Response of Phytotoxicity on Rice Varieties to HPPD-inhibiting Herbicides in Paddy Rice Fields

HPPD 저해 제초제에 대한 벼 품종별 약해 반응

  • Kwon, Oh-Do (Jeonnam Agricultural Research and Extension Service) ;
  • Shin, Seo-Ho (Jeonnam Agricultural Research and Extension Service) ;
  • An, Kyu-Nam (Jeonnam Agricultural Research and Extension Service) ;
  • Lee, Yeen (Jeonnam Agricultural Research and Extension Service) ;
  • Min, Hyun-Kyeng (Jeonnam Agricultural Research and Extension Service) ;
  • Park, Heung-Gyu (Jeonnam Agricultural Research and Extension Service) ;
  • Shin, Hae-Ryoung (Jeonnam Agricultural Research and Extension Service) ;
  • Jung, Ha-Il (Department of Crop and Soil Sciences, Cornell University) ;
  • Kuk, Yong-In (Dept. of Development in Resource Plants, College of Life Science and Natural Resources, Sunchon National University)
  • 권오도 (전남농업기술원 쌀연구소) ;
  • 신서호 (전남농업기술원 쌀연구소) ;
  • 안규남 (전남농업기술원 쌀연구소) ;
  • 이인 (전남농업기술원 쌀연구소) ;
  • 민현경 (전남농업기술원 쌀연구소) ;
  • 박흥규 (전남농업기술원 쌀연구소) ;
  • 신해룡 (전남농업기술원 쌀연구소) ;
  • 정하일 (코넬대학교 작물 및 토양학과) ;
  • 국용인 (순천대학교 생명산업과학대학 한약자원개발학과)
  • Received : 2012.08.22
  • Accepted : 2012.09.19
  • Published : 2012.09.30


The objectives of this study were to investigate the levels of phytotoxicity of rice varieties to HPPD (4-hydroxy phenylpyruvate dioxygenase)-inhibiting herbicides known for their efficiency to control the sulfonylureas-resistant weed species:mestrione, benzobicyclone, and tefuryltrione. The twenty-six rice varieties (8-Japonica ${\times}$ Indica-type varieties and 18-Japonica-type varieties) were grown for 25 days on seedling trays and then transplanted to paddy rice fields followed by herbicide treatment i.e. standard and double doses of there respective herbicides at 5, 10, and 15 days after transplanting. Although mestrione, benzobicyclone and tefuryltrione are all HPPD-inhibiting herbicides, the phytotoxicity symptoms of the different rice varieties based on the timing of application and doses of the herbicides were significantly different. The Japonica ${\times}$ Indica-type varieties showed much more phytotoxicity symptoms than Japonica-type varieties in all applied herbicides. Increasing herbicidal doses of mesotrione, and an earlier application of and increasing herbicidal doses of benzobicyclon caused severe phytotoxicity symptoms. On the other hand, phytotoxicity due to tefuryltrione did not exhibit significant differences between rice varieties in either the timing of application or dose of the herbicide. Regardless of timing of application and dose of the herbicides, Hangangchalbyeo-1, Hyangmibyeo-1 and high-yield rice varieties such as Namcheonbyeo, Dasanbyeo, Areumbyeo, and Hanareumbyeo, which belong to the Japonica ${\times}$ Indica-type varieties, showed 5 to 8 levels of phytotoxicity symptoms including albinism, browning, detached leaf, and necrosis to mesotrione and benzobicyclon whereas only 1 to 3 levels of phytotoxicity symptoms (chlorosis, albinism, and browning) were seen with to tefuryltrione application. The Japonica-type varieties exhibited only slight phytotoxicity symptoms (1~2 levels) in conformity with the timing of application and doses of the herbicides. However, there were significant differences among the Japonica-type rice varieties, depending on the type of herbicide. Thirteen-Japonica type rice varieties were sensitive to benzobicyclone while 4-Japonica-type and 7-Japonica-type varieties showed phytotoxicity symptoms such as chlorosis and albinism with mestrione and tefuryltrione application, respectively. Therefore, we suggest that the combined-type herbicides including mestrione, benzobicyclone and tefuryltrione should be rejected in paddy fields where rice is grown for either human consumption (functional or processed rice) or livestock feed because of severe phytotoxicity symptoms on the various rice varieties seen regardless of the timing of application and doses of the herbicides.


