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Residue analysis of penicillines in livestock and marine products

국내 유통 축·수산물 중 페니실린계 동물용의약품에 대한 잔류실태조사

  • Song, Ji-Young (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration) ;
  • Hu, Soo-Jung (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration) ;
  • Joo, Hyun-Jin (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration) ;
  • Kim, Mi-Ok (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration) ;
  • Hwang, Joung-Boon (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration) ;
  • Han, Yoon-Jung (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration) ;
  • Kwon, Yu-Jihn (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration) ;
  • Kang, Shin-Jung (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration) ;
  • Cho, Dae-Hyun (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration)
  • 송지영 (경인지방식품의약품안전청 유해물질분석과) ;
  • 허수정 (경인지방식품의약품안전청 유해물질분석과) ;
  • 주현진 (경인지방식품의약품안전청 유해물질분석과) ;
  • 김미옥 (경인지방식품의약품안전청 유해물질분석과) ;
  • 황정분 (경인지방식품의약품안전청 유해물질분석과) ;
  • 한윤정 (경인지방식품의약품안전청 유해물질분석과) ;
  • 권유진 (경인지방식품의약품안전청 유해물질분석과) ;
  • 강신정 (경인지방식품의약품안전청 유해물질분석과) ;
  • 조대현 (경인지방식품의약품안전청 유해물질분석과)
  • Received : 2012.01.12
  • Accepted : 2012.08.09
  • Published : 2012.08.25

Abstract

Penicillins belong to the ${\beta}$-lactam class of antibiotics, and are frequently used in human and veterinary medicine. Despite the positive effects of these drugs, improper use of penicillins poses a potential health risk to consumers. This study has been undertaken to determinate multi-residues of penicillins, including amoxicillin, ampicillin, oxacillin, bezylpenicillin, cloxacillin, dicloxacillin, and nafcillin, using liquid chromatographic tandem mass spectrometer (LC-MS/MS). The developed method was validated for specificity, precision, recovery, and linearity in livestock and marine products. The analytes were extracted with 80% acetonitrile and clean-up by a single reversed-phase solid-phase extraction step. Six penicillins presented recoveries higher than 76% with the exception of Amoxicillin. Relative standard deviations (RSDs) were not more than 10%. The method was applied to 225 real samples. Benzylpenicillin was detected in 12 livestock products and 7 marine products. Amoxicillin, ampicillin, cloxacilllin, dicloxacillin, nafcillin and oxacillin were not detected. The detected levels were 0.001~0.009 mg/kg in livestock products excluding eggs and milk. In marine products, the detected levels were under 0.03 mg/kg. They were under the MRL levels. As monitoring results, it is identified to be safe but it is considered that safety management of antibiotics should continue by monitoring.

Keywords

livestock product;marine product;LC-MS/MS;monitoring;penicillins

Acknowledgement

Supported by : 식품의약품안전청

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