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Isolation, Identification and Characterization of Paraquat Activity-Inhibiting Substances in Squash (Cucurbita moschata Duchesne ex Poiret) Leaves

호박잎에서 Paraquat 활성 억제 물질의 분리, 동정 및 특성 구명

  • Hyun, Kyu-Hwan (Dept. of Development in Resource Plants, College of Life Science and Natural Resources, Sunchon National University) ;
  • Yun, Young-Beom (Dept. of Development in Resource Plants, College of Life Science and Natural Resources, Sunchon National University) ;
  • Jang, Se-Ji (Dept. of Development in Resource Plants, College of Life Science and Natural Resources, Sunchon National University) ;
  • Shin, Dong-Young (Dept. of Development in Resource Plants, College of Life Science and Natural Resources, Sunchon National University) ;
  • Kwon, Oh-Do (Jeonnam Agricultural Research and Extension Service) ;
  • Choi, Hyun-Sug (Department of Agro-Food Safety, National Academy of Agricultural Science, RDA) ;
  • Jung, Ha-Il (Department of Crop and Soil Sciences, Cornell University) ;
  • Kuk, Yong-In (Dept. of Development in Resource Plants, College of Life Science and Natural Resources, Sunchon National University)
  • 현규환 (순천대학교 생명산업과학대학 한약자원개발학과) ;
  • 윤영범 (순천대학교 생명산업과학대학 한약자원개발학과) ;
  • 장세지 (순천대학교 생명산업과학대학 한약자원개발학과) ;
  • 신동영 (순천대학교 생명산업과학대학 한약자원개발학과) ;
  • 권오도 (전남농업기술원 쌀연구소) ;
  • 최현석 (국립농업과학원 유기농업과) ;
  • 정하일 (미국 코넬대학교 작물토양학과) ;
  • 국용인 (순천대학교 생명산업과학대학 한약자원개발학과)
  • Received : 2012.09.03
  • Accepted : 2012.09.19
  • Published : 2012.09.30

Abstract

The fourth leaves (younger leaves) amongst extended 4-upper leaves in 18 squash cultivar were the highest tolerance to the paraquat application, followed by third, the second, and the first leaves (older leaves). The forth leaves in Joongangaehobak showed more than three times higher tolerance to the paraquat application than did the first leaves. When the combining of water extract from the fourth leaves with paraquat were applied to the leaves and stems of maize, the paraquat phytotoxicity in maize was reduced compared to the paraquat application alone. Therefore, this study continued to investigate if the phytotoxicity inhibitor exist in the fourth leaves. The water extract in the fourth leaves were isolated by silica gel column chromatography, Sephadex LH-20 column chromatography, TLC, and HPLC, and the substance in the extract was speculated as a malic acid by identifying through NMR. The mixture malic acid and paraquat were applied to the maize to verify the application effect of malic acid on paraquat toxicity. The 100 ${\mu}M$ of paraquat application alone showed 62% of paraquat toxicity to the corn leaves, while the combined application of 100 ${\mu}M$ paraquat with malic acid at 0.1, 0.3, 0.5, and 1.0% did not show the symptom.

Acknowledgement

Supported by : 연구재단

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