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Cytokines Expression and Nitric Oxide Production under Induced Infection to Salmonella Typhimurium in Chicken Lines Divergently Selected for Cutaneous Hypersensitivity

  • Singh, Rani (Disease Genetics and Biotechnology Laboratory, Central Avian Research Institute) ;
  • Jain, Preeti (Disease Genetics and Biotechnology Laboratory, Central Avian Research Institute) ;
  • Pandey, N.K. (Disease Genetics and Biotechnology Laboratory, Central Avian Research Institute) ;
  • Saxena, V.K. (Disease Genetics and Biotechnology Laboratory, Central Avian Research Institute) ;
  • Saxena, M. (Disease Genetics and Biotechnology Laboratory, Central Avian Research Institute) ;
  • Singh, K.B. (Department of Animal Science, M. J. P. Rohilkhand University) ;
  • Ahmed, K.A. (Disease Genetics and Biotechnology Laboratory, Central Avian Research Institute) ;
  • Singh, R.P. (Disease Genetics and Biotechnology Laboratory, Central Avian Research Institute)
  • Received : 2011.09.09
  • Accepted : 2011.12.28
  • Published : 2012.07.01

Abstract

In the present study, the impact of Salmonella Typhimurium on cell-mediated immunity (CMI) was investigated in 5 week-old immuno divergent broiler lines selected for the high and low response to phytohemagglutinin-P. The immune response was assessed in peripheral-blood mononuclear cells (PBMCs) induced with Salmonella Typhimurium at different time intervals (0 h, 0.5 h, 2 h, 4 h, 6 h, 12 h and 24 h). The differential mRNA expression patterns of IFN-${\gamma}$, IL-2 and iNOS were evaluated by quantitative real time PCR. In-vitro production of nitric oxide (NO) was also estimated in the culture supernatant and correlated with iNOS mRNA expression. Present study showed higher production of NO in the high cell-mediated line (HCMI) as compared to the low cell-mediated line (LCMI) upon stimulation with Salmonella Typhimurium. Correspondingly, higher mRNA expression of iNOS and IFN-${\gamma}$ were observed in high response birds (HCMI); but IL-2 was down regulated in this line compared to the low response birds (LCMI). Significantly (p<0.05) higher expression of iNOS, IFN-${\gamma}$ and higher production of NO in high line indicated that the selection for PHA-P response might be employed for increasing the immune competence against Salmonella Typhimurium in chicken flocks.

Keywords

Phytohaemagglutinin-P (PHA-P);Nitric Oxide (NO);Peripheral Blood Mononuclear Cells (PBMCs)

Acknowledgement

Supported by : Ministry of Science and Technology of the Government of India

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