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Anti-wrinkle Compounds Isolated from the Seeds of Arctium lappa L.

우방자에서 분리한 주름개선 화합물

  • Hwang, Ju-Young (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Park, Tae-Soon (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Kim, Dong-Hee (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Hwang, Eun-Young (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Lee, Jung-Noh (Coreana Cosmetics Co. Ltd. Songpa R&D Center) ;
  • Lee, Ji-Young (Coreana Cosmetics Co. Ltd. Songpa R&D Center) ;
  • Lee, Ghang-Tai (Coreana Cosmetics Co. Ltd. Songpa R&D Center) ;
  • Lee, Kun-Kook (Coreana Cosmetics Co. Ltd. Songpa R&D Center) ;
  • Son, Jun-Ho (Korea Promotion Institute for Traditional Medicine Industry)
  • Received : 2012.06.27
  • Accepted : 2012.08.22
  • Published : 2012.08.30

Abstract

This study was carried out to discover the skin wrinkle reducing components in the seeds of Arctium lappa. The isolation of a methylene chloride-soluble fraction of 70% ethanol extract from the seeds of Arctium lappa using a procollagen type-1 synthesis and MMP-1 activity resulted in the isolation and identification of four lignin compounds: arctiin, arctigenin, matairesinol, and diarctigenin. All structures were confirmed via NMR and MS spectroscopic data. To determine cell viability and procollagen type-1 synthesis, human dermal fibroblasts were treated with 10-100 ${\mu}M$. As a result, none of the four compounds showed cytotoxicity up to 50 ${\mu}M$. We also investigated their procollagen type-1 synthesis and MMP-1 inhibition activity and found that arctiin had the highest activity in terms of both procollagen synthesis and MMP-1 inhibition among all four compounds. Putting all the data together, we suggest that arctiin be used in cosmetics as an anti-wrinkle material.

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