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Biosynthesis of Copolyesters Consisting of 3-Hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates by the Pseudomonas aeruginosa P-5 Strain

Pseudomonas aeruginosa P-5 균주로부터 3-Hydroxyvalerate와 Medium-chain-length 3-hydroxyalkanoates로 구성된 공중합체의 생합성

  • Woo, Sang-Hee (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Kim, Jae-Hee (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Ni, Yu-Yang (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Rhee, Young-Ha (Department of Microbiology and Molecular Biology, Chungnam National University)
  • 우상희 (충남대학교 미생물.분자생명과학과) ;
  • 김재희 (충남대학교 미생물.분자생명과학과) ;
  • 예우양 (충남대학교 미생물.분자생명과학과) ;
  • 이영하 (충남대학교 미생물.분자생명과학과)
  • Received : 2012.07.31
  • Accepted : 2012.09.13
  • Published : 2012.09.30

Abstract

A bacterial strain capable of synthesizing polyhydroxyalkanoates (PHAs) with an unusual pattern of monomer units was isolated from activated sludge using the enrichment culture technique. The organism, identified as Pseudomonas aeruginosa P-5, produced polyesters consisting of 3-hydroxyvalerate and medium-chain-length (MCL) 3-hydroxyalkanoate monomer units when $C_{-odd}$ alkanoic acids such as nonanoic acid and heptanoic acid were fed as the sole carbon source. Solvent fractionation experiments using chloroform and hexane revealed that the 3-hydroxyalkanoate monomer units in these polyesters were copolymerized. The molar concentration of 3-hydroxyvalerate in the polyesters produced were significantly elevated up to 26 mol% by adding 1.0 g/L valeric acid as the cosubstrate. These copolyesters were sticky with low degrees of crystallinity. The PHA synthase genes were cloned, and the deduced amino acid sequences were determined. P. aeruginosa P-5 possessed genes encoding MCL-PHA synthases (PhaC1 and PhaC2) but lacked the short-chain-length PHA synthase gene, suggesting that the MCL-PHA synthases from P. aeruginosa P-5 are uniquely active for polymerizing (R)-3-hydroxyvaleryl-CoA as well as MCL (R)-3-hydroxyacyl-CoAs.

Keywords

Pseudomonas aeruginosa;3-hydroxyvalerate;MCL-PHA synthase;polyhydroxyalkanoate

Acknowledgement

Supported by : Chungnam National University

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