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Transfer of Orally Administered Terpenes in Goat Milk and Cheese

  • Poulopoulou, I. (Department of Nutritional Physiology and Feeding, Faculty of Animal Science and Aquaculture, Agricultural University of Athens) ;
  • Zoidis, E. (Department of Nutritional Physiology and Feeding, Faculty of Animal Science and Aquaculture, Agricultural University of Athens) ;
  • Massouras, T. (Department of Dairy Science, Faculty of Food Science and Technology, Agricultural University of Athens) ;
  • Hadjigeorgiou, Ioannis (Department of Nutritional Physiology and Feeding, Faculty of Animal Science and Aquaculture, Agricultural University of Athens)
  • Received : 2012.03.27
  • Accepted : 2012.05.18
  • Published : 2012.10.01

Abstract

The objective of the present study was to investigate the relationships between terpenes… intake and their presence in animal tissues (blood and milk) as well as in the final product (cheese). Eight dairy goats were divided in two balanced groups, representing control (C) and treatment (T) group. In T group oral administration of a mixture of terpenes (${\alpha}$-pinene, limonene and ${\beta}$-caryophyllene) was applied over a period of 18 d. Cheese was produced, from C and T groups separately, on three time points, twice during the period of terpenes… oral administration and once after the end of experiment. Terpenes were identified in blood by extraction using petroleum ether and in milk and cheese by the use of solid phase micro-extraction (SPME) method, followed by GC-MS analysis. Chemical properties of the milk and the produced cheeses were analyzed and found not differing between the two groups. Limonene and ${\alpha}$-pinene were found in all blood and milk samples of the T group after a lag-phase of 3 d, while ${\beta}$-caryophyllene was determined only in few milk samples. Moreover, none of the terpenes were traced in blood and milk of C animals. In cheese, terpenes' concentrations presented a more complicated pattern implying that terpenes may not be reliable feed tracers. We concluded that monoterpenes can be regarded as potential feed tracers for authentification of goat milk, but further research is required on factors affecting their transfer.

