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Substantial Effect of Melanin Influencing Factors on In vitro Melanogenesis in Muzzle Melanocytes of Differently Colored Hanwoo

  • Amna, Touseef (Department of Animal Science and Institute of Rare Earth for Biological Application, Chonbuk National University) ;
  • Park, Kyoung-Mi (Department of Animal Science and Institute of Rare Earth for Biological Application, Chonbuk National University) ;
  • Cho, In-Kyung (Department of Food and Nutrition, Nambu University) ;
  • Choi, Tae-Jeong (National Institute of Animal Science, RDA) ;
  • Lee, Seung-Soo (National Institute of Animal Science, RDA) ;
  • Seo, Kang-Seok (Department of Animal Science and Technology, Suncheon National University) ;
  • Hwang, In-Ho (Department of Animal Science and Institute of Rare Earth for Biological Application, Chonbuk National University)
  • Received : 2011.08.19
  • Accepted : 2012.01.30
  • Published : 2012.07.01

Abstract

The present study was designed to investigate the effect of ${\alpha}$-melanocyte-stimulating hormone (${\alpha}$-MSH), nitric oxide (NO) and L-cysteine on melanin production and expression of related genes MC1R, Tyr, Tyrp-1 and Tyrp-2 in muzzle melanocytes of differently colored three native Hanwoo cattle. Muzzle samples were taken from black, brindle and brown Hanwoo and purified melanocytes were cultured with ${\alpha}$-MSH, nitric oxide and L-cysteine at 100 nM, $50{\mu}M$ and 0.07 mg/ml of media respectively. The amounts of total melanin, eumelanin and mRNA expression at Tyr, Tyrp-1, Tyrp-2 and MC1R levels were quantified. ${\alpha}$-MSH and nitric oxide significantly increased (p<0.05) the amount of total melanin in black and brindle whereas eumelanin production in brown Hanwoo muzzle melanocytes. On the contrary, L-cysteine greatly (p<0.05) depressed the eumelanin production in black color but increased in brown. Simultaneously, up regulation of Tyr by nitric oxide and ${\alpha}$-MSH and down regulation of Tyr, Tyrp-2 and MC1R genes by L-cysteine were observed in muzzle melanocytes of all three phenotypes. The results of this study revealed nitric oxide and ${\alpha}$-MSH contribute hyper-pigmentation by enhancing eumelanogenesis whereas L-cysteine contributes to pheomelanin production in different colored Hanwoo muzzle melanocytes.

Keywords

Melanogenesis;Nitric Oxide;Hanwoo Muzzle;Melanocytes;Cysteine

Acknowledgement

Supported by : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries, Rural Development Administration

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