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Quinine Assay with Home-Built UV-LED Fluorometer: Quantitative Analysis, Photo-Bleaching, Fluorescence Quenching, and Urine Analysis

  • Cheon, Tae-Min ;
  • Cheong, Byeong-Seo ;
  • Cho, Han-Gook ;
  • Kim, Jin-Hee ;
  • Kim, Kyoug-Soo
  • Received : 2012.08.25
  • Accepted : 2012.09.02
  • Published : 2012.10.20

Abstract

Quinine quantitative analysis, photo-bleaching, fluorescence quenching, and urine analysis have been performed by means of a UV-LED fluorometer, which can be easily built and used in a high-school laboratory. The quinine detection range is estimated to be 0.05-80 ppm, enough for many classroom luminescence experiments. The quinine content in commercial tonic water is determined from the calibration curve, and UV photo-bleaching of this anti-malarial drug is demonstrated with clear wavelength dependence. Halide quenching of quinine fluorescence is also observed and the increase in quenching efficiency in the order of $Cl^-$, $Br^-$, and $I^-$ is evident. Urine analyses for the student volunteers have been carried out and the results clearly reveal excretion of the ingested quinine. The student participants are exuberant throughout the course of this study and sense the practices resourceful.

Keywords

Quinine;Fluorescence;UV-LED;Fluorescence quenching;Urine analysis

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Acknowledgement

Supported by : University of Incheon