M. H., & Lim, J. H. (2012). Analysis on the situation of inpatients with pressure ulcer by patient safety indicators. The Journal of Digital Policy and Management, 10(3), 197-205.
  14. R&D System Inc. (2002, January 1). Cytokines in Wound Healing. Retrieved August 10, 2013, from http://www.rndsystems.com/mini_review_detail_objectname_MR02_CytokineWoundHealing.aspx
  15. Robson, M. C., Philips, L. G., Lawrence, W. T., Bishop, J. B., Yongerman, J. S., Hayward, P. G., et al. (1992). The safety and effect of topically applied recombinant basic fibroblast growth factor on the healing of chronic pressure sores. Annals of Surgery, 216(4), 401-408. http://dx.doi.org/10.1097/00000658-199210000-00002 https://doi.org/10.1097/00000658-199210000-00002
  16. Ryu, H. S., Park, C. S., Kim, I. A., Kwon, Y. D., & Kang, S. W. (2008). Use of home nursing therapy and need of home care equipments. Journal of Korean Academy Community Health Nursing, 19(2), 157-166.
  17. Seghezzi, G., Patel, S., Ren, C. J., Gualandris, A., Pintucci, G., Robbins, E. S., et al. (1998). Fibroblast growth factor-2 (FGF-2) induces vascular endothelial growth factor (VEGF) expression in the endothelial cells of forming capillaries: An autocrine mechanism contributing to angiogenesis. Journal of Cell Biology, 141(7), 1659-1673. http://dx.doi.org/10.1083/jcb.141.7.1659 https://doi.org/10.1083/jcb.141.7.1659
  18. Stravi, G. T., Zachary, I. C., Baskerville, P. A., Martin, J. F., & Erusalimsky, J. D. (1995). Basic fibroblast growth factor upregulates the expression of vascular endothelial growth factor in vascular smooth muscle cells. Synergistic interaction with hypoxia. Circulation, 92(1), 11-14. http://dx.doi.org/10.1161/01.CIR.92.1.11 https://doi.org/10.1161/01.CIR.92.1.11
  19. Takayama, M., Kuramoto, Y., Okuyama, R., Yamasaki, K., & Aiba, S. (2010). The exudate of pressure ulcers contains a substantial amount of vascular endothelial growth facotor. The Tohoku Journal of Experimental Medicine, 221(4), 315-319. http://dx.doi.org/10.1620/tjem.221.315 https://doi.org/10.1620/tjem.221.315
  20. Han, D. O., Kim, G. H., Choi, Y. B., Shim, I. S., Lee, H. J., Lee, Y. G., et al. (2005). Healing effects of astragali radix extracts on experimental open wounds in rats. Korean Journal. Oriental Physiology of Pathology, 19(1), 92-97.
  21. Kang, E. Y. (2003). Conservative management of pressure ulcers with ulmus dressing. Unpublished master's thesis, Chonnam National University, Gwangju.
  22. Kang, S. H., Oh, T. Y., Cho, H., Ahn, B. O., & Kim, W. B. (1999). Accelerated wound healing by recombinant human basic fibroblast growth factor in healing-impair-ed animal models. The Journal of Applied Pharmacology, 7(1), 7-13.
  23. Kim, H. Y., & Park, H. A. (2009). Identifying minimum datasets for pressure ulcer assessment and analysis of nursing records in home nursing. Journal of Korean Academy Community Health Nursing, 20(1), 105-111.
  24. Kim, J. M., & Park, J. S. (2010). Development of an algorithm for the prevention and management of pressure ulcers. Korean Journal of Adult Nursing, 22(4), 353-364.
  25. Kim, K. S. (2001). The effects of ulums root-bark on the pressure ulcers. Unpublished master's thesis, Chonnam National University, Gwangju.
  26. Kim, K. S., Cho, N. O., & Park, Y. S. (1997). A study on prevalence and nursing intervention of bed sore patients who received regional home care services. The Korean Journal of Fundamentals of Nursing, 4(1), 43-60.
  27. Kim, Y. O., Seo, Y. C., Lee, H. Y., Oh, S. M., Lee, S. W., & Kim, H. D. (2011). Anti-wrinkle effect of ulmus davidiana extracts. Korean Journal of Medicinal Crop Science, 19(6), 508-513. http://dx.doi.org/10.7783/KJMCS.2011.19.6.508 https://doi.org/10.7783/KJMCS.2011.19.6.508
  28. Korean Society of Pathologists. (2010). Pathology I, II. (7th ed.). Seoul: Komoonsa.
  29. Liechty, K. W., Adzick, S., & Crombleholme, T. M. (2000). Diminished interleukin 6(IL-6) production during scarless human fetal wound repair. Cytokine, 12(6), 671-676. http://dx.doi.org/10.1006/cyto.1999.0598 https://doi.org/10.1006/cyto.1999.0598
  30. Wang, H., & Keiser, J. A. (1998). Vascular endothelial growth factor upregulates the expression of matrix metalloproteinases in vascular smooth muscle cells: role of flt-1. Circulation Research, 83(8), 832-840. http://dx.doi.org/10.1161/01.RES.83. 8.832 https://doi.org/10.1161/01.RES.83.8.832

Cited by

  1. Use of Animals for Nursing Research in Korea vol.17, pp.4, 2015, https://doi.org/10.7586/jkbns.2015.17.4.324

Acknowledgement

Supported by : 경북대학교