- Volume 25 Issue 7
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Differential Expression of Th1- and Th2- Type Cytokines in Peripheral Blood Mononuclear Cells of Murrah Buffalo (Bubalus Bubalis) on TLR2 Induction by B. Subtilis Peptidoglycan
- Shah, Syed M. (Post-Graduate Institute of Veterinary Education and Research, Guru Angad Dev Veterinary and Animal Sciences University) ;
- Ravi Kumar, G.V.P.P.S. (Post-Graduate Institute of Veterinary Education and Research, Guru Angad Dev Veterinary and Animal Sciences University) ;
- Brah, G.S. (Post-Graduate Institute of Veterinary Education and Research, Guru Angad Dev Veterinary and Animal Sciences University) ;
- Santra, Lakshman (Post-Graduate Institute of Veterinary Education and Research, Guru Angad Dev Veterinary and Animal Sciences University) ;
- Pawar, Hitesh (Post-Graduate Institute of Veterinary Education and Research, Guru Angad Dev Veterinary and Animal Sciences University)
- Received : 2012.01.13
- Accepted : 2012.03.29
- Published : 2012.07.01
Peripheral blood mononuclear cells (PBMCs) discriminate microbial pathogens and induce T-cell responses of appropriate effector phenotype accordingly. Toll-like receptors (TLRs), in part, mediate this microbial recognition and differentiation while the development of T-cell effector functions critically depends on the release of Th1- or Th2- type cytokines. In the present study, buffalo PBMCs were stimulated under in vitro culture conditions by Bacillus subtilis cell wall petidoglycan, a TLR2 ligand, in a dose- and time- dependent manner. The expression of TLR2 as well as the subsequent differential induction of the Th1 and Th2 type cytokines was measured. Stimulation was analyzed across five doses of peptidoglycan (
Differential Cytokine Induction;Buffalo PBMCs;Th1 and Th2 Type Cytokines;Bacillus subtilis Peptidoglycan
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