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Heavy Metal Detection and Removal in Artificial Wastewater Using Two-Component System Based Recombinant Bacteria

Two-component System 기반 재조합균을 이용한 인공폐수에서의 중금속 인지 및 제거

  • Ravikumar, Sambandam (School of Chemical Engineering and Bioengineering, University of Ulsan) ;
  • Hong, Soon-Ho (School of Chemical Engineering and Bioengineering, University of Ulsan) ;
  • Yoo, Ik-Keun (School of Chemical Engineering and Bioengineering, University of Ulsan)
  • ;
  • 홍순호 (울산대학교 공과대학 화학공학부) ;
  • 유익근 (울산대학교 공과대학 화학공학부)
  • Received : 2012.08.29
  • Accepted : 2012.09.18
  • Published : 2012.09.30

Abstract

Two-component system (TCS)-based bacterial zinc and copper biosensors, in which green fluorescent protein (GFP) is expressed under the control of zraP and cusC promoter in ZraS/R and CusS/R TCS, were evaluated in artificial wastewater. Bacterial biosensors developed in this study efficiently expressed GFP by the recognition of $Zn^{2+}$ and $Cu^{2+}$ in artificial wastewater. Secondly, TCS-based zinc and copper removing bacteria with the peptide displayed on cell surface were examined in artificial wastewater. Zinc and copper removing bacteria expressed the peptide as a fusion protein such as OmpC-ZBP (zinc binding peptide) and OmpC-CBP (copper binding peptide) on the cell surface when sensing exogenous $Zn^{2+}$ and $Cu^{2+}$ through ZraS/R and CusS/R TCS. The recombinant cell expressing metal-adsorbing peptide could efficiently remove copper and zinc (15 and 18 mg/g dry cell weight, respectively) in artificial wastewater. Therefore, it was demonstrated that the TCS-based recombinant cell for the recognition or removal of heavy metal functions well in artificial wastewater environment.

Keywords

Escherichia coli;artificial wastewater;biosensor;copper;zinc

Acknowledgement

Supported by : 한국연구재단

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