  1. Abit, M. J. M., and K. Al-Khatib. 2009. Absorption, translocation and metabolism of mesotrione in grain sorghum. Weed Sci. 57:563-566.
  2. Barta, I. C., and P. Boger. 1996. Purification and characterization of 4-hydroxyphenylpyruvate dioxygenase from maize. Pestic. Sci. 48:109-116.<109::AID-PS447>3.0.CO;2-7
  3. Han S. S., K. Y. Yoo, M. S. Park, and D. W. Kang. 2009. Reactivity of herbicide benzobicyclon in rice and weed rice. Korean J. Weed Sci. 29 (Supp. 2):105.
  4. Im, I. B., S. Kim, S. H. Ahn, X. H. An, and S. H. Cho. 2008. Control characteristics of weed (red) rice (Oryza sativa L.) by benzobicyclon application. Korean J. Weed Sci. 28(2):181-188.
  5. Kim, S. Y., J. Y. Lee, U. S. Yeo, S. H. Oh, S. T. Park, J. H. Lee, K. H. Jeong, J. H. Cho, Y. C. Song, and H. W. Kang. 2010. Differential tolerance of rice cultivars to mesotrione-contained herbicides. Korean J. Weed Sci. 30(3):300-307.
  6. Komatsubara, K., K. Sekino, Y. Yamada, H. Koyanagi, and S. Nakahara. 2009. Discovery and development of a new herbicide, benzobicyclon. J. Pestic. Sci. 34(2):113-114.
  7. Korea Crop Protection Association (KCPA). 2011. Guide book of using the agrochemicals. Sam Jeong Press Co., Seoul. 1309p.
  8. Lee, D. L., M. P. Prisbylla, T. H. Cromatie, D. P. Dagarin, S. W. Howard, W. M. Provan, M. K. Ellis, T. Fraser, and L. C. Mutter. 1997. The discovery of P-hydroxyphenylpyruvate dioxygenase. Weed Sci. 45:601-609.
  9. Lee, I. Y., C. S. Kim, J. Lee, B. C. Moon, and Y. G. Jeong. 2011. Biological characteristics of bromobutide+ imazosulfuron+mefenacet GR using paddy fields. Korean J. Weed Sci. 31(4):401-404.
  10. Pallett, K. E., J. P. Little, P. Veerasekaran, and F. Viviani. 1997. Inhibition of 4-hydroxyphenylpyruvate dioxygenase:the mode of action of the herbicide RPA 201772 (isoxaflutole). Pestic. Sci. 50:83-84.<83::AID-PS554>3.0.CO;2-S
  11. Park, T. S, I. Y. Lee, K. Y. Seong, H. S. Cho, H. K. Park, J. K. Ko, and U. G. Kang. 2011. Status and prospect of herbicide resistant weeds in rice field of Korea. Korean J. Weed Sci. 31(2):119-133.
  12. Ryang, H. S., and I. K. Kim. 1986. Studies on factors affecting the injury caused by simetryne to rice plants of Tongil variety. Korean J. Weed Sci. 6(1): 48-58.
  13. Ryang, H. S., S. S. Han, and J. S. Kim. 1981. Weeding effect and phytotoxicity variable in herbicide treatment in mechanically transplanted paddy field 1. Effect of application time on weeding effect and phytotoxicity. Korean J. Weed Sci. 1(1):69-77.
  14. Sekino, K. 2002. Discovery study of new herbicides from the inhibition of photosynthetic pigments biosynthesis (development of a new plastoquinone biosynthetic inhibitor, benzobicyclon as a herbicide). Japanese J. Weed Sci. 27:388-391.
  15. Sekino, K., H. Koyanagi, E. Ikuta, and Y. Yamada. 2008. Herbicidal activity of a new paddy bleaching herbicide, benzobicyclon. J. Pestic. Sci. 33(4), 364-370.
  16. Song, J. E., M. S. Park, J. H. Jeong, E. H. Park, and C. K. Jeong. 2011. Herbicidal efficacy affected by different formulation of benzobicyclon-mixtures herbicides in paddy rice field. Korean J. Weed Sci. 31(4):384-393.
  17. 양원하, 한희석, 안종웅, 곽창길, 이충근, 손지영, 김준환, 이미현, 이은형, 안치중. 2010. 제초제 벤조비 사이클론(Benzobicyclon)에 대한 초다수성벼와 특수미의 약해정도. 한국작물학회지 55(S1):40.
  18. 한국작물보호협회. 2012. 작물보호제 지침서. 1351p.

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