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Effect of Mature Miscanthus sacchariflorus var. No. 1 on In Vitro Rumen Fermentation Characteristics and Its Dry Matter Digestibility

생육 후기 거대억새의 In vitro 반추위 발효특성 및 건물 소화율

  • Cho, Sang-Buem (Department of Animal Science, Chonbuk National University) ;
  • Mbiriri, David Tinotenda (Department of Animal Science, Chonbuk National University) ;
  • Oh, Sung-Jin (Department of Animal Science, Chonbuk National University) ;
  • Lee, A-Reum (Department of Animal Science, Chonbuk National University) ;
  • Yang, Jin-Ho (Department of Animal Science, Chonbuk National University) ;
  • Ryu, Chae-Hwa (Department of Animal Science, Chonbuk National University) ;
  • Park, Chang-Min (Department of Animal Science, Chonbuk National University) ;
  • Moon, Yun-Ho (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Chae, Jung-Il (Department of Oral Pharmacology, School of Dentistry and Institute of Dental Bioscience, Chonbuk National University) ;
  • Choi, Nag-Jin (Department of Animal Science, Chonbuk National University)
  • Received : 2012.05.31
  • Accepted : 2012.06.18
  • Published : 2012.06.30

Abstract

This study was conducted to develop Miscanthus as a new roughage resource for ruminant animals. Miscanthus sacchariflorus var. No 1, a newly developed germtype in Korea, was harvested at late maturity stage and its effect on rumen pH, ammonia nitrogen, gas production, volatile fatty acid (VFA) production and digestibility were evaluated using in vitro rumen fermentation. The effects of Miscanthus were compared with rice straw. Miscanthus showed significantly higher pH compared to rice straw (p<0.01). As for ammonia nitrogen, there was no significant difference after 12 h of incubation (p>0.05). Gas production in Miscanthus was significantly lower than that of rice straw in overall incubation time (p<0.05) after 6 h of incubation. In VFA production, acetate, propionate, butyrate, valerate and total VFA production in Miscanthus were lower than those in rice straw. However, production of iso-butyrate and iso-valerate were not different in between two forage materials. Dry matter digestibility of Miscanthus was significantly lower than rice straw (p<0.05) during 12~24 h of incubation. As a result, the availability of Miscanthus as roughage source showed approximately 80% that of rice straw.

Acknowledgement

Grant : Cooperative Research Program for Agriculture Science & Technology Development

Supported by : Rural Development Administration

References

  1. A.O.A.C. 1995. Official methods of analysis 16th edition. Association of official analytical chemist (Washington, D.C).
  2. Bae, D.H., B.E. Gilman, J.G. Welch and R.H. Palmer. 1983. Quality of forage from Miscanthus sinensis. J. Dairy Sci. 66:630-633. https://doi.org/10.3168/jds.S0022-0302(83)81835-9
  3. Chaney, A.L. and E.P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8:130-132.
  4. Erwin, E.S., G.J. Marco and E.M. Emery. 1961. Volatile fatty acid analyses of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44: 1768-1771. https://doi.org/10.3168/jds.S0022-0302(61)89956-6
  5. Heather, Y. and C. Somerville. 2012. Development of feedstocks for cellulosic biofuels. F1000 Reports Biology 02: doi:10.3410/B4-10.
  6. Hiltner, P. and B.A. Dehority. 1983. Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria. Appl. Environ. Microbiol. 46:642-648.
  7. Kook, K., B.C. Lee, W.H. Kim, K.Y. Jang, K.S. Back, S.J. Moon and G. H. Kim. 2011. Effects of whole crop barley silage (WBS) supplementation on growth performance and meat quality of Hanwoo steers. Korean J. Food Sci. Anim. Resour. 31:107-114. https://doi.org/10.5851/kosfa.2011.31.1.107
  8. Lee, I.D. and H.S. Lee. 2008. Study on the food habits of Korean native goats (Capra hircus) fed with various roughage sources. J. Kor. Grassl. Forage Sci. 28:119-128. https://doi.org/10.5333/KGFS.2008.28.2.119
  9. Lewandowski, I., J.C. Clifton-Brown, J. M. O. Scurlock and W. Huisman. 2000. Miscanthus: European experience with a novel energy crop. Biomass Bioenergy 19: 209-227. https://doi.org/10.1016/S0961-9534(00)00032-5
  10. Moon, Y.H., B.C. Koo, Y.H. Choi, S.H. Ahn, S. T. Bark, Y.L. Cha, G.H. An, J.K. Kim and S.J. Suh. 2010. Development of "Miscanthus" the promising bioenergy crop. Kor. J. Weed Sci. 30: 330-339. https://doi.org/10.5660/KJWS.2010.30.4.330
  11. Moore, J.E. 1970. Procedures for the two-stage in vitro digestion of forages. 3. In L.E. Harris (ed) Vol. 1. Nutrition research techniques for domestic and wild animlas (Utah State Univ., Logan, UT).
  12. Park, J.K., D.H. Lim, S.B. Kim, K.S. Ki, H.J. Lee, E.G. Kwon, W.M. Cho and C.H. Kim. 2011. Effects of partial replacement of corn grain and soybean meal with agricultural by-product feed on in vitro rumen fermentation chracteristics and optimum levels of mixing ratio. J. Anim. Sci. & Technol. (Kor.) 53:441-450. https://doi.org/10.5187/JAST.2011.53.5.441
  13. Perdok, H. and R.A. Leng. 1989. Rumen ammonia requirements for efficient digestion and intake of straw by cattle. In: The Role of Protozoa and Fungi in Ruminant Digestion (Ed. J.V. Nolan and R.A. Leng) (Penambul Books, Armidale, Australia).
  14. Ramirez, C.E., H. Kumagai, E. Hosoi, F. Yano, H. Yano, K.K. Jung and S.W. Kim. 1994. Mineral concentration in rice straw and soil in Kyongbuk Province, Korea. Asian-Aust. J. Anim. Sci. 7:125-129. https://doi.org/10.5713/ajas.1994.125
  15. Stewart, C.S. 1977. Factors affecting the cellulolytic activity of rumen contents. Appl. Environ. Microbiol. 33: 497-502.
  16. Van Soest, P.J. 2006. Rice straw, the role of silica and treatments to improve quality. Anim. Feed Sci. Technol. 130:137-171. https://doi.org/10.1016/j.anifeedsci.2006.01.023