Keywords

Feed Tracers;Terpenes;Goat;Blood;Milk;Cheese

References

  1. Abilleira, E., M. de Renobales, A. I. Najera, M. Virto, J. C. R. D. Gordoa, F. J. Perez-Elortondo, M. Albisu and L. J. R. Barron. 2010. An accurate quantitative method for the analysis of terpenes in milk fat by headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry. Food Chem. 120:1162-1169. https://doi.org/10.1016/j.foodchem.2009.11.050
  2. Ait Said, S., C. Fernandez, S. P. Greff, A. Derridj, T. Gauquelin and J. P. Mevy. 2011. Inter-population variability of leaf morpho-anatomical and terpenoid patterns of Pistacia atlantica Desf. ssp. atlantica growing along an aridity gradient in Algeria. Flora 206:397-405. https://doi.org/10.1016/j.flora.2010.08.002
  3. Boyle, R. R., S. McLean, S. Brandon, G. J. Pass and N. W. Davies. 2002. Application of solid-phase microextraction to the quantitative analysis of 1,8-cineole in blood and expired air in a Eucalyptus herbivore, the brushtail possum (Trichosurus vulpecula). J. Chromatogr. B. 780:397-406. https://doi.org/10.1016/S1570-0232(02)00625-6
  4. Boyle, R. R., S. McLean, S. Brandon and N. Wiggins. 2005. Rapid absorption of dietary 1, 8-cineole results in critical blood concentration of cineole and immediate cessation of eating in the common brushtail possum (Trichosurus vulpecula). J. Chem. Ecol. 31:2775-2790. https://doi.org/10.1007/s10886-005-8393-0
  5. Clericuzio, M., G. Alagona, C. Ghio and L. Toma. 2000. Ab initio and density functional evaluations of the molecular conformations of $\beta$-caryophyllene and 6-hydroxycaryophyllene. J. Org. Chem. 65:6910-6916. https://doi.org/10.1021/jo000404+
  6. Cornu, A., N. Kondjoyan, B. Martin, I. Verdier-Metz, P. Pradel, J. L. Berdague and J. B. Coulon. 2005. Terpene profiles in Cantal and Saint-Nectaire-type cheese made from raw or pasteurised milk. J. Sci. Food Agric. 85:2040-2046. https://doi.org/10.1002/jsfa.2214
  7. Coulon, J. B., A. Delacroix-Buchet, B. Martin and A. Pirisi. 2004. Relationships between ruminant management and sensory characteristics of cheeses: A review. Lait 84:221-241. https://doi.org/10.1051/lait:2004008
  8. Dalvit, C., M. De Marchi and M. Cassandro. 2007. Genetic traceability of livestock products: A review. Meat Sci. 77:437-449. https://doi.org/10.1016/j.meatsci.2007.05.027
  9. De Carvalho, C. C. C. R. and M. M. R. Da Fonseca. 2006. Biotransformation of terpenes. Biotechnol. Adv. 24:134-142. https://doi.org/10.1016/j.biotechadv.2005.08.004
  10. De Noni, I. and G. Battelli. 2008. Terpenes and fatty acid profiles of milk fat and "Bitto" cheese as affected by transhumance of cows on different mountain pastures. Food Chem. 109:299-309. https://doi.org/10.1016/j.foodchem.2007.12.033
  11. Duncan, A. J., S. E. Hartley and G. R. Iason. 1994. The effect of monoterpene concentrations in Sitka spruce (Picea sitchensis) on the browsing behaviour of red deer (Cervus elaphus). Can. J. Zool. 72:1715-1720. https://doi.org/10.1139/z94-231
  12. Duncan, A. J. and D. P. Poppi. 2008. Nutritional ecology of grazing and browsing ruminants. In: The Ecology of Browsing and Grazing (Ed. I. J. Gordon and H. H. T. Prins). Ecological Studies 195:89-116. https://doi.org/10.1007/978-3-540-72422-3_4
  13. Dziba, L. E. and F. D. Provenza. 2008. Dietary monoterpene concentrations influence feeding patterns of lambs. Appl. Anim. Behav. Sci. 109:49-57. https://doi.org/10.1016/j.applanim.2007.02.003
  14. Estell, R. E. 2010. Coping with shrub secondary metabolites by ruminants. Small Rumin. Res. 94:1-9. https://doi.org/10.1016/j.smallrumres.2010.09.012
  15. Fedele, V., S. Claps, R. Rubino, L. Sepe and G. F. Cifuni. 2004. Variation in terpene content and profile in milk in relation to the dominant plants in the diet of grazing goats. S. Afr. J. Anim. Sci. 34:145-147.
  16. Hadjigeorgiou, I. E., I. J. Gordon and J. A. Milne. 2003. Comparative preference by sheep and goats for Graminaeae forages varying in chemical composition. Small Rumin. Res. 49:147-156. https://doi.org/10.1016/S0921-4488(03)00094-4
  17. Hofmann, R. R. 1989. Evolutionary steps of ecophysiological adaptation and diversification of ruminants: a comparative view of their digestive system. Oecologia 78:443-457. https://doi.org/10.1007/BF00378733
  18. IDF (International Dairy Federation). 1982. Cheese and processed cheese-total solids content (Reference Method). Standard FIL-IDF 4A. Brussels, Belgium.
  19. IDF (International Dairy Federation). 1991. Milk and milk products. Fat Content. General Guidance on the use of Butyrometric Methods. Standard FIL-IDF 52. Brussels, Belgium.
  20. IDF (International Dairy Federation). 1993. Milk Nitrogen Content. Standard FIL-IDF 220B. Brussels, Belgium.
  21. Llusia, J., G. Penuelas, A. Alessio and M. Estiarte. 2006. Seasonal contrasting changes of foliar concentrations of terpenes and other volatile organic compound in four dominant species of a Mediterranean shrubland submitted to a field experimental drought and warming. Physiol. Plant 127:632-649. https://doi.org/10.1111/j.1399-3054.2006.00693.x
