- Volume 13 Issue 6
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HER2/neu Expression in Head and Neck Squamous Cell Carcinoma Patients is not Significantly Elevated
- Sardari, Yasaman (Depatment of Oral and Maxillofacial Pathology, Dental School, Shiraz University of Medical Sciences) ;
- Pardis, Soheil (Depatment of Oral and Maxillofacial Pathology, Dental School, Shiraz University of Medical Sciences) ;
- Tadbir, Azadeh Andisheh (Depatment of Oral and Maxillofacial Pathology, Dental School, Shiraz University of Medical Sciences) ;
- Ashraf, Mohammad Javad (Department of Pathology, Medical School, Shiraz University of Medical Sciences) ;
- Fattahi, Mohammad Javad (Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences) ;
- Ebrahimi, Hooman (Department of Oral Medicine, Dental School, Shiraz University of Medical Sciences) ;
- Purshahidi, Sara (Department of Oral Medicine, Dental School, Shiraz University of Medical Sciences) ;
- Khademi, Bijan (Department of Otolaryngology, Khalili Hospital, Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences) ;
- Hamzavi, Marzieh (Depatment of Oral and Maxillofacial Pathology, Dental School, Shiraz University of Medical Sciences)
- Published : 2012.06.30
Background: HER2/neu, a member of EGFR family, is over expressed in some tumors. The purpose of this study was to determine the salivary level and tissue expression of HER2/neu in patients with head and neck squamous cell carcinoma (HNSCC) and any correlation with clinicopathologic parameters. Methods: An enzyme-linked immunosorbent assay (ELISA) was used to evaluate the salivary level and immunohistochemistry (IHC) to assess tissue expression of HER2/neu in 28 patients with HNSCC and 25 healthy controls. Results: The salivary levels of HER2/neu in HNSCC patients was not significantly higher than in the healthy controls (p>0.005). There was no apparent correlation in salivary HER2/neu level with clinicopathological features such as age, sex, grade, tumor size and nodal status. All HNSCC specimens were positive (membranous or/and cytoplasmic) for HER2/neu, except one sample. Only one HNSCC specimen was stained in cytoplasm purely. All control specimens were membranous and cytoplasmic positive for HER2/neu. There was a significant difference between cytoplasmic staining in case and control groups (p-value<0.05). Conclusion: In our cases, no overexpression of HER2/neu was observed. Thus, our findings suggested that the use of Her-2 as a salivary marker of HNSCC cannot be recommended.
HER2/neu;salivary level;tissue expression;head and neck squamous cell carcinoma
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