In vitro propagation and multiple shoot induction of Rhodiola rosea L. by axillary bud culture

홍경천(Rhodiola rosea L.)의 액아배양을 통한 다신초 유도 및 기내 대량증식

  • Received : 2012.06.15
  • Accepted : 2012.07.20
  • Published : 2012.06.30


An efficient in vitro propagation was established by using axillary bud explants of roseroot (Rhodiola rosea L.), which has been known as a medicinal plant in East Asia. Among various media tested, MS medium supplemented with 1.0 mg/L BA and 1.0 mg/L $GA_3$ was found to be the best for multiple shoot formation (15 axillary shoots per axillary bud). In addition 1/2MS medium containing 50 g/L sucrose was best for shoot elongation (7.8 cm) and increasing total chlorophyll contents (8.64 mg/g) best. Maximum number of roots (17.7 roots per explant) was observed on the medium without plant growth regulators. Propagated plants were successfully acclimatized to ex vitro conditions, with a survival frequency of 97% after 12 weeks. Most rooted shoots grew well and produced viable seeds when grown in vitro culture conditions. Therefore, R. rosea can be effectively propagated in vitro by the system we developed in this study.


axillary bud culture;in vitro rooting;in vitro propagation;Rhodiola rosea


  1. Afanasʼev SA, Krylatov AV, Lasukosa TV, Lishmanov YB (1996) Participation of inducible stress proteins in the cardioprotective effects of Rhodiolae rosea. Biochem (Moscow). 61:1258-1263
  2. Ahn CH, Bae KH, Yi JS, Choi YE (2008) Induction and growth of adventitious roots and bioreactor culture in Codonopsis lanceolata. Kor J Plant Biotech 35:155-161
  3. Bae KH, Lim S, Yoon ES, Shin CG, Kim YY, Kim YS (2005) Effect of cytokinin and putrescine on plant regeneration from leaf explant of Rhodiola sachalinensis A. Bor. Kor J Plant Biotech 32:195-199
  4. Bae KH, Yoon ES, Choi YE (2005) In vitro culture of adventitious root from Rhodiola sachalinensis. Kor J Plant Res 4:281-286
  5. Cho EY, Roh KS (2008) Influence of ammonium against Cadmium effect on in vitro growth, chlorophyll and Rubisco/Rubisco activase in Tobacco leaves. J Life Sci 5:660-667
  6. Choi DY, Ahn SY, Lee SG, Han JS, Kim EC, Lee HB, Shin JH, Kim EK, Row KH (2004) Separation and performance test of whitening agent in Rhodiola sachalinensis. Kor J Plant Biotech Bioeng 3:169-173
  7. Choi HJ, Kim SJ, Hwang B, Ahn JC (2005) Optimization of treatment concentration and screening of exogenous plant growth regulators for improvement of salidroside yield in Rhodiola sachalinensis A Bor cell suspension culture. Kor J Plant Biotech 2:105-109
  8. Choi KM, Hwang SJ, Ahn JC, Lee HY, Kim JH, Hwang B (2005) In vitro propagation from axillary bud explant of Fatsia japonica Deene. et Planch. Kor J Med Crop Sci 13:300-303
  9. Choi YE, Kim JW, Soh WY (1997a) Somatic embryogenesis and plant regeneration from suspension cultures of Acanthopanax koreanum. Plant Cell Rep 17:84-88
  10. Choi YE, Kim HS, Yang DC, Choi KT (1997b). Distribution and changes of reserve materials in cotyledon cells of Panax ginseng related to direct somatic embryogenesis and germination. Plant Cell Rep 16:738-744.
  11. Chung TH (1974) Korean flora (Herb part). Seoul, Academybook. pp 283
  12. Debnath SC (2009) Zeatin and TDZ-induced shoot proliferation and use of bioreactor in clonal propagation of medicinal herb, roseroot (Rhodiola rosea L). J Plant Biochem Biot 18(2): 245-248
  13. Hong EY, Kim IH, Yun JS, Yun T, Lee CH (2003) Growth characteristics and search for eligible cultivation area of Rhodiola sachalinensis A. Bor. Kor J Plant Res 16:212-217
  14. Kim CH, Kwon MC, Han JG, Ha JH, Jeong HS, Choi GP, Park UY, Nam JH, Hwang B, Lee HY (2008) Immune activities of Rhodiola sachalinensis A. Bor. extracts isolated with various extraction process. Kor J Med Crop Sci 16:383-389
  15. Kim JA, You XL, Ahn CH, Lee JS, Choi YE (2007) Plant Regeneration via direct adventitious roots from free root segments of Ulmus davidiana Planch. J Korean Forest Sci 96(1): 83-88
  16. Kim JY, Seong NS, Lee YJ (2006a) A study on the effects of Rhodiola rosea root on the cancers. Kor J of Herb 21:79-87
  17. Kim ML, Nam DW, Ahn JC, Hwang B (2006b) Micropropagation of Hypericum erectum by axillary bud culture. Kor J Med Crop Sci 14:23-26
  18. Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth Enzymol 148:350-382
  19. Linch PT, Kim YH, Hong SP, Jian JJ, Kang JS (2000) Quantitative determanation of salidroside and tyrosol from the chromatography. Arch Pharm Res 23:349-352
  20. Lishmanov YB, Maineskulova LA, Uskina EV, Maslov LN (1999) Opiatergic mechanisms of cardioprotective and antiarrhythmic effects of adaptation. Bull Exp Bio and Medi 127:151-154
  21. Ming HQ, Xia GC, Zhang RD (1988) Advanced research on Rhodiola. Chin Trad Herbal Drug 19:229-234
  22. Moon HK, Noh EW, Ha YM, Shim KK (2002) Micropropagation of juvenile and mature tree of Corylopsis coreana by axillary bud culture. Kor J Plant Tiss Cult 29:117-121
  23. Moon HK, Suk GY, Kwon YJ, Son SH (1999) Micropropagation of a rare species, Abeliophyllum distichum Nakai. via axillary bud culture. Kor J Plant Tiss Cult 26:133-136
  24. Moon HK, Kim YW (2008) In vitro propagation of a rare species, Echinosophora koreensis Nakai. by axillary bud culture. Kor J Plant Tiss Cult 35:229-234
  25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-479
  26. Park HU, Yoon JH, Kim JY, Jeong CH, Park CK, Song WS, Seo KI (2005) Biological activity of the fractions extracted from Rhodiola dumulosa. Kor Soc Food Pres 12:596-500
  27. Petkov VD, Yonkov D, Mosharoff A, Kambourova T, Alova L, Petkov V, Todorov I (1986) Effects of alcohol aqueous extract from Rhodiola rose L. root on learning and memory. Act Physiol Pharmacol Bulg 12:3-16

Cited by

  1. Plant regeneration and soil acclimatization through photoautotrophic culture from leaf explant of a rare species in Sedum tosaense Makino vol.40, pp.2, 2013,
  2. In vitro propagation of endangered species, Hylotelephium ussuriense (Kom.) H. Ohba vol.41, pp.1, 2014,
  3. Effect of kinds of medium and plant growth regulators for plantlets regeneration by bud culture in Disporum sessile vol.44, pp.1, 2017,