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Analysis of Mitochondrial Gene Sequence in Etoxazole Resistant Two-Spotted Spider Mite, Tetranychus urticae

Etoxazole 저항성 점박이응애의 미토콘드리아 유전자 서열 분석

  • Park, Sang-Eun (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Koo, Hyun-Na (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Yoon, Chang-Mann (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Choi, Jang-Jeon (Pear Research Station, NIHHS, RDA) ;
  • Kim, Gil-Hah (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 박상은 (충북대학교 농업생명환경대학 식물의학과) ;
  • 구현나 (충북대학교 농업생명환경대학 식물의학과) ;
  • 윤창만 (충북대학교 농업생명환경대학 식물의학과) ;
  • 최장전 (농촌진흥청 국립원예특작과학원 배시험장) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2011.11.07
  • Accepted : 2012.01.30
  • Published : 2012.03.31

Abstract

The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is one of the most important pest species devastating many horticultural and ornamental crops and fruit trees. Difficulty in managing this mite is largely attributed to its ability to develop resistance to many important acaricides. Development of 3,700-folds resistance to etoxazole was found in the population of T. urticae collected from rose greenhouses in Buyeo, Chungnam Province in August 2000. This population has been selected for eleven years with etoxazole (over 500 times), and increased over 5,000,000-folds in resistance as compared with susceptible strain. Also, etoxazole-resistant strain was shown to be maternally inherited. The objective of this study was to determine whether resistance of T. urticae to etoxazole was linked with point mutations in the mitochondrial gene. DNA sequencing of cytochrome c oxidase subunit I (COX1), COX2, COX3, cytochrome b (CYTB), NADH dehydrogenase subunit 1 (ND1), ND2, ND3, ND4, ND5, and ND6 were analyzed by comparing two etoxazole-susceptible and etoxazole-resistant strains. As a result, differences were not detected between the nucleotide sequences of two strains within a mitochondrial gene.

Keywords

Tetranychus urticae;Etoxazole;Mitochondrial gene;Point mutation

Acknowledgement

Grant : 원예작물 경쟁력 제고 기술 - 배 수출증대를 위한 규격과 생산 및 주요 병해충 방제기술 개발 연구

Supported by : 농촌진흥청

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