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치아우식증 및 치주질환 원인균에 대한 Carvacrol의 항균효과

Antimicrobial Effect of Carvacrol against Cariogenic and Periodontopathic Bacteria

  • 박순낭 (조선대학교 치과대학 구강생화학교실) ;
  • 이동균 (조선대학교 치과보존학교실) ;
  • 임윤경 (조선대학교 치과대학 구강생화학교실) ;
  • 김화숙 (전남과학대학 치위생과) ;
  • 조유진 (조선대학교 치과대학 구강생화학교실) ;
  • 김동춘 (중국 연변대학교 농학원 동물의학교실) ;
  • 김생곤 (조선대학교 치과대학 인체생물학교실) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실)
  • Park, Soon-Nang (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Lee, Dong-Kyun (Department of Conservative Dentistry, School of Dentistry, Chosun University) ;
  • Lim, Yun-Kyong (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Kim, Hwa-Sook (Department of Dental Hygiene, Chunnam Techno College) ;
  • Cho, Eu-Gene (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Jin, Dongchun (Department of Veterinary Medicine, College of Agriculture, Yanbian University) ;
  • Kim, Saeng-Gon (Department of Human Biology, School of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, School of Dentistry, Chosun University)
  • 투고 : 2012.02.13
  • 심사 : 2012.03.21
  • 발행 : 2012.03.31

초록

본 연구는 치아우식증 및 치주질환 원인균들에 대한 carvacrol의 항균능을 알아보고, 사람의 구강조직 세포에서 세포의 생존율을 분석하여 세포독성 정도를 알아보기 위해 실시하였다. Carvacrol의 항균능은 뮤탄스 연쇄상구균과 5종류의 치주질환 원인균을 이용하여 MIC 및 MBC 값을 측정하여 분석하였고, 세포생존율은 정상 사람 치은섬유모세포를 이용하여 MTT 분석법으로 평가하였다. 구강질환 원인균에 대한 carvacrol의 MIC 및 MBC 값은 각각 $16-128{\mu}g/ml$$32-128{\mu}g/ml$이었다. 세포독성 실험 결과 carvacrol은 $128{\mu}g/ml$ 농도에서 세포생존율이 현저히 감소하였다. 따라서 carvacrol은 치아우식증과 치주질환 원인균에 대한 항균작용이 뛰어나지만, 구강조직 세포에 대해 세포독성을 가지고 있어 가글린제 및 치약 등의 구강위생용품에 활용하기 위해서는 $64{\mu}g/ml$ 이하의 농도로 사용하고 정상 사람 치은섬유모세포에 대한 독성이 없는 다른 항균물질과 혼합하여 사용하는 것이 효과적일 것으로 생각된다.

키워드

antimicrobial effect;carvacrol;mutans streptococci;periodontopathogens

과제정보

연구 과제 주관 기관 : 보건복지가족부

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피인용 문헌

  1. Antimicrobial Effects of Ethanol Extract of Yongdamgosam-hwan against Streptococcus mutans vol.30, pp.6, 2015, https://doi.org/10.6116/kjh.2015.30.6.55.