Effects of Replacing Soybean Meal with Fermented Rapeseed Meal on Performance, Serum Biochemical Variables and Intestinal Morphology of Broilers

  • Xu, F.Z. (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zeng, X.G. (Mingguang Husbandry and Veterinary Bureau) ;
  • Ding, X.L. (College of Animal Science and Technology, Anhui Agricultural University)
  • Received : 2012.05.08
  • Accepted : 2012.07.27
  • Published : 2012.12.01


This trial was performed to study the effects of replacing soybean meal (SBM) with fermented rapeseed meal (RSM) on growth performance, serum biochemistry variable and intestinal morphology of broilers. A total of 640 d-old Arbor Acres broiler chicks were randomly allocated to 4 dietary treatments, 4 pens per treatment and 40 birds per pen for a 6-wk feeding trial. In the four treatment groups, fermented RSM replaced soybean meal at 0, 5, 10, and 15%, respectively. On 21 d and 42 d, two birds from each pen were randomly selected and slaughtered. Blood samples and sections of duodenum, jejunum, and ileum were collected for measurement of serum biochemical variables and intestinal morphology, respectively. Results showed that body weight gain (BWG) and feed conversion (FC) were significantly (p<0.01) poorer for birds fed the 15% fermented RSM diet than those fed with 0, 5 and 10% fermented RSM diets during all periods. Compared with 0 and 5% fermented RSM groups, IgG content in the serum of birds in 10 and 15% fermented RSM groups was improved (p<0.01) urea nitrogen content of serum was reduced (p<0.01) during both growing and finishing periods. However, IgM, phosphorus and calcium levels increased (p<0.05) only during the growing period. Increased (p<0.05) villus height was observed in the duodenum and jejunum of broilers fed the diet with 10% fermented RSM. In addition, villus height to crypt depth ratio in the jejunum was significantly higher (p<0.01) for birds fed the diet with 10% fermented RSM than for those fed diets with 0, 5 and 15% fermented RSM. The present results suggest that RSM fermented with Lactobacillus fermentum and Bacillus subtilis is a promising alternative protein source and that it could be safely used replace up to 10% SBM in broiler diets.


