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Pseudomonas aeruginosa P-5 균주로부터 3-Hydroxyvalerate와 Medium-chain-length 3-hydroxyalkanoates로 구성된 공중합체의 생합성

Biosynthesis of Copolyesters Consisting of 3-Hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates by the Pseudomonas aeruginosa P-5 Strain

  • 우상희 (충남대학교 미생물.분자생명과학과) ;
  • 김재희 (충남대학교 미생물.분자생명과학과) ;
  • 예우양 (충남대학교 미생물.분자생명과학과) ;
  • 이영하 (충남대학교 미생물.분자생명과학과)
  • Woo, Sang-Hee (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Kim, Jae-Hee (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Ni, Yu-Yang (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Rhee, Young-Ha (Department of Microbiology and Molecular Biology, Chungnam National University)
  • 투고 : 2012.07.31
  • 심사 : 2012.09.13
  • 발행 : 2012.09.30

초록

활성슬러지로부터 특이한 조성의 polyhydroxyalkanoates (PHAs)를 생합성하는 Pseudomonas aeruginosa P-5를 분리하였다. 이 균주는 nonanoic acid나 heptanoic acid와 같은 홀수개의 탄소수를 가지는 지방산을 단일 탄소원으로 공급해주었을 경우, 3-hydroxyvalerate (3HV)와 medium-chain-length (MCL) 3-hydroxyalkanoates 단위체로 이루어진 공중합체를 생산하였다. 공중합체 내 3HV의 함량은 valeric acid와 같은 보조기질을 공급함으로써 증가시킬 수 있었으며, 2 g/L nonanoic acid와 1 g/L valeric acid로 이루어진 혼합기질로부터 3HV의 함량이 26 mol%에 달하는 공중합체를 얻을 수 있었다. 이러한 공중합체는 결정성이 매우 낮아 점착성 고분자로서의 성질을 보였다. P. aeruginosa P-5 균주는 MCL-PHA synthase 유전자(phaC1, phaC2)를 가지고 있는 반면에 SCL-PHA synthase 유전자는 결여되어 있는 것으로 나타났다. 따라서 P. aeruginosa P-5 균주의 MCL-PHA synthase는 MCL(R)-3-hydroxyacyl-CoAs 뿐만 아니라 (R)-3-hydroxyvaleryl-CoA를 기질로 인지하는 특이한 기질특이성을 갖는 것으로 사료된다.

키워드

Pseudomonas aeruginosa;3-hydroxyvalerate;MCL-PHA synthase;polyhydroxyalkanoate

과제정보

연구 과제 주관 기관 : Chungnam National University

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피인용 문헌

  1. Substrate chain-length specificities of polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas aeruginosa P-5 vol.52, pp.4, 2016, https://doi.org/10.7845/kjm.2016.6055