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Effect of Diethyldithiocarbamate on Radiation-induced Learning and Memory Impairment in Mouse

방사선 유도 학습기억 장애에 대한 diethyldithiocarbamate의 효과

  • Jang, Jong-Sik (Faculty of Animal Science & Biotechnology, Kyungpook National University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University, Animal Medical Center, Chonnam National University) ;
  • Moon, Chang-Jong (College of Veterinary Medicine, Chonnam National University, Animal Medical Center, Chonnam National University) ;
  • Jung, U-Hee (Advanced Radiation Technology Institute) ;
  • Jo, Sung-Kee (Advanced Radiation Technology Institute) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University, Animal Medical Center, Chonnam National University)
  • Received : 2012.08.07
  • Accepted : 2012.09.06
  • Published : 2012.09.30

Abstract

Evidence suggests that even low-dose irradiation can lead to progressive cognitive decline and memory deficits, which implicates, in part, hippocampal dysfunction in both humans and experimental animals. This study examined whether diethyldithiocarbamate (DDC) could attenuate memory impairment, using passive avoidance and object recognition test, and suppression of hippocampal neurogenesis, using the TUNEL assay and immunohistochemical detection with markers of neurogenesis (Kiel 67 (Ki-67) and doublecortin (DCX)) in adult mice treated with gamma radiation (0.5 or 2 Gy). DDC was administered intraperitonially at a dosage of 1,000 $mg{\cdot}kg^{-1}$ of body weight at 30 min. before irradiation. In passive avoidance and object recognition memory test, the mice, trained for 1 day after acute irradiation (2 Gy) showed significant memory deficits compared with the sham controls. The number of TUNEL-positive apoptotic nuclei in the dentate gyrus (DG) was increased 12 h after irradiation. In addition, the number of Ki-67- and DCX-positive cells were significantly decreased. DDC treatment prior to irradiation attenuated the memory defect, and blocked the apoptotic death. DDC may attenuate memory defect in a relatively low-dose exposure of radiation in adult mice, possibly by inhibiting a detrimental effect of irradiation on hippocampal neurogenesis.

Acknowledgement

Supported by : 한국과학재단

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