  1. AOAC International. 2006. Official methods of analysis of AOAC International. 18th ed. AOAC International, Gaithersburg, MD.
  2. Bell, J. M. 1984. Nutrients and toxicants in rapeseed meal: A Review. J. Anim. Sci. 58:996-1010.
  3. Canibe, N. and B. B. Jensen. 2003. Fermented and non-fermented liquid feed to growing pigs: Effect on aspects of gastrointestinal ecology and growth performance. J. Anim. Sci. 81:2019-2031.
  4. Chah, C. C., C. W. Carlson, G. Semeniuk, I. S. Palmerand and C. W. Hesseltine. 1975. Growth-promoting effects of fermented soybeans for broilers. Poult. Sci. 54:600-609.
  5. Cheng, D. L., K. Hosimoto and Y. Uda. 2004. In vitro digestion of sinigrin and glucotropeolin by single strain of Bifidobacterium and identification of digestive products. Food Chem. Toxicol. 42:351-357.
  6. Chiang, G., W. Q. Lu, X. S. Piao, J. K. Hu, L. M. Gong and P. A. Thacker. 2010. Effects of feeding solid-state fermented rapeseed meal on performance, nutrient digestibility, intestinal ecology and intestinal morphology of broiler chickens. Asian-Aust. J. Anim. Sci. 23:263-271.
  7. Chibowska, M., S. Smulikowska and B. Pastuszewska. 2001. Metabolisable energy value of rapeseed meal and its fractions for broiler chickens as affected by oil and fibre content. J. Anim. Feed Sci. 9:371-378.
  8. Choi, M. M. F., S. M. Shuang, H. Y. Lai, S. C. Cheng, R. C. W. Cheng, B. K. B. Cheung and A.W.M. Lee. 2004. Gas chromatography-mass spectrometric determination of total isothiocyanates in Chinese medicinal herbs. Anal. Chem. Acta. 516:155-163.
  9. Davis, G. S., K. G. Anderson and A. S. Carrol. 2000. The effects of long-term caging and molt of Single Comb White Leghorn hens of heterophil to lymphocyte ratios, corticosterone and thyroid hormones. Poult. Sci. 79:514-518.
  10. Ebune, A., S. Al-Asheh and Z. Duvnjak. 1995. Production of phytase during solid state fermentation using Aspergillus ficuum NRRL 3135 in canola meal. Bioresour. Technol. 53:7-12.
  11. Elangovan, A. V. S., V. S. Verma, V. R. B. Sastry and S. D. Singh. 2001. Effect of feeding high glucosinolate rapeseed meal to laying Japanese Quail. Asian-Aust. J. Anim. Sci. 14:1304-1307.
  12. El-Batal, A. I. and H. Abdel Karem. 2001. Phytase production and phytic acid reduction in rapeseed meal by Aspergillus niger during solid state fermentation. Food Res. Int. 34:715-720.
  13. El-Batal, A. I., M. A.Abo-State and A. Shihab. 2000. Phenylalanine ammonia lyase production by gamma irradiated and analog-resistant mutants of Rhodotorula glutinis. Acta Microbiol. Pol. 49:51-61.
  14. Fadel, M. and A. I. El-Batal. 2000. Studies on activation of amylolytic enzymes production by gamma irradiated Aspergillus niger using some surfactants and natural oils under solid state fermentation. Pakistan J. Biol. Sci. 3:1762-1768.
  15. Fang, Z. F., J. Peng, Z. L. Liu and Y. G. Liu. 2007. Responses of non-starch polysaccharide-degrading enzymes on digestibility and performance of growing pigs fed a diet based on corn, soya bean meal and Chinese double-low rapeseed meal. J. Anim. Physiol. Anim. Nutr. (Beri). 91:361-368.
  16. Feng, J., X. Liu, Z. R. Xu, Y. Y. Liu and Y. P. Lu. 2007. Effects of Aspergillus oryzae 3.042 fermented soybean meal on growth performance and serum biochemical variable in broilers. Anim. Feed Sci.Technol. 134:235-242.
  17. Guyton, A. C. 1986. The thyroid metabolic hormones. Page 897 in Medical Physiology (Ed. D. Dreibelbis). Saunders, Philadephia, PA.
  18. Ishikawa, T. and F. Nanjo. 2009. Dietary cycloinulooligosaccharides enhance intestinal immunoglobulin A production in mice. Biosci. Biotechnol. Biochem. 73:677-682.
  19. Karunajeewa, H., E. G. Ijagbuji and R. L. Reece. 1990. Effect of dietary levels of rapeseed meal and polyethylene glycol on the performance of male broiler chickens. Br. Poult. Sci. 31:545-556.
  20. Kiers, J. L., A. E. A. Van Laeken, F. M. Rombouts and M. J. R. Nout. 2000. In vitro digestibility of bacillus fermented soya bean. Int. J. Food Microbiol. 60:163-169.
  21. Kumakura, M. 1993. Dose-dependency of radiation on enzyme production in Trichoderma reesei. Radiat. Environ. Biophys. 32:41-46.
  22. Lardy, G. P. and M. S. Kerley. 1994. Effect of increasing the dietary level of rapeseed meal on intake by growing beef steers. J. Anim. Sci. 72:1936-1942.
  23. Lee, P. A. and R. Hill. 1983. Voluntary food intake of growing pigs given diets containing rapeseed meal, from different types and varieties of rape, as the only protein supplement. Br. J. Nutr. 50:661-671.
  24. Marczak, E. D., H. Usui, H. Fujita, Y. J. Yang, M. Yokoo, A. W. Lipkowski and M. Yoshikawa. 2003. New antihypertensive peptides isolated from rapeseed. Peptides 24:791-798.
  25. McNeill, L., K. Bernard and M. G. MacLeod. 2004. Food intake, growth rate, food conversion and food choice in broilers fed on diets high in rapeseed meal and pea meal with observations of the resulting poultry meat. Br. Poult. Sci. 45:519-523.
  26. Miller, B. G., T. J. Newby, C. R. Stokes and F. J. Boame. 1984. Influence of diet on post-weaning malabsorption and diarrhoea in the pig. Res. Vet. Sci. 36:187-193.
  27. Montaqne, J. P. and C. I. Faure. 1977. How to interpret an x-ray by upper approach of the small intestine in children. Arch. Fr. Pediatr. 34:90-104.
  28. Palander, S., M. Nasi and I. Ala-fossi. 2004. Rapeseed and soybean products as protein sources for growing turkeys of different ages. Br. Poult. Sci. 45:664-671.
  29. Pluske, J. R., M. J. Thompson, C. S. Atwood, P. H. Bird, L. H. Williams and P. E. Hartmenn. 1996. Maintenance of villus height and crypt depth, and enhancement of disaccharide digestion and monosaccharide absorption, in piglets fed on cows' whole milk after weaning. Br. J. Nutr. 76:409-422.
  30. Rajoka, M. I., A. Bashir, S. R. S. Hussain and K. A. Malik. 1998. $\gamma$-Ray induced mutagenesis of Cellulomonas biazota for improved production of cellulases. Folia Microbiol. 43:15-22.
  31. SAS Institute. 1996. SAS user's guide: Statistics. Version 7.0. SAS Institute, Cary, NC, USA.
  32. Spiegel, C. and J. W. Blum. 1993. Lower food intake is a primary cause of reduced growth rate in growing pigs fed rapeseed presscake meal. J. Nutr. 123:1562-1566.
  33. Tripathi, M. K. and A. S. Mishra. 2006. Glucosinolates in animal nutrition: A review. Anim. Feed Sci. Technol. 132:1-27.
  34. Verbiscar, A. J., T. F. Banigan, C. W Weber, B. L. Reid, R. S. Swingle, J. E. Trei and E. A. Nelson. 1981. Deotoxification of jojoba meal by lactobacilli. J. Agric. Food. Chem. 29:296-302.
  35. Vig, A. P. and A. Walia. 2001. Beneficial effects of Rhizopus oligosorus fermentation on reduction of glucosinolates, fibre and phytic acid in rapeseed (Brassica napus) meal. Bioresour. Technol. 78:309-312.
  36. Wang, J. F., Y. H. Zhu and D. F. Li. 2004. In vitro fermentation of various fiber and starch sources by pig fecal inocula. J. Anim. Sci. 82:2615-2622.
  37. Wang, T., Y. M. Fu, J. L. Lv, H. S. Jiang, Y. P. Li, C. Y. Chen and C. M. Zuo. 2003. Effects of mini-peptides on the growth performance and the development of small intestines in weaning piglets. Anim. Husbandry & Vet. Medicine. 6:4-8.
  38. Xu, F. Z., L. M. Li, J. P. Xu, K. Qian, Z. D. Zhang and Z. Y. Liang. 2011. Effects of fermented rapeseed meal on growth performance and serum parameters in ducks. Asian-Aust. J. Anim. Sci. 24:678-684.
  39. Xue, Z. H., W. C. Yu, M. C. Wu and J. H. Wang. 2009. In vivo antitumor and antioxidative effects of a rapeseed meal protein hydrolysate on an S180 tumor-bearing murine model. Biosci. Biotechnol. Biochem. 73:2412-2415.

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