Effects on Biomarkers and Endocrine in Muddy Loach (Misgurnus anguillicaudatus) under 21 day Exposure to Methomyl

21일간 methomyl에 노출한 미꾸리의 생물지표 및 내분비계 영향

  • Han, Sun-Young (Environmental Toxicology Research Center, Korea Institute of Toxicology) ;
  • Kim, Ja-Hyun (Environmental Toxicology Research Center, Korea Institute of Toxicology) ;
  • Gwon, Ga-Young (Environmental Toxicology Research Center, Korea Institute of Toxicology) ;
  • Yeom, Dong-Hyuk (Environmental Toxicology Research Center, Korea Institute of Toxicology)
  • 한선영 (안전성평가연구소 환경독성연구센터) ;
  • 김자현 (안전성평가연구소 환경독성연구센터) ;
  • 권가영 (안전성평가연구소 환경독성연구센터) ;
  • 염동혁 (안전성평가연구소 환경독성연구센터)
  • Received : 2011.12.28
  • Accepted : 2012.02.20
  • Published : 2012.03.31


To evaluate the effect of endocrine disruption chemicals (EDCs) to aquatic organisms, muddy loach (Misgurnus anguillicaudatus) was exposed to low concentration methomyl for 21 days in order to identify the effect of biomarkers and endocrine. Vitellogenin (VTG) in blood plasma, which used widely as validated biomarker for endocrine disruption, was significantly greater in male fish exposed to 0.4 mg/L and 2 mg/L methomyl, and in female fish exposed to 0.08 mg/L, 0.4 mg/L, and 2 mg/L methomyl for 21 days (p<0.05). This results suggest that methomyl have probability of endocrine disruption to organism on aquatic system. While inhibition of Acetylcholinesterase (AChE) activity and increase of DNA damage in comet assay were verified by fish exposed to methomyl, change of ethoxyresorufin-O-deethylase (EROD) activity was not occurred, comparing the control group (p<0.05). Indicators at the level of organism such as condition factor (CF), hepato-somatic index (HSI), and gonado-somatic index (GSI) were not influenced by exposure of methomyl. In conclusion, these results showed the possibility of methomyl in regard to not only endocrine disruption but also impacts on biochemical biomarkers to aquatic organisms.


Methomyl;Misgurnus anguillicaudatus;Vitellogenin;Biochemical biomarkers;Endocrine disruption


Supported by : 농촌진흥청


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