  22. Malecky, M., L. P. Broudiscou and P. Schmidely. 2009. Effects of two levels of monoterpene blend on rumen fermentation, terpene and nutrient flows in the duodenum and milk production in dairy goats. Anim. Feed Sci. Technol. 154:24-35. https://doi.org/10.1016/j.anifeedsci.2009.07.004
  23. Mariaca, R. G., T. F. H. Berger, R. Gauch, M. I. Imhof, B. Jeangros and J. O. Bosset. 1997. Occurrence of volatile mono- and sesquiterpenoids in highland and lowland plant species as possible precursors for flavor compounds in milk and dairy products. J. Agric. Food Chem. 45:4423-4434. https://doi.org/10.1021/jf970216t
  24. Martin, B., I. Verdier-Metz, S. Buchin, C. Hurtaud and J. B. Coulon. 2005. How do the nature of forages and pasture diversity influence the sensory quality of dairy livestock products? Anim. Sci. 81:205-212.
  25. McLean, S., S. Brandon, R. R. Boyle and N. Wiggins. 2008. Development of tolerance to the dietary plant secondary metabolite 1,8-cineole by brushtail possum (Trichosurus vulpecula). J. Chem. Ecol. 34:672-680. https://doi.org/10.1007/s10886-008-9463-x
  26. McLean, S. and A. J. Duncan. 2006. Pharmacological perspectives on the detoxification of plant secondary metabolites: Implications for ingestive behavior of herbivores. J. Chem. Ecol. 32:1213-1228. https://doi.org/10.1007/s10886-006-9081-4
  27. McLean, S., J. W. Foley, W. N. Davies, S. Brandon, L. Duo and J. A. Blackman. 1993. Metabolic fate of dietary terpenes from Eucalyptus radiata in common ringtail possum (Pseudocheirus peregrinus). J. Chem. Ecol. 19:1625-1643. https://doi.org/10.1007/BF00982297
  28. Morand-Fehr, P., V. Fedele, M. Decandia and Y. Le Frileux. 2007. Influence of farming and feeding systems on composition and quality of goat and sheep milk. Small Rumin. Res. 68:20-34. https://doi.org/10.1016/j.smallrumres.2006.09.019
  29. Poulopoulou, I., E. Zoidis, T. Massouras and I. Hadjigeorgiou. 2011. Terpenes transfer to milk and cheese after oral administration to sheep fed indoors. J. Anim. Physiol. Anim. Nutr. ( In press DOI: 10.1111/j.1439-0396.2011.01128.x).
  30. Prache, S., A. Cornu, J. L. Berdague and A. Priolo. 2005. Traceability of animal feeding diet in the meat and milk of small ruminants. Small Rumin. Res. 59:157-168. https://doi.org/10.1016/j.smallrumres.2005.05.004
  31. Priolo, A., A. Cornu, S. Prache, M. Krogmann, N. Kondjoyan, D. Micol and J. L. Berdague. 2004. Fat volatiles tracers of grass feeding in sheep. Meat Sci. 66:475-481. https://doi.org/10.1016/S0309-1740(03)00136-0
  32. Revello-Chion, A., E. Tabacco, D. Giaccone, P. G. Peiretti, G. Battelli and G. Borreani. 2010. Variation of fatty acid and terpene profiles in mountain milk and "Toma piemontese" cheese as affected by diet composition in different seasons. Food Chem. 121:393-399. https://doi.org/10.1016/j.foodchem.2009.12.048
  33. Spencer, J. P. E., M. M. Abd El Mohsen, A. M. Minihane and J. C. Mathers. 2008 Biomarkers of the intake of dietary polyphenols: Strengths, limitations and application in nutrition research. Br. J. Nutr. 99:12-22.
  34. Statgraphics Plus, 2000. User's guide: Version. 2.7. Manugistics. Rockville, MA, USA.
  35. Tornambe, G., A. Cornu, P. Pradel, N. Kondjoyan, A. P. Carnat, M. Petit and B. Martin. 2006. Changes in terpene content in milk from pasture-fed cows. J. Dairy Sci. 89:2309-2319. https://doi.org/10.3168/jds.S0022-0302(06)72302-5
  36. Tsiplakou, E., I. Hadjigeorgiou, K. Sotirakoglou and G. Zervas. 2011. Differences in mean retention time of sheep and goats under controlled feeding practices. Small Rumin. Res. 95:48-53. https://doi.org/10.1016/j.smallrumres.2010.09.002
  37. Utsumi, S. A., A. F. Cibils, R. E. Estell, S. A. Soto-Navarro and D. Van Leeuwen. 2009. Seasonal changes in one seed juniper intake by sheep and goats in relation to dietary protein and plant secondary metabolites. Small Rumin. Res. 81:152-162. https://doi.org/10.1016/j.smallrumres.2008.12.011
  38. Vasta, V., A. Nudda, A. Cannas, M. Lanza and A. Priolo. 2008. Alternative feed resources and their effects on the quality of meat and milk from small ruminants. Anim. Feed Sci. Technol. 147:223-246. https://doi.org/10.1016/j.anifeedsci.2007.09.020
  39. Viallon, C., B. Martin, I. Verdier-Metz, P. Pradel, J. P. Garel, J. B. Coulon and J. L. Berdague. 2000. Transfer of monoterpenes and sesquiterpenes from forages into milk fat. Lait 80:635-641. https://doi.org/10.1051/lait:2000150
  40. Viallon, C., I. Verdier-Metz, C. Denoyer, P. Pradel, J. B. Coulon and J. L. Berdague. 1999. Desorbed terpenes and sesquiterpenes from forages and cheeses. J. Dairy Res. 66:319-326. https://doi.org/10.1017/S0022029999003520
  41. Villalba, J. J., F. D. Provenza and K. C. Olson. 2006. Terpenes and carbohydrate source influence rumen fermentation, digestibility, intake, and preference in sheep. J. Anim. Sci. 84:2463-2473. https://doi.org/10.2527/jas.2005-716

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