DOI QR코드

DOI QR Code

Bioassay in BALB/c mice exposed to low dose rate radiation

저선량율 방사선 조사한 BALB/c 마우스에서의 영향평가

  • Kim, Sung-Dae (Dongnam Institute of Radiological & Medical Sciences) ;
  • Gong, Eun-Ji (Dongnam Institute of Radiological & Medical Sciences) ;
  • Bae, Min-Ji (Dongnam Institute of Radiological & Medical Sciences) ;
  • Yang, Kwang-Mo (Dongnam Institute of Radiological & Medical Sciences) ;
  • Kim, Joong-Sun (Dongnam Institute of Radiological & Medical Sciences)
  • Received : 2012.06.20
  • Accepted : 2012.07.14
  • Published : 2012.09.30

Abstract

The present study was performed to investigate the toxicity of low-dose-rate irradiation in BALB/c mice. Twenty mice of each sex were randomly assigned to four groups of five mice each and were exposed to 0 (sham), 0.02, 0.2, or 2 Gy, equivalents to low-dose-rate irradiation to 3.49 $mGy{\cdot}h^{-1}$. Urine, blood, and blood biochemistry were analyzed, and organ weight was measured. The low-dose-rate irradiation did not induce any toxicologically significant changes in mortality, clinical signs, body weight, food and water consumption, urinalysis, and serum biochemistry. However, the weights of reproductive organs including the testis, ovary, and uterus decreased in a dose-dependent manner. Irradiation at 2 Gy significantly decreased the testis, ovary, and uterus weights, but did not change the weights of other organs. There were no adverse effects on hematology in any irradiated group and only the number of neutrophils increased dose dependently. The low-dose-rate irradiation exposure did not cause adverse effects in mice at dose levels of 2 Gy or less, but the reproductive systems of male and female mice showed toxic effects.

Acknowledgement

Supported by : 동남권원자력의학원

References

  1. Hall E. Radiobiology for the radiologist, 2nd. Harper & Row, Publishers, Inc. 1978:63-78.
  2. De Saint-Georges L. Low-dose ionizing radiation exposure: understanding the risk for cellular transformation. J. Biol. Regul. Homeost. Agents 2004;18(2):96-100.
  3. Nakamura H, Yasui Y, Saito N, Tachibana A, Komatsu K, Ishizaki K. DNA repair defect in AT cells and their hypersensitivity to low-dose-rate radiation. Radiat. Res. 2006;165(3):277-282. https://doi.org/10.1667/RR3519.1
  4. Ina Y, Sakai K. Activation of immunological network by chronic low-dose-rate irradiation in wild-type mouse strains: analysis of immune cell populations and surface molecules. Int. J. Radiat. Biol. 2005;81(10):721-729. https://doi.org/10.1080/09553000500519808
  5. Ina Y, Tanooka H, Yamada T, Sakai K. Suppression of thymic lymphoma induction by life-long low-dose-rate irradiation accompanied by immune activation in C57BL/6 mice. Radiat. Res. 2005;163(2):153-158. https://doi.org/10.1667/RR3289
  6. Moreno SG, Dutrillaux B, Coffigny H. High sensitivity of rat foetal germ cells to low dose-rate irradiation. Int. J. Radiat. Biol. 2001;77(4):529-538. https://doi.org/10.1080/09553000010030211
  7. UNSCER. Volume I and II. United Nations sales publication E.00.IX.3 and E.00.IX.4 United Nations, New York. 2000.
  8. OECD (Organisation of Economic Co-operation and Development). Guidelines for the Testing of Chemicals. No. 401:Acute Oral Toxicity, Paris; 1987.
  9. Kang YM, Shin SC, Jin YW, Kim HS. Changes in body and organ weights, hematological parameters, and frequency of micronuclei in the peripheral blood erythrocytes of ICR mice exposure to low dose rate Υ-radiation. Journal of Radiation Protection. 2009;34(3):102-106.
  10. Tsiperson VP, Soloviev MY. The impact of chronic radioactive stress on the immuno-physiological condition of small mammals. Sci. Total Environ. 1997;203(2):105-113. https://doi.org/10.1016/S0048-9697(97)00138-1
  11. Jang JS, Kim JS, Kim JC, Kim SH. Evaluation of Radiation-induced Apoptosis in Seminiferous Tubule of ICR Mouse after Gamma Irradiation. Journal of Life Science 2009;19(6): 799-803. https://doi.org/10.5352/JLS.2009.19.6.799
  12. Liu G, Gong P, Zhao H, Wang Z, Gong S, Cai L. Effect of low-level radiation on the death of male germ cells. Radiat. Res. 2006;165(4):379-389. https://doi.org/10.1667/RR3528.1
  13. Lee YK, Chang HH, Kim WR, Kim JK, Yoon YD. Effects of gamma-radiation on ovarian follicles. Arh. Hig. Rada. Toksikol. 1998;49(2):147- 153.
  14. Dobson RL, Felton JS. Female germ cell loss from radiation and chemical exposures. Am. J. Ind. Med. 1983;4:175-190. https://doi.org/10.1002/ajim.4700040116
  15. Chapman RM. Effect of cytotoxic therapy on sexuality and gonadal function. Semin. Oncol.1982; 9(1):84-94.
  16. Wolford ST, Schroer RA, Gohs FX, Gallo PP, Brodeck M, Falk HB, Ruhren R. Reference range data base for serum chemistry and hematology values in laboratory animals. J. Toxicol. Environ. Health 1986;18(2):161-188. https://doi.org/10.1080/15287398609530859
  17. Lee DK, Kang HJ. Effects on Heamopoietic Cells and Chromosomal DNA of Leukocytes in Mice Irradiated with Low Dose of X-ray I. Changes of Leukocyte, Lymphocyte, Thrombocyte and Erythrocyte Number. Kor. J. Vet. Publ. Hlth. 2002; 26:143-152.
  18. Li W, Wang G, Cui J, Xue L, Cai L. Low-dose radiation (LDR) induces hematopoietic hormesis: LDR-induced mobilization of hematopoietic progenitor cells into peripheral blood circulation. Exp. Hematol. 2004;32(11):1088-1096. https://doi.org/10.1016/j.exphem.2004.07.015
  19. Kucerova M, Anderson AJ, Buckton KE, Evans HJ. X-ray-induced chromosome aberrations in human peripheral blood leucocytes: the response to low levels of exposure in vitro. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 1972;21(4):389-396. https://doi.org/10.1080/09553007214550451

Cited by

  1. Continuous Exposure to Low-Dose-Rate Gamma Irradiation Reduces Airway Inflammation in Ovalbumin-Induced Asthma vol.10, pp.11, 2015, https://doi.org/10.1371/journal.pone.0143403
  2. Low-dose-rate radiation exposure leads to testicular damage with decreases in DNMT1 and HDAC1 in the murine testis vol.55, pp.1, 2014, https://doi.org/10.1093/jrr/rrt090
  3. DOSE AND DOSE RATE EFFECTS OF IRRADIATION ON BLOOD COUNT AND CYTOKINE LEVEL IN BALB/c MICE vol.38, pp.4, 2013, https://doi.org/10.14407/jrp.2013.38.4.179