Degradation of Phytate Pentamagnesium Salt by Bacillus sp. T4 Phytase as a Potential Eco-friendly Feed Additive

  • Park, In-Kyung (Department of Animal Sciences and Environment, College of Animal Bioscience and Technology, Konkuk University) ;
  • Lee, Jae-Koo (Department of Animal Sciences and Environment, College of Animal Bioscience and Technology, Konkuk University) ;
  • Cho, Jaie-Soon (Department of Animal Sciences and Environment, College of Animal Bioscience and Technology, Konkuk University)
  • Received : 2012.05.18
  • Accepted : 2012.07.09
  • Published : 2012.10.01


A bacterial isolate derived from soil samples near a cattle farm was found to display extracellular phytase activity. Based on 16S rRNA sequence analysis, the strain was named Bacillus sp. T4. The optimum temperature for the phytase activity toward magnesium phytate (Mg-$InsP_6$) was $40^{\circ}C$ without 5 mM $Ca^{2+}$ and $50^{\circ}C$ with 5 mM $Ca^{2+}$. T4 phytase had a characteristic bi-hump two pH optima of 6.0 to 6.5 and 7.4 for Mg-$InsP_6$. The enzyme showed higher specificity for Mg-$InsP_6$ than sodium phytate (Na-$InsP_6$). Its activity was fairly inhibited by EDTA, $Cu^{2+}$, $Mn^{2+}$, $Co^{2+}$, $Ba^{2+}$ and $Zn^{2+}$. T4 phytase may have great potential for use as an eco-friendly feed additive to enhance the nutritive quality of phytate and reduce phosphorus pollution.


Supported by : Konkuk University


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