Effects of Serum Lipid and Liver Tissue on Rats in Lectin from Viscum Album(Mistletoe)

겨우살이 Lectin이 흰쥐의 혈청지질 및 간조직에 미치는 영향

  • 장철수 (김천대학교 임상병리학과) ;
  • 류재기 (김천대학교 임상병리학과)
  • Received : 2012.04.03
  • Accepted : 2012.08.16
  • Published : 2012.09.28


This study was carried out to investigate effect of serum lipid and liver tissue in lectin from Viscum album on rats. The lectin was purified by sepharose 4B affinity chromatography and gel filtration using sephadex G-150 with plant material from Viscum album. After 72 h of $CCl_4$ injection, there was a significant increase in serum total cholesterol and triglycerige levels relative to the control group. However, treatment of both Viscum album and purified lectin were significantly decreased lipid parameters against the $CCl_4$-induced. Histological observation of the liver section of rats challenged with $CCl_4$ produced a marked increase of cytoplasmic vacuoles in number, while rats administered olive oil alone did not alter the normal hepatic architecture. Histological observation of the liver section in rats treated 72 h with either Viscum album purified lectin or $CCl_4$-induced liver lipogenesis showed decreased numbers of cytoplasmic vaculoes and necrotic cells. These results suggest that Viscum album lectin has a significantly decreased lipid in serum or histological observation of the liver section decreased in lipogenesis in rats.


  1. W. C. Boyd and E. Shapleigh, Specific precipitating activity of plant agglutinins (Lectin), Science, Vol.119, pp.419, 1954.
  2. N, Sharon and H. Lis, Lectins Cell- agglutinating and sugar- specific proteins, Science, Vol.177, pp.9, 1994.
  3. L. Lucy and I. J. Goldstein, Protein- Carbohydrate intera ction, The journal of biochemistry, Vol.243, No.8, pp.2003-2007, 1968.
  4. H. Dedray, J. M. Wieruszeski, and G. Strecker, Structral analysis of the carbohydrate chains isolated from mistletoe (Viscumalbum) lectin, Carbohydrate Research, Vol.236, pp.135-143, 1992.
  5. W. C. Boyd, The lectin. Their present status, Vox sang, Vol.8, pp.1-32, 1963.
  6. M. M. Burger and A. R. Goldberg, Identification of a tumor-specific determinant on neoplastic cell surfaces, Proc. Natl. Acad, Vol.57, pp.351-354, 1967.
  7. G. L. Nicolson, The interaction of lectins with animal cell surfaces, Int. Rev. Cytology, Vol.39, pp.89-190, 1974.
  8. A. Pih1, W. Fodstad, and S. Olsnes, Anti-cancer properties of the toxic lectins abrin and ricin, in protides of the biological fluids 27th colloquium. Peeters, H. ed. Pergamon Press, Oxford, Vol.12, pp.631-636, 1979.
  9. W. C. Greene and T. A. Waldman, Inhibition of human lymphocyte proliferation by the nonmitogenic lectin wheat germ agglutinin, J. Immunology, Vol.124, pp.2979, 1980.
  10. K. O. David and J. Bikomo, Anti diabetic properties of the african mistletoe in strepto zotocin-induced diabetic rats, J. Ethnopharma, Vol.43, pp.13-17, 1994.
  11. P. Griggs, Mistletoe, myth magic and medicine, The Biochemistry, Vol.13, pp.3-4, 1991.
  12. L. Paine and H. Harrison, Mistletoe: Its role in horticulture and human life, Hort Technology, Vol.2, pp.324-329, 1992.
  13. E. Kafaru, Mistletoe : an example of all purpose herb. The Guardian (Nigeria.), Vol.9, pp.16-17, 1993.
  14. T. J. Kim, Korean resources plants(1), Seoul University Press, pp.140-141, 1996.
  15. I. Siegle., P. Fritz, M. McClellan, S. Gutzeit and T. E. Murdter, Combined cytotoxic action of Viscum album agglutinin-1 and anticancer agents against human A 549 lung cancer cells. Anticancer Res, Vol.21, No.4A, pp.2687-2691, 2001.
  16. A. Karagoz, E. Onay, N. Arda, and A. Kuru, Antiviral potency of mistleto extracts aganist human parainfluenza virus type 2 in Vero cells. Phytother. Res. Vol.17, No.5, pp.560-562, 2003.
  17. C. S. Chang, M. J. Oh, and K. S. Roh, Purification and biochemical characterization of lectin from Viscum album, Korean J. Biotechnol. Bioeng, Vol.14, pp.578-584, 1999.
  18. C. S. Chang and K. S. Roh, Anti-diabetic effect of Viscum album lectin, Korean J. Biomed. Lab. Sci, Vol.6, pp.151-157, 2000.
  19. C. S. Chang, J. K. Ryu, and T. H. Kim, The cytotoxicity of rat hepatocytes on Viscum album lectin, Korean Soc. Clin. Lab. Sci., Vol.39, pp.190-195, 2007.
  20. B. J. Davis, Disc electrophoresis II, Ann. N. Y. Acad. Science, Vol.121, pp.404-427, 1964.
  21. C. C. Lin, J. M. Lin, C. H. Chang, M. Hattori, and T. Namba, Pharmacological studies on the crude drug Hwang-Jin- Guey' from taiwan(I), Phytotherapy research, Vol.8, pp.193-200, 1994.
  22. W. C. Schfler, Statistics for the biological sciences, Addison-Wesley Publishing Com, USA. 1980.
  23. A. K. Allen, A. Neuberger and N. Sharon, The ioslation of lectins on acid-treated agarose, Carbohyd rate Research, Vol.50, pp.121-131, 1972.
  24. R. O. Recknagel and A. K. Ghoshal, Lipoperoxidation as a vector in carbon tetrachloride hepatotoxicity, Labo. Investigation, Vol.15, pp.132-137, 1966.
  25. R. O. Recknagel, Carbon tetrachloride hepatotoxicity, Pharmacol. Reviews, Vol.19, pp.145-147, 1967.
  26. J. W. Wakasugi, K. Katami, T. ikeda, and M. Tomikawa, Action of malotilate on reduced serum cholesterol level in rats with carbon tetrachloride-induced liver damage, Japan J. Pharmacology, Vol.38, pp.391-401, 1985.