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Municipal Wastewater Treatment and Microbial Diversity Analysis of Microalgal Mini Raceway Open Pond

미세조류 옥외 배양시스템을 이용한 도시하수 정화 및 미생물 군집다양성 분석

  • Kang, Zion (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Byung-Hyuk (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Shin, Sang-Yoon (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Hee-Mock (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hee-Sik (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 강시온 (한국생명공학연구원 환경바이오연구센터) ;
  • 김병혁 (한국생명공학연구원 환경바이오연구센터) ;
  • 신상윤 (한국생명공학연구원 환경바이오연구센터) ;
  • 오희목 (한국생명공학연구원 환경바이오연구센터) ;
  • 김희식 (한국생명공학연구원 환경바이오연구센터)
  • Received : 2012.08.29
  • Accepted : 2012.09.20
  • Published : 2012.09.30

Abstract

Microalgal biotechnology has gained prominence because of the ability of microalgae to produce value-added products including biodiesel through photosynthesis. However, carbon and nutrient source is often a limiting factor for microalgal growth leading to higher input costs for sufficient biomass production. Use of municipal wastewater as a low cost alternative to grow microalgae as well as to treat the same has been demonstrated in this study using mini raceway open ponds. Municipal wastewater was collected after primary treatment and microalgae indigenous in the wastewater were encouraged to grow in open raceways under optimum conditions. The mean removal efficiencies of TN, TP, COD-$_{Mn}$, $NH_3$-N after 6 days of retention time was 80.18%, 63.56%, 76.34%, and 96.74% respectively. The 18S rRNA gene analysis of the community revealed the presence of Chlorella vulgaris and Scenedesmus obliquus as the dominant microalgae. In addition, 16S rRNA gene analysis demonstrated that Rhodobacter, Luteimonas, Porphyrobacter, Agrobacterium, and Thauera were present along with the microalgae. From these results, it is concluded that microalgae could be used to effectively treat municipal wastewater without aerobic treatment, which incurs additional energy costs. In addition, municipal wastewater shall also serve as an excellent carbon and nitrogen source for microalgal growth. Moreover, the microalgal biomass shall be utilized for commercial purposes.

Keywords

microalgae;microbial diversity;open culture system;wastewater treatment

Acknowledgement

Supported by : 교육과학기술부, 한국생명공학연구원

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